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PCR-RFLP analysis of the whole chloroplast DNA from three cultivated species of cotton (Gossypium L.)

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Abstract

Variations of the chloroplast DNA (cpDNA) from three of the four cultivated species of cotton (Malvaceae); Gossypium barbadense L., Gossypium hirsutum L., Gossypium arboreum L. and its synonym Gossypium nanking Meyen., were analyzed. Using specific set of primers, the whole circular cpDNAs from the four test species were amplified. These were subsequently digested with the use of seven restriction enzymes. The amplified fragments of the whole cpDNAs of the diploid cultivated cotton G. arboreum and its synonym G. nanking did not show any differences. However, the allotetraploid cultivated cottons G. barbadense and G. hirsutum, showed some fragment length differences directly visible after amplification and two types of restriction fragment length polymorphism (RFLP), the first appeared as slightly lengthened bands and the other as gain or loss of a restriction site. The results also showed that the chloroplast genomes of the allotetraploid cultivated cottons are highly similar to the diploid cultivated cottons tested in terms of length and digestion patterns. The detected amplified length differences, RFLPs and the restriction sites can be considered as species specific markers for the allotetraploid cultivated cottons, which could be a useful tool for future studies of the cpDNA of the genus Gossypium L.

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Acknowledgements

This work was supported by a Grant-in-Aid (No 020518) from the Ministry of Education, Science, Sports and Culture of Japan for R. I. Cotton seeds were a gift from Nippon Shinyaku Co., LTD (Kyoto, Japan) to whom we send sincere gratitude. We would like to thank Dr. Abdelbagi Mukhtar, Gezira Research Station, Sudan, and Dr. Shirley Agrupis, Mariano Marcos State University, Philippines, for reading the manuscript.

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  1. Graduate School of Agriculture, Kyoto University, 606-8502, Kitashirakawa Oiwake-cho, Kyoto, Sakyu-Ku, Japan

    Rashid Ismael Hag Ibrahim, Jun-Ichi Azuma & Masahiro Sakamoto

  2. Department of Botany, Faculty of Science, University of Khartoum, P.O. Box 321, PC 11115, Khartoum, Sudan

    Rashid Ismael Hag Ibrahim

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  1. Rashid Ismael Hag Ibrahim
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Correspondence to Rashid Ismael Hag Ibrahim.

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Ibrahim, R.I.H., Azuma, JI. & Sakamoto, M. PCR-RFLP analysis of the whole chloroplast DNA from three cultivated species of cotton (Gossypium L.). Euphytica 156, 47–56 (2007). https://doi.org/10.1007/s10681-006-9348-4

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