, Volume 154, Issue 1–2, pp 249–254 | Cite as

The use of wheat/goatgrass introgression lines for the detection of gene(s) determining resistance to septoria tritici blotch (Mycosphaerella graminicola)

  • M. R. Simón
  • F. M. Ayala
  • C. A. Cordo
  • M. S. Röder
  • A. BörnerEmail author


At the IPK Gatersleben a series of 85 bread wheat (T. aestivum)/goatgrass (Aegilops tauschii) introgression lines was developed recently. Based on the knowledge that chromosome 7D of this particular Ae. tauschii is a donor of resistance to septoria tritici blotch (Mycosphaerella graminicola), a sub-set of thirteen chromosome 7D introgression lines was investigated along with the susceptible recipient variety ‘Chinese Spring’ (CS) and the resistant donor line ‘CS (Syn 7D)’. The material was inoculated with two Argentinian isolates of the pathogen (IPO 92067 and IPO 93014) at both the seedlings (two leaf) and adult (tillering) stages at two locations over 2 years (2003, 2004). The resistance was effective against both isolates and at both developmental stages, and the resistance locus maps to the centromeric region of chromosome arm 7DS. On the basis of its relationship with the microsatellite marker Xgwm44, it is likely that the gene involved is Stb5. Stb5 is therefore apparently effective against M. graminicola isolates originating from both Europe and South America.


Disease resistance Seedling stage Adult plant stage Septoria tritici blotch Mycosphaerella graminicola Aegilops tauschii Triticum aestivum Alien introgression 



The authors thank Winai Somprason, Ing. Marcelo Asborno, Ing. Martín Pardi and the staff of the Estación Experimental Facultad de Ciencias Agrarias, La Plata for their excellent technical assistance.


  1. Adhikari TB, Anderson JM, Goodwin SB (2003) Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola. Phytopathology 93:1158–1164PubMedGoogle Scholar
  2. Adhikari TB, Cavaletto JR, Dubcovsky J, Gieco JO, Schlatter AR, Goodwin SB (2004a) Molecular mapping of the Stb4 gene for resistance to Septoria tritici blotch in wheat. Phytopathology 94:1198–1206Google Scholar
  3. Adhikari TB, Wallwork H, Goodwin SB (2004b) Microsatellite markers linked to the Stb2 and Stb3 genes for resistance to Septoria tritici blotch in wheat. Crop Sci 44:1403–1411CrossRefGoogle Scholar
  4. Adhikari TB, Young X, Cavaletto JR, Hu X, Buechley G, Ohm HW, Shaner G, Goodwin SB (2004) Molecular mapping of Stb1, a potentially durable gene for resistance to septoria tritici blotch in wheat. Theor Appl Genet 109:944–953PubMedCrossRefGoogle Scholar
  5. Arraiano LS, Worland AJ, Ellerbrook C, Brown JKM (2001) Chromosomal location of a gene for resistance to Septoria tritici blotch (Mycosphaerella graminicola) in the hexaploid wheat Synthetic 6x. Theor Appl Genet 103:758–764CrossRefGoogle Scholar
  6. Brading PA, Verstappen ECP, Kema GHJ, Brown JKM (2002) A gene for gene relationship between wheat and Mycosphaerella graminicola, the Septoria tritici blotch pathogen. Phytopathology 92:439–445PubMedGoogle Scholar
  7. Chartrain L (2004) Genes for isolate-specific and partial resistance to septoria tritici blotch in wheat. PhD thesis, John Innes Centre, University of East Anglia, Norwich, UK, 151 pGoogle Scholar
  8. Chartrain L, Joaquin P, Berry ST, Arraiano LS, Azanza F, Brown JKM (2005) Genetics of resistance to septoria tritici blotch in the Portuguese wheat breeding line TE 9111. Theor Appl Genet 110:1138–1144PubMedCrossRefGoogle Scholar
  9. Eyal Z (1981) Integrated control of Septoria diseases of wheat. Plant Dis 65:763–768CrossRefGoogle Scholar
  10. Eyal, Z, Scharen AL, Prescott MJ, Van Ginkel M (1987) The septoria diseases of wheat: concepts and methods of disease management. Mexico, D.F., CIMMYT, Mexico, 46 pGoogle Scholar
  11. Kema GHJ, van Silfhout CH (1997) Genetic variation for virulence and resistance in the wheat-Mycosphaerella graminicola pathosystem III. Comparative seedling and adult plant experiments. Phytopathology 87:266–272PubMedGoogle Scholar
  12. McCartney CA, Brule-Babel AL, Lamari L, Somers DJ (2003) hromosomal location of a race specific resistance gene to Mycosphaerella graminicola in the spring cultivar ST6. Theor Appl Genet 107:1181–1186PubMedCrossRefGoogle Scholar
  13. McIntosh, RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat Scholar
  14. Pestsova EG, Börner A, Röder MS (2001) Development of a set of Triticum aestivum-Aegilops tauschii introgression lines. Hereditas 135:39–143CrossRefGoogle Scholar
  15. Pestsova EG, Börner A, Röder MS (2006) Development and QTL-assassment of Triticum aestivumAegilops tauschii introgression lines. Theor Appl Genet 112:634–647PubMedCrossRefGoogle Scholar
  16. Simón MR, Cordo CA (1999) Diallel analysis of incubation and latent period to Septoria tritici at two growth stages of wheat. J Genet Breed 53:73–78Google Scholar
  17. Simón MR, Worland AJ, Cordo CA, Struik PC (2001) Chromosomal location of resistance to Septoria tritici in seedlings of a synthetic hexaploid wheat, Triticum spelta and two cultivars of Triticum aestivum. Euphytica 109:151–155CrossRefGoogle Scholar
  18. Simón MR, Ayala FM, Cordo CA, Röder MS, Börner A (2004) Molecular mapping of quantitative trait loci determining resistance to septoria tritici blotch (Mycosphaerella graminicola) in wheat. Euphytica 138:41–48CrossRefGoogle Scholar
  19. Simón M.R., Perelló A, Cordo CA, Larrán S, van der Putten PEL, Struik PC (2005a) Association between Septoria tritici blotch, plant height, and heading date in wheat. Agron J 97:1072–1081CrossRefGoogle Scholar
  20. Simón MR, Worland AJ, Struik PC (2005b) Chromosomal location of genes encoding for resistance to septoria tritici blotch (Mycosphaerella graminicola) in substitution lines of wheat. Neth J Agr Sci 53:113–130Google Scholar
  21. Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • M. R. Simón
    • 1
  • F. M. Ayala
    • 1
  • C. A. Cordo
    • 2
  • M. S. Röder
    • 3
  • A. Börner
    • 3
    Email author
  1. 1.CerealiculturaUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.CIDEFI-CIC, Facultad de Ciencias Agrarias y ForestalesUniversidad Nacional de La PlataLa PlataArgentina
  3. 3.Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)GaterslebenGermany

Personalised recommendations