Advertisement

Euphytica

, Volume 154, Issue 1–2, pp 101–111 | Cite as

Quantitative trait loci for early maturity and their potential in breeding for earliness in Brassica juncea

  • Tariq MahmoodEmail author
  • Muhammad H. Rahman
  • Gary R. Stringam
  • Francis Yeh
  • Allen G. Good
Article

Abstract

A doubled haploid population of Brassica juncea, developed from a cross between two parental lines differing for days to maturity, was used to study the efficiency of indirect selection for a primary trait through selection of secondary trait(s) over direct selection for the primary trait when quantitative trait loci information is available for both primary and secondary traits, and applied. Days to maturity was considered as primary trait, while days to first flowering, days to end of flowering, flowering period and plant height were considered as secondary traits. An RFLP linkage map was employed for QTL analysis of maturity and maturity-determinant traits, and a stable QTL B6 simultaneously affecting these two types of traits was identified. This linked QTL explained 11.7% phenotypic variation for days to maturity, 20.7% variation for days to first flowering, 24.3% variation for days to end of flowering and 14.4% variation for plant height. Phenotypic evaluation of maturity and/or maturity-determinant traits, viz. days to first flowering, days to end of flowering and plant height revealed that limited genetic advance for early maturity can be achieved through phenotypic selection of the primary and/or the secondary trait(s). However, the estimates of genetic advance for early maturity based on combined phenotypic evaluation and linked QTL data was found to be, at least, three times higher compared to genetic advance based on phenotypic evaluation only, demonstrating the potential of marker-assisted selection in breeding for early maturity in B. juncea.

Keywords

Brassica juncea Maturity Flowering time Flowering period Plant height Quantitative trait loci Marker-assisted selection Indirect selection 

References

  1. Axelsson T, Brown CM, Sharp AG, Lydiate DJ, Langercrantz U (2000) Amphidiploid Brassica juncea contains conserved progenitor genomes. Genome 43:679–688PubMedCrossRefGoogle Scholar
  2. Axelsson T, Shavorskaya O, Lagercrantz U (2001) Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene. Genome 44:856–864CrossRefGoogle Scholar
  3. Camargo LEA, Osborn TC (1996) Mapping loci controlling flowering time in Brassica oleracea. Theor Appl Genet 92:610–616CrossRefGoogle Scholar
  4. Cheung WY, Friesen L, Rakow GFW, Seguin-Swartz G, Landry BS (1997) A RFLP-based linkage map of mustard [Brassica juncea (L.) Czern. and Coss.]. Theor Appl Genet 94:841–851CrossRefGoogle Scholar
  5. Cheung WY, Landry BS, Raney P, Rakow GFW (1998a) Molecular mapping of seed quality traits in Brassica juncea L. Czern. and Coss. Acta Hortic 459:139–147Google Scholar
  6. Cheung WY, Gugel RK, Landry BS (1998b) Identification of RFLP markers linked to the white rust resistance gene (Acr) in mustard (Brassica juncea (L.) Czern. and Coss.). Genome 41:626–628CrossRefGoogle Scholar
  7. Dudley JW (1993) Molecular markers in plant improvement: manipulation of genes affecting quantitative traits. Crop Sci 33:660–668CrossRefGoogle Scholar
  8. Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edn. Addison Wesley Longman Limited, EnglandGoogle Scholar
  9. Kwon SH, Torrie JH (1964) Heritability and interrelationship among traits of two soybean populations. Crop Sci 4:196–198CrossRefGoogle Scholar
  10. Lande R, Thompson R (1990) Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124:743–756PubMedGoogle Scholar
  11. Lionneton E, Ravera S, Sanchez L, Aubert G, Delourme R, Ochatt S (2002) Development of an AFLP-based linkage map and localization of QTLs for seed fatty acid content in condiment mustard (Brassica juncea). Genome 45:1203–1215PubMedCrossRefGoogle Scholar
  12. Lionneton E, Aubert G, Ochatt S, Merah O (2004) Genetic analysis of agronomic and quality traits in mustard (Brassica juncea). Theor Appl Genet 109:792–799PubMedCrossRefGoogle Scholar
  13. Mahmood T, Ekuere U, Yeh F, Good AG, Stringam GR (2003a) RFLP linkage analysis and mapping genes controlling the fatty acid profile of Brassica juncea using reciprocal DH populations. Theor Appl Genet 107:283–290CrossRefGoogle Scholar
  14. Mahmood T, Ekuere U, Yeh F, Good AG, Stringam GR (2003b) Molecular mapping of aliphatic glucosinoaltes in Brassica juncea. Genome 46:753–760CrossRefGoogle Scholar
  15. Mahmood T, Rahman MH, Stringam GR, Yeh F, Good AG (2005a) Molecular markers for yield components in Brassica juncea—do these assist in breeding for high seed yield. Euphytica 144:157–167CrossRefGoogle Scholar
  16. Mahmood T, Rahman MH, Stringam GR, Raney JP, Good AG (2005b) Molecular markers for seed colour in Brassica juncea. Genome 48:755–760CrossRefGoogle Scholar
  17. Mahmood T, Rahman MH, Stringam GR, Yeh F, Good AG (2006) Identification of quantitative trait loci (QTL) for oil and protein contents and their relationships with other seed quality traits in Brassica juncea. Theor Appl Genet 113:1211–1220PubMedCrossRefGoogle Scholar
  18. Negi MS, Devic M, Delseny M, Lakshmikumaran M (2002) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101:146–152CrossRefGoogle Scholar
  19. Rae AM, Howell EC, Kearsey MJ (1999) More QTL for flowering time revealed by substitution lines in Brassica oleracea. Heredity 83:586–596PubMedCrossRefGoogle Scholar
  20. SAS Institute Inc. (1989) SAS/STAT. SAS Institute Inc., CaryGoogle Scholar
  21. Schön CC, Melchinger AE, Boppenmaier J, Brunklaus-Jung E, Herrmann RG, Seitzer JF (1994) RFLP mapping in maize: quantitative trait loci affecting testcross performance of elite European flint lines. Crop Sci 34:378–389CrossRefGoogle Scholar
  22. Sharma R, Aggarwal RAK, Kumar R, Mohapatra T, Sharma RP (2002) Construction of an RAPD linkage map and localization of QTLs for oleic acid level using recombinant inbreds in mustard (Brassica juncea). Genome 45:467–472PubMedCrossRefGoogle Scholar
  23. Smith HF (1936) A discriminant function for plant selection. Ann Eugen 7:240–250Google Scholar
  24. Srivastava A, Gupta V, Pental D, Pradhan AK (2001) AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea. Theor Appl Genet 102:193–199CrossRefGoogle Scholar
  25. Teutonica RA, Osborn TC (1995) Mapping loci controlling vernalization requirement in Brassica rapa. Theor Appl Genet 91:1279–1283Google Scholar
  26. Thiagarajah MR, Stringam GR (1993) A comparison of genetic segregation in traditional and microspore-derived populations of Brassica juncea L. Czern and Coss. Plant Breed 111:330–334CrossRefGoogle Scholar
  27. Thurling N (1974) Morphophysiological determinants of yield in rapeseed (Brassica compestris and Brassica napus). I. Growth and morphological characters. Aust J Agric Res 25:697–710CrossRefGoogle Scholar
  28. Thurling N (1991) Application for ideotype concept in breeding for higher yield in the oilseed Brassicas. Field Crop Res 26:201–219CrossRefGoogle Scholar
  29. Van Ooijen JW, Maliepaard C (1996) MapQTL™ version 3.0: software for the calculation of QTL position on genetic maps. CRO-DLO, WageningenGoogle Scholar
  30. Veldboom LR, Lee M (1996) Genetic mapping of quantitative trait loci in maize in stress and non-stress environments: I. Grain yield and yield components. Crop Sci 36:1310–1319CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Tariq Mahmood
    • 1
    Email author
  • Muhammad H. Rahman
    • 1
  • Gary R. Stringam
    • 1
  • Francis Yeh
    • 2
  • Allen G. Good
    • 3
  1. 1.Department of Agricultural, Food and Nutritional ScienceUniversity of AlbertaEdmontonCanada
  2. 2.Department of Renewable ResourcesUniversity of AlbertaEdmontonCanada
  3. 3.Department of Biological SciencesUniversity of AlbertaEdmontonCanada

Personalised recommendations