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Insect-mediated seed-set evaluation of 21 soybean lines segregating for male sterility at 10 different loci

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Abstract

The first requirement to establish a successful hybrid soybean program is the availability of a stable male-sterile, female-fertile system. Male sterility has been an important tool in soybean breeding programs to improve traits such as yield, seed-protein and seed-oil content, and seed size. However, improvement of seed-set per se on male-sterile plants has not been an important breeding objective. The evaluation of the out-crossing potential of the available male-sterile, female-fertile soybean lines is crucial to determine the future of hybrid soybean. The objective of this study was to evaluate seed-set among 21 soybean lines segregating for male sterility at 10 different loci using Megachile rotundata as insect pollinator. Seed-set was evaluated in field conditions in 2001, 2002, and 2003 near Ames, Iowa. Our results indicated significant differences in seed-set among male-sterile lines. The effects of maturity group, pubescence color, the locus and/or the allele, and the genetic background of the lines segregating for male sterility were significant. Differences for seed-set among lines with independent mutational events at the same locus, (i.e. different alleles), also were significant. These results imply that interaction effects with the genetic background of the lines for traits related to fertility/sterility and insect-pollinator attraction and reward are important. Flower color had little effect on seed-set in the evaluated lines. The effect of year was very important on the performance of the lines for seed-set. This suggested that environmental conditions that favor plant–pollinator relationships need to be determined in order to increase insect-mediated cross-pollination to develop an efficient hybrid soybean program.

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Abbreviations

MG:

Maturity group

CV:

Coefficient of variation

LSD:

Least significant difference

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Acknowledgments

The authors thank Dairyland Seed Co., Inc, and Verde Seed Co., Inc. for partial support of this project.

Author information

Authors and Affiliations

  1. Department of Agronomy, Iowa State University, Ames, Iowa, 50011-1010, USA

    E. Ortiz-Perez

  2. Department of Genetics, Development and Cell Biology, and Bessey Microscopy Facility, Iowa State University, Ames, Iowa, 50011-1020, USA

    H. T. Horner

  3. USDA-ARS North Central Regional Plant Introduction Station, Iowa State University, Ames, Iowa, 50011-1010, USA

    S. J. Hanlin

  4. USDA-ARS CICGR, and Department of Agronomy, Iowa State University, Ames, Iowa, 50011-1010, USA

    R. G. Palmer

Authors
  1. E. Ortiz-Perez
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  2. H. T. Horner
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  3. S. J. Hanlin
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  4. R. G. Palmer
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Corresponding author

Correspondence to E. Ortiz-Perez.

Additional information

This is a joint contribution from the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 3769, and from the USDA, Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, and supported by the Hatch Act and the State of Iowa. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by Iowa State University or the USDA, and the use of the name by Iowa State University or the USDA imply no approval of the product to the exclusion of others that may also be suitable.

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Ortiz-Perez, E., Horner, H.T., Hanlin, S.J. et al. Insect-mediated seed-set evaluation of 21 soybean lines segregating for male sterility at 10 different loci. Euphytica 152, 351–360 (2006). https://doi.org/10.1007/s10681-006-9222-4

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  • DOI: https://doi.org/10.1007/s10681-006-9222-4

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