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Molecular Analysis of Local Potato Cultivars from Tenerife Island Using Microsatellite Markers

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Abstract

We have used 19 SSR markers to fingerprint 41 local potato cultivars from 10 locations of Tenerife Island. These varieties represent relicts of the early introductions originating from South America and have been characterised previously morphologically and ecophysiologically. The SSR primers generated a varying degree of polymorphisms. A total of 67 alleles were observed, 12 of them were present in all cultivars. Several accession and group specific alleles were detected. Similarity coefficients were computed from the molecular data and cluster analyses were performed. Generally, cultivar groups with identical or related common names showed the same SSR patterns or clustered closely together. According to the molecular patterns misleading or confounded names were evident for four accessions. The dendrogram clusters were generally in good agreement with previous classifications of the accessions as Solanum tuberosum subsp. andigena, S. tuberosum subsp. tuberosum and Solanum chaucha genotypes. However in four cases the molecular patterns showed discrepancies with previous species assignments suggesting the need for a more detailed and comparative study of these accessions.

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Acknowledgements

This work was financed in the frame of the INIA project RF03-004 and by the Department of Agriculture and Fisheries of the Basque Government.

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Correspondence to J. I. Ruiz de Galarreta.

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Barandalla, L., de Galarreta, J.I.R., Rios, D. et al. Molecular Analysis of Local Potato Cultivars from Tenerife Island Using Microsatellite Markers. Euphytica 152, 283–291 (2006). https://doi.org/10.1007/s10681-006-9215-3

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