Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice

Abstract

We have established marker-aided selection strategies for the two major Rf genes (Rf3 and Rf4) governing fertility restoration of␣cytoplasmic-genetic male sterility (CMS) in rice. Polymorphisms between restorer and non-restorer␣lines were observed using RG140/PvuII for Rf3 located on chromosome 1 and S10019/BstUI for Rf4 located on chromosome 10. DNA polymorphisms associated with these two loci in restorer lines of wild abortive (WA), Dissi, and Gambiaca cytoplasm are conserved, suggesting that similar biological processes control pollen fertility in this diverse cytoplasm. Because of their close linkage to Rf genes and distinct banding patterns, STS markers RG140/PvuII and S10019/BstUI are well suited for marker-aided selection, enhanced backcross procedures, and pyramiding of Rf genes in agronomically superior non-restorer lines. The combined use of markers associated with these two loci improved the efficiency of screening for putative restorer lines from a set of elite lines. Positional analyses of Rf4 and the inheritance pattern of the polymorphism in S10019/BstUI suggest that Rf4, governing fertility restoration in WA-CMS in rice, is likely to be the same gene governing fertility restoration in BT- and HL-CMS that has a gametophytic effect, which explains why 100% pollen fertility in hybrids is impossible to attain.

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References

  1. Bharaj TS, Bains SS, Sidhu GS, Gagneja MR (1991) Genetics of fertility restoration of ‘wild abortive’ cytoplasmic male sterility in rice, Oryza sativa L. Euphytica 56:199–203

    Article  Google Scholar 

  2. Bharaj TS, Virmani SS, Khush GS (1995) Chromosomal location of fertility restoring genes for ‘wild abortive’ cytoplasmic male sterility using primary trisomics in rice. Euphytica 83:169–173

    Article  Google Scholar 

  3. Ellis JG, Lawrence GJ, Finnegan EJ, Anderson PA (1995) Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci USA 92:4185–4188

    PubMed  Article  CAS  Google Scholar 

  4. Govinda Raj K, Virmani SS (1988) Genetics of fertility restoration of ‘WA’ type cytoplasmic male sterility in rice. Crop Sci 28:787–792

    Article  Google Scholar 

  5. Grog R, Hollricher K, Schulzelefert P (1993) Functional analysis and RFLP mediated mapping of the Mlg resistance locus in barley. Plant J 3:846–857

    Google Scholar 

  6. Huang QY, He YQ, Jing RC, Zhu RS, Zhu YG (2000) Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers. Chinese Sci Bull 45:430–432

    CAS  Article  Google Scholar 

  7. Huang J, Hu J, Xu X, Li S, Yi P, Yang D, Ren F, Liu X, Zhu Y (2003) Fine mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice. Bot Bull Acad Sin 44:285–289

    CAS  Google Scholar 

  8. Ichikawa N, Kishimoto N, Inagaki A, Nakamura A, Koshino Y, Yokozecki Y, Oka HI, Samoto S, Akagi H, Higo K, Shinjyo C, Fujmura T, Shimada H (1997) A rapid PCR-based selection of a rice line containing the Rf-1 gene, which is involved in restoration of the cytoplasmic male sterility. Mol Breed 3:195–202

    Article  CAS  Google Scholar 

  9. Jing R, Li X, Yi P, Zhu Y (2001) Mapping fertility restoring genes of rice ‘WA’ cytopasmic male sterility using SSLP markers. Bot Bull Acad Sin 42:167–171

    CAS  Google Scholar 

  10. Komori T, Yamamoto T, Takemori N, Kashihara M, Matsushima H, Nitta N (2003) Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers. Euphytica 129:241–247

    Article  CAS  Google Scholar 

  11. Li YC, Yuan LP (1986) Genetic analysis of fertility restoration in male sterile lines of rice. In: IRRI (eds) Rice genetics. International Rice Research Institute, Manila, Philippines, pp 617–632

  12. Li Z, Shao WH, Zhu YK, Li RJ, Liu ZL, Wan JM (1982) The study and practice of hybrid rice. Shanghai press, Shanghai, China

    Google Scholar 

  13. Lin SC, Yuan LP (1980) Hybrid rice breeding in China. In: IRRI (eds) Innovative approaches to rice breeding. International Rice Research Institute, Manila, Philippines, pp 35–51

  14. McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghiran R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Jellstrom RF, Declerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice, Oryza sativa L. DNA Res 9:199–207

    PubMed  Article  CAS  Google Scholar 

  15. Nas TMS, Casal CL, Li Z, Virmani SS (2003) Application of molecular markers for identification of restorers. Rice Genet Newslett 20:69–71

    Google Scholar 

  16. Sattari M (2004) Genetic relationship, molecular mapping, and development of marker-assisted selection for fertility restoration (Rf) genes for rice cytoplasmic male sterility systems. PhD Thesis, University of the Philippines at Los Baños, Philippines

  17. Shen Y, Cai Q, Gao M, Wang X (1996) Isolation and genetic characterization of fertility restoring revertant induced from cytoplasmic male sterile rice. Euphytica 90:17–23

    Google Scholar 

  18. Shinjyo C (1975) Genetical studies of cytoplasmic male sterility and fertility restoration in rice, Oryza sativa L. Sci Bull Coll Agric Univ Ryukyus 22:1–57

    Google Scholar 

  19. Tan XL, Vanavichit A, Amornsilpa S, Trangoonrung S (1998) Genetic analysis of rice CMS-WA fertility restoration based on QTL mapping. Theor Appl Genet 96:994–999

    Article  Google Scholar 

  20. Teng LS, Shen ZT (1994) Inheritance of fertility restoration for cytoplasmic male sterility in rice. Rice Genet Newslett 11:95–97

    Google Scholar 

  21. Virmani SS (2003) Advances in hybrid rice research and development in the tropics. In: Virmani SS, Mao CX, Hardy B (eds) Hybrid rice for food security, poverty alleviation, and environmental protection. Proceedings of the 4th International Symposium on Hybrid Rice, 14–17 May 2002, Hanoi, Vietnam. International Rice Research Institute, Manila, Philippines, pp 7–20

    Google Scholar 

  22. Virmani SS, Wan BH (1988) Development of CMS lines in hybrid rice breeding. In: IRRI (eds) Hybrid rice. International Rice Research Institute, Manila, Philippines, pp 103–114

  23. Virmani SS, Govinda Raj K, Casal CL, Dalmacio RD, Aurin PA (1986) Current knowledge and future outlook on cytoplasmic genetic male sterility and fertility restoration in rice. In: IRRI (eds) Rice genetics. International Rice Research Institute, Manila, Philippines, pp 633–647

  24. Yao FY, Xu CG, Yu SB, Li JX, Gao YJ, Li XH, Zhang Q (1997) Mapping and genetic analysis of two fertility restorer loci in the ‘wild abortive’ cytoplasmic male sterility system of rice, Oryza sativa L. Euphytica 98:183–187

    Article  CAS  Google Scholar 

  25. Young JB, Virmani SS (1984) Inheritance of fertility restoration in a rice cross. Rice Genet Newslett 1:102–103

    Google Scholar 

  26. Yuan LP (1998) Hybrid rice breeding in China. In: Virmani SS, Siddiq EA, Muralidharan K (eds) Advances in hybrid rice technology. Proceedings of the 3rd international symposium on hybrid rice, 14–16 November 1996, Hyderabad, India. International Rice Research Institute, Manila, Philippines, pp 27–33

    Google Scholar 

  27. Zhang G, Bharaj TS, Lu Y, Virmani SS, Huang N (1997) Mapping of the Rf-3 nuclear fertility-restoring gene for ‘WA’ cytoplasmic male sterility in rice using RAPD and RFLP markers. Theor Appl Genet 94:27–33

    Article  CAS  PubMed  Google Scholar 

  28. Zhang QY, Liu YG, Mei MT (2002) Molecular mapping of the fertility restorer gene Rf4 for WA cytoplasmic male sterility. Acta Genet Sin 29:1001–1004

    PubMed  Google Scholar 

  29. Zheng KL, Huang N, Bennett J, Khush GS (1995) PCR-based marker-assisted selection in rice breeding. IRRI Discuss. Pap. Ser. 12. International Rice Research Institute, Manila, Philippines

    Google Scholar 

  30. Zhou T (1983) Analysis of R genes in hybrid indica rice of ‘WA’ type. Acta Agron Sin 9:241–247

    Google Scholar 

  31. Zhu YG (2000) Studies on rice male sterility and its restoration. In: Zhu YG, Li YQ, Wang MQ (eds) Biology of male sterility in rice. Wuhan Univ. Press, Wuhan, China

    Google Scholar 

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Acknowledgements

This research was kindly supported by a graduate program scholarship from IRRI and the Ministry of Agriculture of the Islamic Republic of Iran.

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Correspondence to Sant S. Virmani.

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Sattari, M., Kathiresan, A., Gregorio, G.B. et al. Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice. Euphytica 153, 35–42 (2007). https://doi.org/10.1007/s10681-006-9213-5

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Keywords

  • CMS
  • Fertility restorer gene
  • Hybrid rice
  • Marker-aided selection
  • Rf3
  • Rf4
  • Wild abortive