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Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice

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Abstract

We have established marker-aided selection strategies for the two major Rf genes (Rf3 and Rf4) governing fertility restoration of␣cytoplasmic-genetic male sterility (CMS) in rice. Polymorphisms between restorer and non-restorer␣lines were observed using RG140/PvuII for Rf3 located on chromosome 1 and S10019/BstUI for Rf4 located on chromosome 10. DNA polymorphisms associated with these two loci in restorer lines of wild abortive (WA), Dissi, and Gambiaca cytoplasm are conserved, suggesting that similar biological processes control pollen fertility in this diverse cytoplasm. Because of their close linkage to Rf genes and distinct banding patterns, STS markers RG140/PvuII and S10019/BstUI are well suited for marker-aided selection, enhanced backcross procedures, and pyramiding of Rf genes in agronomically superior non-restorer lines. The combined use of markers associated with these two loci improved the efficiency of screening for putative restorer lines from a set of elite lines. Positional analyses of Rf4 and the inheritance pattern of the polymorphism in S10019/BstUI suggest that Rf4, governing fertility restoration in WA-CMS in rice, is likely to be the same gene governing fertility restoration in BT- and HL-CMS that has a gametophytic effect, which explains why 100% pollen fertility in hybrids is impossible to attain.

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Acknowledgements

This research was kindly supported by a graduate program scholarship from IRRI and the Ministry of Agriculture of the Islamic Republic of Iran.

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  1. Majid Sattari

    Present address: Rice Research Institute of Iran, P.O. Box 13475, Rasht, Iran

Authors and Affiliations

  1. Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines

    Majid Sattari, Arumugam Kathiresan, Glenn B. Gregorio, Tamerlane M. Nas & Sant S. Virmani

  2. Department of Agronomy, University of the Philippines at Los Baños, 4031, Laguna, Philippines

    Jose E. Hernandez

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  1. Majid Sattari
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  2. Arumugam Kathiresan
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  5. Tamerlane M. Nas
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  6. Sant S. Virmani
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Correspondence to Sant S. Virmani.

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Sattari, M., Kathiresan, A., Gregorio, G.B. et al. Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice. Euphytica 153, 35–42 (2007). https://doi.org/10.1007/s10681-006-9213-5

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  • DOI: https://doi.org/10.1007/s10681-006-9213-5

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