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Euphytica

, Volume 151, Issue 3, pp 401–409 | Cite as

AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.)

  • Dengfeng Hong
  • Lili Wan
  • Pingwu Liu
  • Guangsheng YangEmail author
  • Qingbiao He
Original Article

Abstract

Rs1046AB is a line which is true breeding for a dominant genetic male sterility gene (Ms) but which is a mixture of male fertile and sterile individuals (a two-type line) because it is segregating for a dominant suppressor gene (Rf). This system provides a promising alternative to the CMS system for hybrid breeding in Brassica napus. In order to identify molecular markers linked to the rf gene, a near-isogenic line (NIL) population from the cross between a sterile individual (MsMsrfrf) and a fertile individual (MsMsRfrf) in Rs1046AB was subjected to amplified fragment length polymorphism (AFLP) analysis, with a combination of comparing near isogenic lines (NILs) and bulked segregant analysis (BSA). From 2,816 pairs of AFLP primers, six fragments showing polymorphism between the fertile and sterile bulks as well as the individuals of the bulks were identified. Linkage analysis indicated that the six AFLP markers are tightly linked to the Rf gene and all are distributed on the same side. The minimum genetic distance between the Rf gene and a marker was 0.7 cM. Since the AFLP markers are not suitable for large-scale application in MAS (marker-assisted selection), our objective was to develop a fast, cheap and reliable PCR-based assay. Consequently, three of the four closest AFLP markers were converted directly to sequence characterized amplified region (SCAR) markers. For the other marker a corresponding SCAR marker was successfully obtained after isolating the adjacent sequences by PCR Walking. The available SCAR markers of the Rf gene will greatly facilitate future breeding programs using dominant GMS to produce hybrid varieties.

Keywords

Brassica napus Genetic male sterility Suppressor gene AFLP SCAR MAS 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Dengfeng Hong
    • 1
  • Lili Wan
    • 1
  • Pingwu Liu
    • 1
  • Guangsheng Yang
    • 1
    Email author
  • Qingbiao He
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement (Wuhan Branch)Huazhong Agricultural University WuhanWuhanChina

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