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Euphytica

, Volume 151, Issue 3, pp 393–400 | Cite as

Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion

  • Yuqiang Sun
  • Yichun Nie
  • Xiaoping Guo
  • Chao Huang
  • Xianlong ZhangEmail author
Article

Abstract

Somatic hybrids were obtained from electrofused protoplasts derived from embryogenic suspension cultures of tetraploid cotton (G. hirsutum L. cv. Coker 201) and embryogenic callus of diploid wild cotton G. davidsonii. The regenerants were initially identified as hybrids by RAPD (random amplified polymorphic DNA) analysis. Subsequently, observation on chromosome counting, morphology and SSR (simple sequence repeat) confirmed the hybrid status. Cytological investigation of the metaphase root-tip cells of the regenerated plants revealed there were 74 to 84 chromosomes in the plants, close to the expected 78 chromosomes. SSR analysis revealed the regenerated plants contained specific genomic fragments from both fusion partners, further confirmed their hybridity. The morphology of the plants was intermediate between the two fusion partners. The regenerants were difficult to develop into mature plants because their roots browned and they wilted from the stem apex before forming 3 to 5 true leaves. The hybrid plants were transferred to soil by grafting in vitro onto rootstocks.

Keywords

Gossypium hirsutum G. davidsonii Protoplast fusion Somatic hybrid RAPD SSR 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Yuqiang Sun
    • 1
  • Yichun Nie
    • 1
  • Xiaoping Guo
    • 1
  • Chao Huang
    • 1
  • Xianlong Zhang
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanP. R. China

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