We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus.
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amplified fragment length polymorphism
cleaved amplified polymorphic sequence
cytoplasmic male sterility
marker assisted selection
polyacrylamide gels electrophoresis
polymerase chain reaction
random amplified polymorphic DNA
sequence characterized amplified region
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Liu, Z., Fu, T., Wang, Y. et al. Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L. Euphytica 149, 381–385 (2006). https://doi.org/10.1007/s10681-006-9084-9
- Molecular Marker
- Yellow Seed Coat