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Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L

Summary

We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus.

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Abbreviations

AFLP:

amplified fragment length polymorphism

CAPS:

cleaved amplified polymorphic sequence

CM:

centiMorgan

CMS:

cytoplasmic male sterility

DH:

doubled haploid

EB:

ethidium bromide

MAS:

marker assisted selection

PAGE:

polyacrylamide gels electrophoresis

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

SCAR:

sequence characterized amplified region

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Correspondence to Tingdong Fu.

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Liu, Z., Fu, T., Wang, Y. et al. Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L. Euphytica 149, 381–385 (2006). https://doi.org/10.1007/s10681-006-9084-9

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Key words

  • CAPS
  • MAS
  • Molecular Marker
  • SCAR
  • Yellow Seed Coat