Genetic Divergence and Molecular Characterization of Sorghum Hybrids and their Parents for Reaction to Atherigona Soccata (Rondani)

Summary

Simple sequence repeat (SSR) markers linked to quantitative trait loci (QTL) associated with resistance to sorghum shoot fly, Atherigona soccata resistance were used to characterize the genetic and phenotypic diversity of 12 cytoplasmic male-sterile (CMS) and maintainers, 12 restorer lines, and 144 F1 hybrids. The genetic diversity was quite high among the shoot fly-susceptible parents and the hybrids based on them, as indicated by high polymorphic information content (PIC) values, while limited genetic diversity was observed among shoot fly-resistant lines. The phenotypic and genotypic dissimilarity analysis indicated that the shoot fly-resistant and -susceptible parents were 73.2 and 38.5% distinct from each other, and the morphological and genetic distances of certain resistant and susceptible cross combinations was more than their resistant or susceptible parents. Genetic variability among the groups was low (10.8%), but high within groups (89.2%). The genetic and morphological distances suggested that the F1 hybrids were closer to CMS (5 to 12% dissimilar) than the restorer (11 to 87% dissimilar), suggesting that CMS influences the expression of resistance to sorghum shoot fly. The SSR markers can be used to characterize the homologous traits in sorghum germplasm.

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References

  1. Agrama, H.A., G.E. Widle, J.C. Reese, L.R. Campbell & M.R. Tuinstra, 2002. Genetic mapping of QTLs associated with green bug resistance and tolerance in Sorghum bicolor. Theor Appl Genet 104: 1373–1378.

    PubMed  CAS  Article  Google Scholar 

  2. Bhattramakki, D., J. Dong, A.K. Chhabra & G.E. Hart, 2000. An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43: 988–1002.

    CAS  Article  Google Scholar 

  3. Brown, S.M., S.M. Hopkins, S.E. Mitchell, M. Senior, T.Y. Wang, R.R. Duncan, F. Gonzalez-Candelas & S. Kesovichsel, 1996. Multiple methods for true identification of polymorphic simple sequence repeats (SSR) in sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 93: 190–198.

    CAS  Article  Google Scholar 

  4. Dawson, I.K., K.J. Chalmers, R. Waugh & W. Powell, 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers. Mol Ecol 2: 151–159.

    PubMed  CAS  Google Scholar 

  5. Dean, R.E., J.A. Dahlberg, M.S. Hopkins, S.E. Mitchell & S. Kresovich, 1999. Genetic redundancy and diversity among ‘Orange’ accessions in the US national sorghum collection as assessed with simple sequence repeat (SSR) markers. Crop Sci 39: 1215–1221.

    Article  Google Scholar 

  6. Deu, M.D. Gonzalez-de-leon, J.C. Glaszmann, J. Degremont, I. Chantereau, C. Lanaud & P. Hamon, 1994. RFLP diversity in cultivated sorghum in relation to racial differentiation. Theor Appl Genet 88: 838–844.

    Article  Google Scholar 

  7. Dhillon, M.K., 2004. Effects of cytoplasmic male-sterility on expression of resistance to sorghum shoot fly, Atherigona soccata (Rondani). Ph.D. Thesis, 382 pp. Department of Entomology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India.

  8. Dice, L.R., 1945. Measures of the amount of ecological association between species. Ecol. 26: 97–302.

    Article  Google Scholar 

  9. Dje, Y., M. Heuertz, C. Lefebvre & X. Vekemans, 2000. Assessment of genetic diversity within and among germplasm accessions in cultivated sorghum using microsatellite markers. Theor Appl Genet 100: 918–925.

    CAS  Article  Google Scholar 

  10. Ejeta, G., M.R. Goldsbrough, E.M. Tunistra, A. Grote, Y. Menkir, Y. Ibrahim, N. Cisse, Y. Weerasuriya, A. Melake-Berhan & C.A. Shaner, 2000. Molecular marker application in sorghum. In: B.I.G. Haussmann, H.H. Geiger, D.E. Hess, C.T. Hash & P. Bramel-Cox (Eds.), Application of molecular markers in plant breeding, Training manual for a seminar held at IITA, Ibadan, Nigeria, 16–17 August 1999, pp. 81–89. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India.

  11. Folkertsma, R.T., G.M. Sajjanar, B.V.S. Reddy, H.C. Sharma & C.T. Hash, 2003. Genetic mapping of QTL associated with sorghum shoot fly (Atherigona soccata) resistance in sorghum (Sorghum bicolor). In: Final Abstracts Guide, Plant and Animal Genome XI, 11–15 January 2003, p. 462. Town & Country Hotel, San Diego, CA, USA (http://www.intl-pag.org/11/abstracts/P5d_P462_XI.html).

  12. Fritz, A.K., S. Caldwell & W.D. Worrall, 1999. Molecular mapping of Russian wheat aphid resistance from triticale accession PI 386156. Crop Sci 39: 1707–1710.

    CAS  Article  Google Scholar 

  13. Ghebru, B., R.J. Schmidt & J.L. Bennetzen, 2002. Genetic diversity of Eritrean sorghum landraces assessed with simple sequence repeat (SSR) markers. Theor Appl Genet 105: 229–236.

    PubMed  CAS  Article  Google Scholar 

  14. Gowda, P.S.B., G.W. Xu, R.A. Frederiksen & C.W. Magill, 1995. DNA markers for downy mildew resistance genes in sorghum. Genome 38: 823–826.

    CAS  PubMed  Google Scholar 

  15. Grenier, C., M. Deu, S. Kresovich, P.J. Bramel-Cox & P. Hamon, 2000. Assessment of genetic diversity in three subsets constituted from the ICRISAT sorghum collection using randon vs. non-random sampling procedures. B. Using molecular markers. Theor Appl Genet 101: 197–202.

    CAS  Article  Google Scholar 

  16. Hash, C.T., R.T. Folkertsma, P. Ramu, B.V.S. Reddy, V. Mahalakshmi, H.C. Sharma, H.F.W. Rattunde, E.R. Weltzien, B.I.G. Haussmann, M.E. Ferguson & J.H. Crouch, 2003. Marker-assisted breeding across ICRISAT for terminal drought tolerance and resistance to shoot fly and Striga in sorghum. In: Abstracts, In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution, 27–31 May 2002, p. 81. University of Bologna, Bologna, Italy.

  17. House, L.R., 1985. A Guide to Sorghum Breeding. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India, pp. 83–84.

  18. Huff, D.R., R. Peakell & P.E. Smouse, 1993. RAPD variation within and between natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.). Theor Appl. Genet 86: 927–934.

    CAS  Article  Google Scholar 

  19. ICRISAT, 1992. The Medium Term Plan. Volume II. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India.

  20. Jotwani, M.G., W.R. Young & G.L. Teetes, 1980. Elements of integrated control of sorghum pests. FAO Plant Production and Protection Paper. Food and Agriculture Organization, Rome, Italy, 159 p.

  21. Kong, L., J. Dong & G.E. Hart, 2000. Characteristics, linkage-map positions, and allelic differentiation of Sorghum bicolor (L.) Moench DNA simple sequence repeats (SSRs). Theor Appl Genet 101: 438–448.

    CAS  Article  Google Scholar 

  22. Mace, E.S., H.K. Buhariwalla & J.H. Crouch, 2003. A high throughput DNA extraction protocol for tropical molecular breeding programs. Pl Mol Biol Reporter 21: 459a–459h.

    Google Scholar 

  23. Menkir, A., P. Goldsbrough & G. Ejeta, 1997. RAPD based assessment of genetic diversity in cultivated races of sorghum. Crop Sci 37: 564–569.

    CAS  Article  Google Scholar 

  24. Menz, M.A., R.R. Klein, N.C. Unruh, W.L. Rooney, P.E. Klein & J.E. Mullet, 2004. Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers. Crop Sci 44: 1236–1244.

    CAS  Article  Google Scholar 

  25. Morgante, M. & A.M. Olivieri, 1993. PCR amplified microsatellites as markers in plant genetics. Plant J 3: 175–182.

    PubMed  CAS  Article  Google Scholar 

  26. Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet 89: 583–590.

    CAS  Google Scholar 

  27. Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76: 5269–5273.

    PubMed  CAS  Article  Google Scholar 

  28. Oliviera, A.C. de, T. Richter & J.L. Bennetzen, 1996. Regional and racial specificities in sorghum germplasm assessed with DNA Markers. Genome 39: 579–587.

    Google Scholar 

  29. Paterson, A.H., Y. Lin, Z. Li, K.F. Schertz, J.F. Doebley, S.R.M. Pinson, S. Lin, J.W. Stanzel & J.E. Irvine, 1995. Convergent domestication of cereal crops by independent mutations at corresponding genetic loci. Sci 269: 1714–1718.

    CAS  Google Scholar 

  30. Pereira, M.G. & M. Lee, 1995. Identification of genomic regions affecting plant height in sorghum and maize. Theor Appl Genet 90: 380–388.

    CAS  Article  Google Scholar 

  31. Reif, J.C., A.E. Melchinger, X.C. Xia, M.L. Warburton, D.A. Hoisington, S.K. Vasal, G. Srinivasan, M. Bohn & M. Frisch, 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize populations. Crop Sci 43: 1275–1282.

    Article  Google Scholar 

  32. Rohlf, J.F., 2000. Applied Biostastics, Inc. Department of Ecology and Evolution, State University of New York, Stone Brook, New York 11794-52-45, USA.

  33. Rongwen, J., M.S. Akkaya, A. Bhagwar, U. Lavi & P.B. Cregan, 1995. The use of microsatellite DNA markers for Soybean genotype identification. Theor Appl Genet 90: 43–48.

    CAS  Article  Google Scholar 

  34. Sajjanar, G.M., 2002. Genetic analysis and molecular mapping of components of resistance to shoot fly (Atherigona soccata Rond.) in sorghum [Sorghum bicolor (L.) Moench]. Ph.D. Thesis, pp. 265. Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad 500005, Karnataka, India.

  35. Sharma, H.C., 1993. Host plant resistance to insects in sorghum and its role in integrated pest management. Crop Prot 12: 11–34.

    Article  Google Scholar 

  36. Sharma, H.C., M.K. Dhillon, J.S. Naresh, Ram Singh, G. Pampapathy & B.V.S. Reddy, 2004. Influence of cytoplasmic male-sterility on the expression of resistance to insects in sorghum. In: T. Fisher, N. Turner, J. Angus, L. McIntyre, M. Robertson, A. Burrell & D. Llyod (Eds.), Fourth International Crop Science Congress, 25 Sept.–2 Oct. 2004, p. 6. Brisbane, Queensland, Australia (http://www.cropscience.org.au)

  37. Sharma, H.C. & K.F. Nwanze, 1997. Mechanisms of resistance to insects and their usefulness in sorghum improvement. Information Bulletin No. 45, 47 pp. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India.

  38. Sharma, H.C., S.L. Taneja, N. Kameswara Rao & K.E. Prasada Rao, 2003. Evaluation of sorghum germplasm for resistance to insect pests. Information Bulletin No. 63, 177 pp. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India.

  39. Sharma, H.C., S.L. Taneja, K. Leuschner & K.F. Nwanze, 1992. Techniques to screen sorghum for resistance to insect pests. Information Bulletin No. 32, 48 pp. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Andhra Pradesh, India.

  40. Smith, J.S.C., S. Kresovich, M.S. Hopkins, S.E. Mitchell, R.E. Dean, W.L. Woodman, M. Lee & K. Porter, 2000. Genetic diversity among elite sorghum inbred lines assessed with simple sequence repeats. Crop Sci 40: 226–232.

    CAS  Article  Google Scholar 

  41. Stephens, J.C. & R.F. Holland, 1954. Cytoplasmic male sterility for sorghum seed production. Agron J 46: 20–23.

    Article  Google Scholar 

  42. Tao, Y.Z., R.G. Henzell, D.R. Jordan, D.G. Butler, A.M. Kelly & C.L. McIntyre, 2000. Identification of genetic regions associated with stay green in sorghum by testing RILs in multiple environments. Theor Appl Genet 100: 1225–1232.

    CAS  Article  Google Scholar 

  43. Tao, Y.Z., D.R. Jordan, R.G. Henzell & C.L. McIntyre, 1998. Construction of an integrated sorghum genetic map in a sorghum RIL population by using probes from different sources and its alignment with other sorghum maps. Australian J Agric Res 49: 729–736.

    CAS  Article  Google Scholar 

  44. Taramino, G., R. Tarchini, S. Ferrario, M. Lee & M.E. Pe, 1997. Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor. Theor Appl Genet 95: 66–72.

    CAS  Article  Google Scholar 

  45. Tuinstra, M.R., G. Ejeta & P.B. Goldsbough, 1998. Evaluation of near-isogenic sorghum lines contrasting for QTL markers associated with drought tolerance. Crop Sci 38: 835–842.

    Article  Google Scholar 

  46. Wang, Z., J.L. Weber, G.T. Zhong & S.D. Tanskley, 1994. Survey of plant short tandem DNA repeats. Theor Appl Genet 88: 1–6.

    CAS  Google Scholar 

  47. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. Oligonucleotide primers of arbitrary sequence amplify DNA polymorphisms which are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.

    PubMed  CAS  Google Scholar 

  48. Witcombe, J.R. & C.T. Hash, 2000. Resistance deployment strategies in cereal hybrids using marker-assisted selection: Gene pyramiding, three-way hybrids, and synthetic parent populations. Euphytica 112: 175–186.

    Article  Google Scholar 

  49. Yang, G.K., M.A. Saghai Maroof, C.G. Xu, Q. Zhang & R.M. Biyashev, 1995. Comparative analysis of microsatellite DNA polymorphism in land races and cultivars of rice. Mol Gen Genet 245: 187–194.

    Google Scholar 

  50. Yang, R.C., H.R. Lu, X.B. Zhang, Y.H. Xia, W.M. Li, K.J. Liang, N.Y. Wang & Q.H. Chen, 1989. Study of the susceptibility of CMS WA cytoplasm in rice to blast and bacterial blight. Acta Agron Sinica 15: 310–318.

    Google Scholar 

  51. Yang, W., A.C. de Oliviera, I. Godwin, K. Schertz & J.L. Bennetzen, 1996. Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums. Crop Sci 36: 1669–1676.

    Article  Google Scholar 

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Dhillon, M.K., Sharma, H.C., Folkertsma, R.T. et al. Genetic Divergence and Molecular Characterization of Sorghum Hybrids and their Parents for Reaction to Atherigona Soccata (Rondani). Euphytica 149, 199–210 (2006). https://doi.org/10.1007/s10681-005-9067-2

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Key words

  • Sorghum shoot fly
  • Atherigona soccata
  • SSR markers
  • sorghum
  • resistance
  • cytoplasmic male-sterility