Interspecific hybridization is an effective method to generate a new crop that gains available functions in a short time. Interspecific hybrids (2n= 16) betweenAllium fistulosumL. (2n= 16) andAllium schoenoprasumL. (2n= 16) were produced by reciprocal crossings through ovary culture, but the hybrids were much fewer in the combination usingA. schoenoprasumas a seed plant. All the hybrids have eight long chromosomes originated fromA.fistulosumand eight short chromosomes originated fromA. schoenoprasum. In addition, the hybridity was confirmed by randomly amplified polymorphic DNA (RAPD) analysis and cleaved amplified polymorphic sequence (CAPS) analysis of the rDNA internal transcribed spacer (ITS) region. The interspecific hybrids showed a vigorous growth habit; their foliage was slightly bloomy and deep green. The hybrids did not form bulbs, but rather propagated vegetatively by tillering. Carotene contents of the hybrids and both parents were quantified using high-performance liquid chromatography (HPLC). The contents of all edible parts of the hybrids were approximately seven times higher than those of either parent. These results indicate that the hybrid is a new and carotene-rich vegetable ofAlliumspecies.
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Amagi, M., K. Ohashi, S. Kimura, N. Oguri & A. Kojima, 1995. Breeding of an interspecies hybrid between Allium fistulosum and A. tuberosum by embryo culture. Bull Tochigi Agr Exp Stn 43: 87–94. (In Japanese)
Baldwin, B.G., 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. Mol Phylog Evol 1: 3–16.
Buiteveld, J., Y. Suo, M.M. van Lookeren Campagne & J. Creemers-Molenaar, 1998. Production and characterization of somatic hybrid plants between leek (Allium ampeloprasum L.) and onion (Allium cepa L.). Theor Appl Genet 96: 765–775.
Dunstan, D.I. & K.C. Short, 1977. Improved growth of tissue cultures of the onion, Allium cepa. Physiol Plant 41: 70–72.
Gonzalez, L.G. & B.V. Ford-Lloyd, 1987. Facilitation of wide-crossing through embryo rescue and pollen storage in interspecific hybridization of cultivated Allium species. Plant Breed 98: 318–322.
Greenwood, M.S., C.A. Hopper & K.W. Hutchison, 1989. Maturation in larch. I. Effect of age on shoot growth, foliar characteristics, and DNA methylation. Plant Physiol 90: 406–412.
Hanelt, P., 2001. Alliaceae. In: P. Hanelt (Ed.), Mansfeld's Encyclopedia of Agricultural and Horticultural Crops, vol. 4, 3rd edn. pp. 2250–2269. Springer-Verlag, Vienna.
Inada, I. & M. Endo, 1999. Cytogenetic relationship between Allium fistulosum L. and A. schoenoprasum L. J Japan Soc Hort Sci 68: 960–966.
Inden, H. & T. Asahira, 1990. Japanese bunching onion (Allium fistulosum L.). In: J.L. Brewster & H.D. Rabinowitch (Eds.), Onions and Allied Crops, vol. 3, Biochemistry, Food Science, and Minor Crops. pp. 159–178. CRC Press, Boca Raton, Florida.
Keller, E.R.J., L. Schubert, J. Fuchs & A. Meister, 1996. Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization. Theor Appl Genet 92: 417–424.
Łoś-Kuczera, M., 1990. Composition and nutritive value. Food Products, 167.
Nomura, Y. & K. Makara, 1993. Production of interspecific hybrids between Rakkyo (Allium chinense) and some other Allium species by embryo rescue. Japan J Breed 43: 13–21.
Nomura, Y. & K. Makara, 1996. Morphological and agronomical characteristics in interspecific hybrid plants between Rakkyo (Allium chinense) and other edible Allium species. Breed Sci 46: 17–22.
Peffley, E.B. & A. Hou, 2000. Bulb-type onion introgressants possessing Allium fistulosum L. genes recovered from interspecific hybrid backcrosses between A. cepa L. and A. fistulosum L. Theor Appl Genet 100: 528–534.
Poulsen, N., 1990. Chives, Allium schoenoprasum L. In: J.L. Brewster & H.D. Rabinowitch (Eds.), Onions and Allied Crops, vol. 3 Biochemistry, Food Science, and Minor Crops. pp. 231–250. CRC Press, Boca Raton, Florida.
Steinmetz, K.A., 1996. Vegetables, fruit, and cancer prevention: A review. J Am Diet Assoc 96: 1027–1039.
Takagi, H., 1987. Dormancy and seasonal changes of growth activities in Asatsuki (Allium schoenoprasum L. var. schoenoprasum). J Japan Soc Hort Sci 56: 60–69 (In Japanese).
White, T.J., T. Bruns, S. Lee & J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M. Innes, D. Gelfand, J. Sninsky & T.J. White (Eds.), A guide to methods and applications, pp. 315–322. Academic Press Inc., San Diego.
Yamaura, A., 1955. Chromosome numbers of some plants raised from interspecific crossing and of their offspring in Allium. La Kromosomo 25–26: 894–898 (In Japanese).
Yanagino, T., E. Sugawara, M. Watanabe & Y. Takahata, 2003. Production and characterization of an interspecific hybrid between leek and garlic. Theor Appl Genet 107: 1–5.
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Umehara, M., Sueyoshi, T., Shimomura, K. et al. Interspecific hybrids betweenAllium fistulosumandAllium schoenoprasumreveal carotene-rich phenotype. Euphytica 148, 295 (2006). https://doi.org/10.1007/s10681-005-9029-8
- Allium fistulosumL.
- Allium schoenoprasumL.
- interspecific hybrid
- ovary culture