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Detection of linkage disequilibrium QTLs controlling low-temperature growth and metabolite accumulations in an admixed breeding population of Leymus wildryes

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Abstract

Low-temperature soluble carbohydrate accumulations are commonly associated with anthocyanin coloration, attenuated growth, and cold adaptation of cool-season grasses. A total of 647 AFLP markers were tested for associations with anthocyanin coloration, tiller formation, leaf formation, cumulative leaf length, percent soluble carbohydrate, and dry matter regrowth among replicated clones of an admixed Leymus wildrye breeding population evaluated in low-temperature growth chambers. The admixed breeding population was derived from a heterogeneous population of L. cinereus × L. triticoides F1 hybrids, with two additional generations of open pollination. Two AFLP linkage maps, constructed from two full-sib mapping populations derived from the same F1 hybrid population, were integrated to produce a framework consensus map used to examine the distribution of marker-trait associations in the admixed F1OP2 population. Thirty-seven linkage blocks, spanning 258 cM (13.6%) of the 1895 cM consensus map, contained 119 (50%) of the 237 markers showing at least one possible trait association (P < 0.05). Moreover, 28 (68%) of the 41 most significant marker-trait associations (P < 0.005) were located in 15 QTL linkage blocks spanning 112.9 cM (6%) of the linkage map. The coincidence of these 28 significant marker-trait associations, and many less significant associations, in 15 relatively small linkage blocks (0.6 cM to 21.3 cM) provides evidence of admixture linkage disequilibrium QTLs (ALD QTLs) in this heterogeneous breeding population. At least four of the remaining 13 putative marker-trait associations (P < 0.005) were located in genetic map regions lacking other informative markers. The complexity of marker-trait associations results from heterogeneity within and substantial divergence among the parental accessions.

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Author notes

  1. Z.-M. Hu

    Present address: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, P.R. China

  2. X.-L. Wu

    Present address: University of Missouri-Columbia, Columbia, Missouri, 65211, U.S.A.

Authors and Affiliations

  1. U.S. Department of Agriculture, Agriculture Research Service, Forage and Range Research Laboratory, Utah State University, Logan, Utah, 84322-6300, U.S.A.

    Z.-M. Hu, X.-L. Wu, S. R. Larson, R. R.-C. Wang, T. A. Jones & N. J. Chatterton

  2. U.S. Army Corps of Engineers, Engineering Research and Development Center, Cold Regions Research and Engineering Laboratory, 72 Lyme Road, Hanover, New Hampshire, 03755-1290, U.S.A.

    A. J. Palazzo

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  1. Z.-M. Hu
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  2. X.-L. Wu
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  3. S. R. Larson
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  4. R. R.-C. Wang
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  5. T. A. Jones
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  6. N. J. Chatterton
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  7. A. J. Palazzo
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Correspondence to S. R. Larson.

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Hu, ZM., Wu, XL., Larson, S.R. et al. Detection of linkage disequilibrium QTLs controlling low-temperature growth and metabolite accumulations in an admixed breeding population of Leymus wildryes. Euphytica 141, 263–280 (2005). https://doi.org/10.1007/s10681-005-7791-2

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  • DOI: https://doi.org/10.1007/s10681-005-7791-2

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