Abstract
Allelism tests and molecular marker analyses were combined to characterize the genes that, proceeding from the germplasm lines ‘A493’ and ‘A321’, confer resistance to bean anthracnose in the new breeding lines ‘A1220’ and ‘A1231’, respectively, developed through backcross breeding, using the dry bean landrace ‘Andecha’ as the recurrent parent. Allelism tests indicate that resistance to race 38 of anthracnose in genotypes ‘A1220’, ‘A1231’, and ‘BAT 93’ and in the differential cultivars ‘PI 207262’ and ‘Mexico 222’ is determined by different dominant alleles at the same locus. Therefore, the results obtained suggest that the so far considered as different genes Co-3 (described as present in ‘Mexico 222’) and Co-9 (described as present in ‘BAT 93’) are alleles of the same gene. RAPD markers OB12350, OAH181100, and OY171100 and SCAR markers SI19 and SW12 were found to be linked to the resistance gene. Data indicate that the resistance genes to race 38 present in these materials are alleles of the same R gene cluster located in linkage group B4, because markers OY171100, SI19 and SW12 were previously linked to this cluster. The SCAR markers SB12 and SAH18 were developed from RAPDs OB12350 and OAH181100, respectively, and a genetic map including the resistance gene and markers SB12, OY171100, SAH18, SW12 and SI19 was made using a F2 segregating population of 72 individuals derived from the cross ‘Andecha’ × ‘A493’.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alzate-Marin, A.L., G.S. Baía, T.J. de Paula Jr, G. Assis de Carvalho, E. Gonçalves de Barros & M.A. Moreira, 1997. Inheritance of anthracnose resistance in common bean differential cultivar AB136. Plant Dis 81: 996–998.
Alzate-Marin, A.L., K. Souza de Almeida, E. Gonçalves de Barros & M. Alves Moreira, 2001. Preliminary results of allelism studies for anthracnose resistance genes of common bean cultivar PI 207262. Annu Rep Bean Improv Coop 44: 113–114.
Alzate-Marin, A.L., M.R. Costa, K.M. Arruda, E. Gonçalves de Barros & M. Alves Moreira, 2003. Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar. Euphytica 133: 165–169.
Balardin, R.S., A.M. Jarosz & J.D. Kelly, 1997. Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central and North America. Phytopathology 87: 1184–1191.
Bannerot, H., M. Deieux & G. Fouilloux, 1971. Mise en evidence d’un second gene de resistance totale a l’anthracnose chez le haricot. Ann Amélior Plantes 21: 83–85.
Burkholder, W.H., 1918. The production of an anthracnose-resistant white marrow bean. Phytopathology 8: 353–359.
Dixon, M., K. Hatzixanthis, D. Jones, K. Harrison & J. Jones, 1998. The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number. Plant Cell 10: 1915–1925.
Ferreira, J.J., M.A. Fueyo, A.J. González & R. Giraldez, 1998. Pathogenic variability within Colletotrichum lindemuthianum in Northern Spain. Annu Rep Bean Improv Coop 41: 163–164.
Fouilloux, G., 1976. L’anthracnose du haricot (Colletotrichum lindemuthianum, Sacc et Magn): Nouvelles sources de résistance et nouvelles races physiologiques. Ann Amélior Plantes 26: 443–453.
Freyre, R., P.W. Skorch, V. Geffroy, A.-F. Adam-Blondon, A. Shirmohamadali, W.C. Johnson, V. Llaca, R.O. Nodari, P.A. Pereira, S.M. Tsai, J. Tohme, M. Dron, J. Nienhuis, C.E. Vallejos & P. Gepts, 1998. Towards an integrated linkage map of common bean. Part 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97: 847–856.
Geffroy, V., D. Sicard, J.C.F. de Oliveira, M. Sévignac, S. Cohen, P. Gepts, C. Neema, T. Langin & M. Dron, 1999. Identification of an ancestral resistance gene cluster involved in the coevolution process between Phaseolus vulgaris and its fungal pathogen Colletotrichum lindemuthianum. Mol Plant-Microbe Interact 12: 774–784.
Graham, M.A., L.F. Marek & R.C. Shoemaker, 2002. Organization, expression and evolution of a disease resistance gene cluster in soybean. Genetics 162: 1961–1977.
Haley, S.D., L.K. Afanador & J.D. Kelly, 1994. Identification and application of a random amplified polymorphic DNA marker for the I gene (Potyvirus resistance) in common bean. Phytopathology 84: 157–160.
Kelly, J.D. & R.A. Young, 1996. Proposed symbols for anthracnose resistance genes. Annu Rep Bean Improv Coop 39: 20–24.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincon & L. Newburgh, 1987. MAPMAKER: An interactive computing package for constructing primary genetic linkage maps of experimental and natural population. Genomics 1: 174–181.
Mastenbroek, C., 1960. A breeding programme for resistance to anthracnose in dry shell haricot beans based on a new gene. Euphytica 9: 177–184.
Melotto, M. & J.D. Kelly, 1998. SCAR markers linked to major disease resistance genes in common bean. Annu Rep Bean Improv Coop 41: 64–65.
Melotto, M. & J.D. Kelly, 2000. An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica 116: 143–149.
Méndez de Vigo, B., C. Rodríguez, A. Pañeda, R. Giraldez & J.J. Ferreira, 2002. Development of a SCAR marker linked to Co-9 in common bean. Annu Rep Bean Improv Coop 45: 116–117.
Menezes, J.R. & J.C. Dianese, 1988. Race characterization of Brazilian isolates of Colletotrichum lindemuthianum and detection of resistance to anthracnose in Phaseolus vulgaris L. Phytopathology 78: 650–655.
Miklas, P.N., R. Delorme, V. Stone, M.J. Daly, J.R. Stavely, J.R. Steadman, M.J. Bassett & J.S. Beaver, 2000. Bacterial, fungal, virus disease loci mapped in a recombinant inbred common bean population (“Dorado/XAN 176”). J Am Soc Hortic Sci 125: 476–481.
Miklas, P.N., M.A. Pastor-Corrales, G. Jung, D.P. Coyne, J.D. Kelly, P.E. McClean & P. Gepts, 2002. Comprehensive linkage map of bean rust resistance genes. Annu Rep Bean Improv Coop 45: 125–129.
Pastor-Corrales, M.A., 1991. Estandarización de variedades diferenciales y de designación de razas de Colletotrichum lindemuthianum (Abstr). Phytopathology 81: 694.
Pastor-Corrales, M.A. & J.C. Tu, 1989. Anthracnose. In: H.F. Schwartz & M.A. Pastor-Corrales (Eds.), Bean Production Problems in the Tropics, pp. 77–104. CIAT, Colombia.
Young, R.A. & J.D. Kelly, 1996. Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. Plant Dis 80: 650–654.
Young, R.A. & J.D. Kelly, 1997. RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Sci 37: 940–946.
Young, R.A., M. Melotto, R.O. Nodari & J.D. Kelly, 1998. Marker-assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar, ‘G2333’. Theor Appl Genet 96: 87–94.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Méndez-Vigo, B., Rodríguez-Suárez, C., Pañeda, A. et al. Molecular markers and allelic relationships of anthracnose resistance gene cluster B4 in common bean. Euphytica 141, 237–245 (2005). https://doi.org/10.1007/s10681-005-7075-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-7075-x