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Marker assisted polycross breeding to increase diversity and yield in perennial ryegrass (Lolium perenne L.)

Euphytica volume 146, pages 55–65 (2005)Cite this article

Summary

In this study, the application of molecular markers to optimise genetic diversity in a polycross breeding program of perennial ryegrass (Lolium perenne L.) was evaluated. The genetic diversity among 98 potential parental plants from three maturity groups (early, intermediate and late flowering) was investigated using amplified fragment length polymorphism (AFLP) markers. For each maturity group, two polycrosses of six parental plants with contrasting levels of genetic diversity were composed. Average genetic diversity among parents selected for narrow polycrosses was 36% lower than among parents selected for wide polycrosses. Diversity within first generation synthetic progenies (Syn1) was proportional to the diversity within the respective parental polycrosses. However, differences were less pronounced with Syn1 progenies from narrow polycrosses showing 16% reduced diversity when compared to Syn1 progenies from wide polycrosses. Multivariate analyses allowed for a clear separation of the six Syn1 progenies based on AFLP markers and demonstrated their genetic distinctness. Evaluation of dry matter yield, date of ear emergence and stem length of Syn1 and Syn2 progenies showed progenies from wide polycrosses to be constantly higher yielding when compared to progenies from narrow polycrosses. On the other hand, there was no significant difference in variability for the two morphological traits between progenies of narrow- and wide polycrosses. The results presented here provide evidence for an efficient application of molecular markers to select genetically diverse polycross parents which resulted in an average yield increase of 3.8%.

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References

  • Armstead, I.P., L.B. Turner, M. Farrell, L. Skot, P. Gomez, T. Montoya, I.S. Donnison, I.P. King & M.O. Humphreys, 2004. Synteny between a major heading-date QTL in perennial ryegrass (Lolium Perenne L.) and the HD3 heading-date locus in rice. Theor Appl Genet 108: 822–828.

    Article  PubMed  CAS  Google Scholar 

  • Barcaccia, G., E. Albertini, S. Tavoletti, M. Falcinelli & F. Veronesi, 1999. AFLP fingerprinting in Medicago spp.: Its development and application in linkage mapping. Plant Breed 118: 335–340.

    Article  CAS  Google Scholar 

  • Barcaccia, G., R. Torricelli, L. Russi, B. Lucaroni, F. Bozzo, F. Veronesi & M. Falcinelli, 1998. Morphological and molecular characterization of two cultivars of Lolium perenne L.: An example of calculating the genetic distance between a registered and a newly-constituted cultivar. J Appl Seed Prod 16: 39–45.

    Google Scholar 

  • Bert, P.F., G. Charmet, P. Sourdille, M.D. Hayward & F. Balfourier, 1999. A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers. Theor Appl Genet 99: 445–452.

    Article  CAS  Google Scholar 

  • Brummer, C.E., 1999. Capturing heterosis in forage crop cultivar development. Crop Sci 39: 943–954.

    Google Scholar 

  • Burkhammer, R.L., S.P. Lanning, R.J. Martens, J.M. Martin & L.E. Talbert, 1998. Predicting progeny variance from parental divergence in hard red spring wheat. Crop Sci 38: 243–248.

    Article  Google Scholar 

  • Cerna, F.J., S.R. Cianzio, A. Rafalski, S. Tingey & D. Dyer, 1997. Relationship between seed yield heterosis and molecular marker heterozygosity in soybean. Theor Appl Genet 95: 460–467.

    Article  CAS  Google Scholar 

  • de Araujo, M.R.A., B. Coulman, D. Somers & Y. Ferdinandez, 2003. Random amplified polymorphic DNA variation within and among meadow bromegrass progeny types. Plant Breed 122: 416-420.

    Article  CAS  Google Scholar 

  • Duvick, D.N., 1992. Genetic contributions to advances in yield of U.S. maize. Maydica 37: 69–79.

    Google Scholar 

  • EC, 2004. Agriculture in the European Union – Statistical and economic information 2003. European Union, Directorate-General for Agriculture, Brussels, Belgium.

  • Excoffier, L., P.E. Smouse & J.M. Quattro, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479–491.

    PubMed  CAS  Google Scholar 

  • Fernando, W.M.U., M.D. Hayward & M.J. Kearsey, 1997. Isozyme and quantitative traits polymorphisms in European provenances of perennial ryegrass (Lolium perenne L.). Euphytica 93: 263–269.

    CAS  Google Scholar 

  • Gilliland, T.J., R. Coll, E. Calsyn, M. De Loose, M.J.T. van Eijk & I. Roldán-Ruiz, 2000. Estimating genetic conformity between related ryegrass (Lolium) varieties. II. Morphology and biochemical characterisation. Mol Breed 6: 569–580.

    Article  Google Scholar 

  • Golembiewski, R.C., T.K. Danneberger & P.M. Sweeney, 1997. Potential of RAPD markers for use in the identification of creeping bentgrass cultivars. Crop Sci 37: 212–214.

    CAS  Article  Google Scholar 

  • Guthridge, K.M., M.P. Dupal, R. Kölliker, E.S. Jones & J.W. Forster, 2001. AFLP analysis of genetic diversity within and between populations of perennial ryegrass (Lolium perenne L.). Euphytica 122: 191–201.

    Article  CAS  Google Scholar 

  • Huff, D.R., 1997. RAPD characterisation of heterogeneous perennial ryegrass cultivars. Crop Sci 37: 557–564.

    CAS  Article  Google Scholar 

  • Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223–270.

    Google Scholar 

  • Jones, E.S., M.P. Dupal, J.L. Dumsday, L.J. Hughes & J.W. Forster, 2002. An SSR-based genetic linkage map for perennial ryegrass (Lolium Perenne L.). Theor Appl Genet 105: 577–584.

    PubMed  CAS  Google Scholar 

  • Joyce, T.A., M.T. Abberton, T.P.T. Michaelson-Yeates & J.W. Forster, 1999. Relationships between genetic distance measured by RAPD-PCR and heterosis in inbred lines of white clover (Trifolium repens L.). Euphytica 107: 159–165.

    Article  Google Scholar 

  • Kölliker, R., D. Herrmann, B. Boller & F. Widmer, 2003. Swiss Mattenklee landraces, a distinct and diverse genetic resource of red clover (Trifolium pratense L.). Theor Appl Genet 107: 306–315.

    PubMed  Google Scholar 

  • Kölliker, R., F.J. Stadelmann, B. Reidy & J. Nösberger, 1999. Genetic variability of forage grass cultivars: A comparison of Festuca pratensis Huds., Lolium perenne L. and Dactylis glomerata L. Euphytica 106: 261–270.

    Google Scholar 

  • Liu, X.C., K. Ishiki & W.X. Wang, 2002. Identification of AFLP markers favorable to heterosis in hybrid rice. Breed Sci 52: 201–206.

    Article  CAS  Google Scholar 

  • Lynch, M. & B. Walsh, 1998. Genetic analysis of quantitative traits. Sinauer Associates Inc., Sunderland, Massachusetts, USA.

  • Melchinger, A.E., 1999. Genetic diversity and heterosis. In: J.G. Coors & S. Pandey (Eds.), Genetic and Exploitation of Heterosis in Crops, pp. 99–118. ASA-CSA-SSSA, Madison, Wisconsin, USA.

  • Reed, D.H. & R. Frankham, 2003. Correlation between fitness and genetic diversity. Conserv Biol 17: 230–237.

    Article  Google Scholar 

  • Reif, J.C., A.E. Melchinger, X.C. Xia, M.L. Warburton, D.A. Hoisington, S.K. Vasal, G. Srinivasan, M. Bohn & M. Frisch, 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize populations. Crop Sci 43: 1275–1282.

    Article  Google Scholar 

  • Rohlf, F.J., 2000. NTSYS-pc: Numerical taxonomy and multivariate analysis system, version 2.1. Exter Publishers, Setauket, NY.

    Google Scholar 

  • Roldán-Ruiz, I., E. Calsyn, T.J. Gilliland, R. Coll, M.J.T. van Eijk & M. De Loose, 2000a. Estimating genetic conformity between related ryegrass (Lolium) varieties. II. AFLP characterization. Mol Breed 6: 593–602.

    Google Scholar 

  • Roldán-Ruiz, I., J. Dendauw, E. VanBockstaele, A. Depicker & M. De Loose, 2000b. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6: 125–134.

    Google Scholar 

  • Roldán-Ruiz, I., F.A. Van Eeuwijk, T.J. Gilliland, P. Dubreuil, C. Dillmann, J. Lallemand, M. De Loose & C.P. Baril, 2001. A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties. Theor Appl Genet 103: 1138–1150.

    Google Scholar 

  • Rumbaugh, M.D., J.L. Caddel & D.E. Rowe, 1988. Breeding and quatitative genetics. In: A.A. Hanson, D.K. Barnes, et al. (Eds.), Alfalfa and Alfalfa Improvement. Agronomy Monograph 29, pp. 777–808. ASA, CSSA, SSA, Madison, Wisconsin, USA.

  • Sant, V.J., A.G. Patankar, N.D. Sarode, L.B. Mhase, M.N. Sainani, R.B. Deshmukh, P.K. Ranjekar & V.S. Gupta, 1999. Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars. Theor Appl Genet 98: 1217–1225.

    Article  CAS  Google Scholar 

  • Sawbridge, T., E.K. Ong, C. Binnion, M. Emmerling, R. Mcinnes, K. Meath, N. Nguyen, K. Nunan, M. O'neill, F. O'toole, C. Rhodes, J. Simmonds, P. Tian, K. Wearne, T. Webster, A. Winkworth & G. Spangenberg, 2003. Generation and analysis of expressed sequence tags in perennial ryegrass (Lolium perenne L.). Plant Sci. 165: 1089–1100.

    CAS  Google Scholar 

  • Silvertown, J.W. & J. Lovett Doust, 1993. Introduction to Plant Population Biology. Blackwell Scientific Publications, Oxford.

    Google Scholar 

  • Skot, L., N.R. Sackville Hamilton, S. Mizen, K.H. Chorlton & I.D. Thomas, 2002. Molecular genecology of temperature response in Lolium perenne. 2. Association of AFLP markers with ecogeography. Mol Ecol 11: 1865–1876.

    Article  PubMed  CAS  Google Scholar 

  • Souza, E. & M.E. Sorrells, 1991. Prediction of progeny variation in oat from parental genetic relationships. Theor Appl Genet 82: 233–241.

    Article  Google Scholar 

  • ter Braak, C.J.F. & P. Smilauer, 2002. CANOCO reference manual and CanoDraw for Windows user's guide: Software for canonical community ordination (version 4.5). Microcomputer power, Ithaca, New York, USA.

    Google Scholar 

  • Ubi, B.E., R. Kölliker, M. Fujimori & T. Komatsu, 2003. Genetic diversity in diploid cultivars of rhodesgrass determined on the basis of amplified fragment length polymorphism markers. Crop Sci 43: 1516–1522.

    Article  Google Scholar 

  • UPOV, 1990. Guidelines for the conduct of tests for distinctness, homogeneity and stability. International Union for the Protection of New Varieties of Plants, Geneva, Switzerland.

  • UPOV, 2002. General introduction to the examination of distinctness, uniformity and stability and the development of harmonized descriptions of new varieties of plants. International Union for the Protection of New Varieties of Plants, Geneva, Switzerland.

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Vandelee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP – A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.

    PubMed  CAS  Google Scholar 

  • Wilkins, P.W. & M.O. Humphreys, 2003. Progress in breeding perennial forage grasses for temperate agriculture. J Agric Sci 140: 129–150.

    Article  CAS  Google Scholar 

  • Xu, W.J., S.S. Virmani, J.E. Hernandez, L.S. Sebastian, E.D. Redona & Z.K. Li, 2002. Genetic diversity in the parental lines and heterosis of the tropical rice hybrids. Euphytica 127: 139–148.

    Article  CAS  Google Scholar 

  • Yamada, T., E.S. Jones, N.O.I. Cogan, A.C. Vecchies, T. Nomura, H. Hisano, Y. Shimamoto, K.F. Smith, M.D. Hayward & J.W. Forster, 2004. QTL Analysis of morphological, developmental, and winter hardiness-associated traits in perennial ryegrass. Crop Sci 44: 925–935.

    CAS  Article  Google Scholar 

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Authors and Affiliations

  1. Agroscope FAL Reckenholz, Swiss Federal Research Station for Agroecology and Agriculture, P.O. Box, 8046, Zurich, Switzerland

    Roland KöLliker, Beat Boller & Franco Widmer

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  1. Roland KöLliker
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  2. Beat Boller
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  3. Franco Widmer
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Correspondence to Roland KöLliker.

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KöLliker, R., Boller, B. & Widmer, F. Marker assisted polycross breeding to increase diversity and yield in perennial ryegrass (Lolium perenne L.). Euphytica 146, 55–65 (2005). https://doi.org/10.1007/s10681-005-6036-8

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  • DOI: https://doi.org/10.1007/s10681-005-6036-8

Key words

  • AFLP
  • genetic diversity
  • heterosis
  • polycross
  • uniformity
  • yield