Skip to main content

Molecular characterization of genetic diversity in European germplasm of perennial ryegrass

Summary

Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate grassland agriculture. The level and distribution of genetic variation in gene bank ecotype collections is still largely unknown but of great interest for the planning of breeding programs. The objectives of this study were to (i) assess the molecular diversity of Polish ecotypes of perennial ryegrass, and (ii) compare the relationship between this group and German ecotypes and European cultivars investigated previously. A total number of 166 polymorphic marker bands were detected among the 171 individual plants of the 9 Polish ecotypes. In a joint analysis with 9 Polish and 22 German ecotypes, and 22 European cultivars 172 polymorphic RAPD markers could be found. Genetic distance among the Polish ecotypes ranged from 0.31 to 0.51, while for all 53 populations a broader range was detected (0.25–0.67). An analysis of molecular variance (AMOVA) revealed a much larger variation within populations (71%) than among them (29%). The Polish ecotypes contained the highest within population variation (74%). The largest among group difference (15%) was found between the Polish ecotypes versus all other accessions. We conclude that the Polish ecotypes represent a valuable genetic resource for enlarging the genetic variation in the West European germplasm pool of perennial ryegrass.

This is a preview of subscription content, access via your institution.

References

  • Bolaric, S., S. Barth, A.E. Melchinger & U.K. Posselt, 2005a. Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breeding (in press).

  • Bolaric, S., S. Barth, A.E. Melchinger & U.K. Posselt, 2005b. Molecular genetic diversity within and among German ecotypes in comparison to European perennial ryegrass cultivars. Plant Breeding (in press).

  • Brown, A.H.D., 1989. Core collections: A practical approach to genetic resources management. Genome 31: 818–824.

    Google Scholar 

  • Bundessortenamt, 2002. Beschreibende Sortenliste. Landbuchverlag, Hannover.

  • Cresswell, A., N.R. Sackville Hamilton, A.K. Roy & M.F. Viegas, 2001. Use of amplified fragment length polymorphism markers to assess genetic diversity of Lolium species from Portugal. Mol Ecol 10: 229–241.

    Article  PubMed  CAS  Google Scholar 

  • Fernando, W.M.U., M.D. Hayward & M.J. Kearsey, 1997. Isozyme and quantitative traits polymorphisms in European provenances of perennial ryegrass (Lolium perenne L.). Euphytica 93: 263–269.

    CAS  Google Scholar 

  • Ghesquiere, A., E. Calsyn, J. Baert & J. De Riek, 2003. Genetic diversity between and within ryegrass populations of the ECP/GR collection by means of AFLP markers. Czech J Genet Plant Breed 39: 333–336.

    Google Scholar 

  • Guy, P., M. Ghesquiere, G. Charmet & J.-M. Prosperi, 1989. Pooling accessions: Advantage and disadvantages. Appendix V, pp. 35–49. Report of a working group on forages, Montpellier, France, 9–12 Jan. 1989. Int. Board Pl. Gen. Res., Rome, Italy.

  • Huff, D.R., 1997. RAPD characterization of heterogenous perennial ryegrass cultivars. Crop Sci 17: 557–564.

    Google Scholar 

  • Humphreys, M.O., 2003. Utilization of plant genetic resources in breeding for sustainability. Plant Genet Resour 1: 11–18.

    Article  Google Scholar 

  • Kölliker, R., F.J. Stadelmann, B. Reidy & J. Noesberger, 1999. Genetic variability of forage grass cultivars: A comparison of Festuca pratensis Huds., Lolium perenne L., and Dactylis glomerata L. Euphytica 106: 261–270.

    Google Scholar 

  • Melchinger, A.E., 1999. Genetic diversity and heterosis. Chapter 10. In: J.G. Coors and S. Pandey (Eds.), The genetics and exploitation of heterosis in crops. CSSA, Madison, WI., pp. 99–118.

    Google Scholar 

  • Paul, C., 1989. Pre-breeding in genetic resources of perennial ryegrass (Lolium perenne L.). Report of a working group on forages, Appendix V, pp. 76–88. Montpellier, France, 9–12 Jan. 1989. International Board on Plant Genetic Research, Rome, Italy.

  • Paul, Chr., U.K. Posselt & H. Scheller, 1994. Pre-breeding in genetic resources of perennial ryegrass (Lolium perenne L.) (in German). Vortr. Pflanzenzüchtg. 27: 200–204.

    Google Scholar 

  • Posselt, U.K., 2000. Genetische Diversität bei Wildformen und Zuchtsorten von Lolium perenne L. Schriftenr. Vegetationskunde 32: 79–85.

    Google Scholar 

  • Roldan-Ruiz, I., F.A. van Eeuwijk, T.J. Gilliland, P. Dubreuil, C. Dillmann, J. Lallemand, M. De Loose & C.P. Baril, 2001. A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties. Theor Appl Genet 103: 1138–1150.

    CAS  Google Scholar 

  • Rohlf, F.J., 1998. NTSYS-pc ver. 2.0. Numerical taxonomy and multivariate analysis system. Exeter Publ., Setauket, NY.

    Google Scholar 

  • SAS Institute, 1990. SAS/STAT User's Guide. Release 6.03 Ed. SAS Institute Inc., Cary, NC.

    Google Scholar 

  • Schneider, S., J.M. Kueffer, D. Roessli & L. Excoffier, 1997. Arlequin ver. 1.1: A software for population genetic data analysis. Genetic and biometry laboratory, University of Geneva, Switzerland.

    Google Scholar 

  • Skøt, L., N.R. Sackville Hamilton, S. Mizen, K.H. Chorlton & I.D. Thomas, 2002. Molecular genecology of temperature response in Lolium perenne: 2. Association of AFLP markers with ecogeography. Mol Ecol 11: 1865–1876.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to U. K. Posselt.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bolaric, S., Barth, S., Melchinger, A.E. et al. Molecular characterization of genetic diversity in European germplasm of perennial ryegrass. Euphytica 146, 39–44 (2005). https://doi.org/10.1007/s10681-005-4439-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-005-4439-1

Key words

  • AMOVA
  • diversity
  • ecotypes
  • genetic distance
  • Lolium perenne L.
  • RAPD marker