Abstract
Broccoli (Brassica oleracea L., Italica Group) is a source of glucosinolates and their respective isothiocyanate metabolites that are believed to have chemoprotective properties in humans. Glucoraphanin (4-methylsulfinyl-butyl glucosinolate) is a predominant glucosinolate of broccoli. Its cognate isothiocyanate, sulforaphane, has proven a potent inducer of phase II detoxification enzymes that protect cells against carcinogens and toxic electrophiles. Little is known about the genetic combining ability for glucosinolate levels or the types of genetic variation (i.e., additive vs. dominance) that influence those levels in broccoli. In this study, a diallel mating design was employed in two field experiments to estimate combining abilities for glucoraphanin content. The diallel population was developed by crossing nine doubled-haploid (inbred) parents in all possible combinations (36), excluding the reciprocals. Horticultural traits of all entries were assessed on a plot basis. In fall 2001, glucoraphanin concentration of broccoli heads ranged from 0.83 to 6.00 μmol/gdw, and in spring 2002, ranged from 0.26 to 7.82 μmol/gdw. In both years, significant general combining ability was observed for glucoraphanin concentration and total head content, days from transplant to harvest, head weight, and stem diameter. Conversely, no significant specific combining ability was observed for any trait in either year. Results indicate that a given inbred will combine with others to make hybrids with relatively predictable levels of head glucoraphanin as well as, other important horticultural traits. This should allow identification of inbreds that typically contribute high glucoraphanin levels when hybridized with others.
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Abbreviations
- GCA:
-
general combining ability
- SCA:
-
specific combining ability
References
Baker, R.J., 1978. Issues in diallel analysis. Crop Sci 18(4): 533–536.
Brown, A.F., G. Yousef, E. Jeffrey, B. Klein, M. Wallig, M. Kushad & J. Juvik, 2002. Glucosinolate profiles in broccoli: Variation in levels and implication in breeding for cancer chemoprotection. J Am Soc Hort Sci 127: 807–813.
Fahey, J.W., Y. Zhang & P. Talalay, 1997. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA 94: 10367–10372.
Falconer, D.S., 1981. Introduction to Quantitative Genetics. Second Edition, Longman Group Ltd., London.
Farnham, M.W., K. Stephenson & J.W. Fahey, 2000. Capacity of broccoli to induce a mammalian chemoprotective enzyme varies among inbred lines. J Am Soc Hort Sci 125(4): 482–488.
Farnham, M.W., P. Wilson, K. Stephenson & J.W. Fahey, 2004. Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli. Plant Breed 123: 60–65.
Faulkner, K., R. Mithen & G. Williamson, 1998. Selective increase of the potential anticarcinogen 4-methylsulphinylbutyl glucosinolate in broccoli. Carcinogenesis 19(4): 605–609.
Fehr, W.R., 1987. Principles of Cultivar Development: Theory and Technique. Macmillan Publishing Co., New York.
Giamoustaris, A. & R. Mithen, 1996. Genetics of aliphatic glucosinolates. IV. Side-chain modification in Brassica oleracea. Theor Appl Genet 93: 1006–1010.
Graham, S., H. Dayal, M. Swanson, A. Mittelman & G. Wilkinson, 1978. Diet in the epidemiology of cancer of the colon and rectum. J Natl Cancer Inst 61: 709–714.
Griffing, B., 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Aust J Biol Sci 9: 463–493.
Hansen, M., P. Moller, H. Sorensen & M. Cantwell de Trejo, 1995. Glucosinolates in broccoli stored under controlled atmosphere. J Am Soc Hort Sci 120: 1069–1074.
Hecht, S., 2000. Inhibition of carcinogenesis by isothiocyanates. Drug Metab Rev 32(3/4): 395–411.
Hulbert, S. & T.J. Orton, 1984. Genetic and environmental effects on mean maturity date and uniformity in broccoli. J Am Soc Hort Sci 109: 487–490.
Jain, M., G. Hislop, G. Howe & P. Ghardirian, 1999. Plant foods, antioxidants, and prostate cancer risk: Findings from case-control studies in Canada. Nutr Cancer 34: 173–184.
Kohlmeir, L. & L. Su, 1997. Cruciferous vegetable consumption and colorectal cancer risk: Meta-analysis of the epidemiological evidence. FASEB J 11: 2141.
Kolonel, L., J.H. Hankin, A. Wu, R. Gallagher, L. Wilkens, E. John, G. Howe, D. Dreon, D. West & R. Paffenberger, Jr., 2000. Vegetables, fruits, legumes and prostate cancer: A multicenter case-control study. Cancer Epidemiol Biomark Prev 9: 795–804.
Kushad, M., A. Brown, A. Kurlich, J. Juvik, B. Klein, M. Wallig & E. Jeffrey, 1999. Variation of glucosinolates in vegetable crops of Brassica oleracea. J Agric Food Chem 47: 1541–1548.
Li, G., A. Riaz, S. Goyal, S. Abel & C. Quiros, 2001. Inheritance of three major genes involved in the synthesis of aliphatic glucosinolates in Brassica oleracea. J Am Hort Sci 126(4): 427–431.
London, S., J. Yuan, F. Chung, Y. Gao, G. Coetzee, R. Ross & M. Yu, 2000. Isothiocyanates, glutathione S-transferase M1 and T1 polymorphisms, and lung cancer risk: A prospective study of men in Shanghai, China. Lancet 356: 724–729.
Michaud, D.S., D. Speigelman, S. Clinton, E. Rimm, W. Willett & E. Giavannucci, 1999. Fruit and vegetable intake and incidence of bladder cancer in a male prospective cohort. J Natl Cancer Inst 91: 605–613.
Mithen, R., K. Faulkner, R. Magrath, P. Rose, G. Williamson & J. Marquez, 2003. Development of isothiocyanate-enriched broccoli, and its enhanced ability to induce phase 2 detoxification enzymes in mammalian cells. Theor Appl Genet 106: 727–734.
Rosa, E. & A. Rodrigues, 2001. Total and individual glucosinolate content in 11 broccoli cultivars grown in early and late seasons. HortScience 81(3): 295–299.
Shapiro, T.A., J. Fahey, K. Wade, K. Stephenson & P. Talalay, 1998. Human metabolism and excretion of cancer chemoprotective glucosinolates and isothiocyanates of cruciferous vegetables. Cancer Epidemiol Biomark Prev 7: 1091–1100.
Terry, P., A. Wolk, I. Persson & C. Magnusson, 2001. Brassica vegetables and breast cancer risk. JAMA 286: 2975–2977.
Troyer, J., K. Stephenson & J. Fahey, 2001. Analysis of glucosinolates from broccoli and other cruciferous vegetables by hydrophilic interaction liquid chromatography. J Chromatogr A 919: 299–304.
Vallejo, F., F. Tomas-Barberan, A. Benavente-Garcia & C. Garcia-Viguera, 2003. Total and individual glucosinolate contents in inflorescences of eight broccoli cultivars grown under various climatic and fertilization conditions. J Sci Food Agric 83(4): 307–313.
Verhoeven, D., H. Verhagen, R. Goldbohm, P. van den Brandt & G. van Poppel, 1997. A review of the mechanisms underlying anticarcinogenicity by brassica vegetables. Chem Biol Interact 103: 79–129.
Zhang, Y., P. Talalay, C. Cho & G. Posner, 1992. A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure. Proc Natl Acad Sci USA 89: 2399–2403.
Zhang, Y., W. Kensler, C. Cho, G. Posner & P. Talalay, 1994. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothicyanates. Proc Natl Acad Sci USA 91: 3147–3150.
Zhang, S., D.J. Hunter, B. Rosner, E. Giovannucci, G. Colditz, F. Speizer & W. Willett, 2000. Intake of fruits, vegetables, and related nutrients and the risk of non-Hodgkin’s lymphoma among women. Cancer Epidemiol Biomark Prev 9: 477–485.
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Abercrombie, J.M., Farnham, M.W. & Rushing, J.W. Genetic combining ability of glucoraphanin level and other horticultural traits of broccoli. Euphytica 143, 145–151 (2005). https://doi.org/10.1007/s10681-005-3059-0
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DOI: https://doi.org/10.1007/s10681-005-3059-0