Abstract
Intergeneric hybrid plants between Colchicaceous ornamental plants, Sandersonia aurantiaca and Gloriosa rothschildiana, have successfully been produced via ovule culture. After 5 days of reciprocal cross-pollination, a few pollen tubes were observed in the ovary. Although seeds were obtained in both reciprocal cross-combinations, they did not germinate under ex vitro conditions. Ovules with placental tissues isolated 14 days after cross-pollination of S. aurantiaca × G. rothschildiana were cultured on a medium containing 0.01 mg l−1 each of α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), on which 41.5% of ovules swollen and produced callus-like structures within 10 weeks. When such swollen ovules were transferred to a medium containing 0.1 mg l−1 each of NAA and BA, 7.5% of the initially cultured ovules produced rhizome-like structures within 6 weeks. Among the rhizome-like structures, those derived from two independent ovules (3.7% of the initially cultured ovules) produced multiple shoots following transfer to a medium containing 0.25 mg l−1 NAA and 2.5 mg l−1 BA. Multiple shoot-derived plantlets were established on a plant growth regulator-free medium, and they were successfully transplanted to pots. Early verification of their hybridity was accomplished by flow cytometry (FCM) analysis, chromosome observation and rDNA analysis.
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References
Asano, Y., 1978. Studies on crosses between distantly related species of lilies. III. New hybrids through embryo culture. J Jpn Soc Hortic Sci 47: 401–414.
Custers, J.B.M. & J.H.W. Bergervoet, 1994. Micropropagation of Gloriosa: Towards a practical protocol. Sci Hortic 57:323–334.
Eason, J.R. & D. Webster, 1995. Development and senescence of Sandersonia aurantiaca Hook. flowers. Sci Hortic 63: 113–121.
Eason, J.R., E.R. Morgan, A.C. Mullan & G.K. Burge, 2001. Postharvest characteristics of Santonia ‘Golden Lights’ a new hybrid cut flower from Sandersonia aurantiaca × Littonia modesta. Postharvest Biol Technol 22: 93–97.
Griesbach, R.J., 1993. Creation of new flower colors in Ornithogalum via interspecific hybridization. J Amer Soc Hortic Sci 118: 409–414.
Ijiro, Y., H. Kitamura, K. Yamane, N. Fujishige & M. Yoshida, 2000. Seed emergence in sandersonia (Sandersonia aurantiaca Hook.). J Jpn Soc Hort Sci 69 (suppl 6): 306 (in Japanese).
{Kato, J., R. Ishikawa & M. Mii, 2001. Different genomic combinations in inter-section hybrids obtained from the crosses between Primula sieboldii (Section Cortusoides) and P. obconica (Section Obconicolisteri) by the embryo rescue technique. Theor Appl Genet 102: 1129–113.
Kho, Y.O. & J. Baër, 1968. Observing pollen tubes by means of fluorescence. Euphytica 17: 298–302.
Li, T.-H., Y. Niimi & M. Nakano, 1996. Pollen tube growth and seed set in stigmatic and cut-style pollinated Lilium longiflorum ‘Georgia’ flowers as influenced by pre-pollination. J Jpn Soc Hortic Sci 65: 135–144 (in Japanese with English summary).
Morgan, E.R., G.K. Burge, J.F. Seelye, M.E. Hopping, J.E. Grant, A.G.F. Warren & D. Brundell, 2001. Wide crosses in the Colchicaceae: Sandersonia aurantiaca (Hook.) × Littonia modesta (Hook.). Euphytica 121: 343–348.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Nakano, M. & M. Mii, 1993. Somatic hybridization between Dianthus chinensis and D. barbatus through protoplast fusion. Theor Appl Genet 86: 1–5.
Saito, H., K. Mizunashi, S. Tanaka, Y. Adachi & M. Nakano, 2003. Ploidy estimation in Hemerocallis species and cultivars by flow cytometry. Sci Hortic 97: 185–192.
Takaiwa, F., K. Oono, Y. Iida & M. Sugiura, 1985. The complete nucleotide sequence of a rice 25s rRNA gene. Gene 37: 255–259.
van Creij, M.G.M., D.M.F.J. Kerckhoffs & J.M. van Tuyl, 1997. Interspecific crosses in the genus Tulipa L.: Identification of pre-fertilization barriers. Sex Plant Reprod 10: 116–123.
van Tuyl, J.M., M.P. van Dien, M.G.M. van Creij, T.C.M. van Kleinwee, J. Franken & R.J. Bino, 1991. Application of in vitro pollination, ovary culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci 74: 115– 126.
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Nakamura, T., Kuwayama, S., Tanaka, S. et al. Production of intergeneric hybrid plants between Sandersonia aurantiaca and Gloriosa rothschildiana via ovule culture (Colchicaceae). Euphytica 142, 283–289 (2005). https://doi.org/10.1007/s10681-005-2416-3
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DOI: https://doi.org/10.1007/s10681-005-2416-3