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QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry

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Abstract

The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny ‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established.

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Abbreviations

ANOVA::

analysis of variance

bp::

base pairs

IM::

Interval Mapping

KW::

Kruskal Wallis

LG::

linkage group

LOD::

Logarithm of the odds

m/z::

mass charge ratio

ppbv::

part per billion by volume

PTR-MS::

Proton Transfer Reaction- Mass Spectrometry

QTLs::

Quantitative Trait Loci

RAPD::

Random Amplified Polymorphism DNA

SSR::

Simple Sequence Repeats

VOCs::

Volatile Organic Compounds

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Authors and Affiliations

  1. Istituto Agrario di S. Michele all'Adige, via E. Mach, 2 I-38010, S. Michele all'Adige, Italy

    Elena Zini, Franco Biasioli, Flavia Gasperi, Daniela Mott, Eugenio Aprea & Matteo Komjanc

  2. Institut für Ionenphysik, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria

    Eugenio Aprea & Tilmann D. Märk

  3. Department of Plasmaphysics, University of Bratislava, SK-84248, Bratislava, Slovak Republic

    Tilmann D. Märk

  4. Institute of Plant Sciences, Swiss Federal Institute of Technology, Universitätstrasse 2, CH-8092, Zürich, Switzerland

    Andrea Patocchi & Cesare Gessler

  5. SafeCrop center, Istituto Agrario di S. Michele all'Adige, via E. Mach, 2 I-38010, S. Michele all'Adige, Italy

    Cesare Gessler

Authors
  1. Elena Zini
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  2. Franco Biasioli
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  3. Flavia Gasperi
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  4. Daniela Mott
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  5. Eugenio Aprea
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  6. Tilmann D. Märk
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  7. Andrea Patocchi
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  8. Cesare Gessler
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  9. Matteo Komjanc
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Correspondence to Matteo Komjanc.

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The two authors contributed equally to this work.

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Zini, E., Biasioli, F., Gasperi, F. et al. QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry. Euphytica 145, 269–279 (2005). https://doi.org/10.1007/s10681-005-1645-9

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  • DOI: https://doi.org/10.1007/s10681-005-1645-9

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