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Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.)

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Cultivated peanut (Arachis hypogaea L.) consists of six botanical varieties. Identification of DNA markers associated with botanical varieties would be useful in plant genotyping, germplasm management, and evolutionary studies. We have developed 130 simple sequence repeat (SSR) markers in peanut, 38 of which were used in this study because of their ability in detecting genetic polymorphism among 24 peanut accessions. Eight SSR markers were found useful to classify botanical varieties. Among them, six SSR markers were specific to botanical varieties fastigiata and vulgaris, one to botanical varieties hypogaea and hirsuta, and one to botanical varieties peruviana, and aequatoriana. Also, three of them derived from peanut expressed sequence tags (ESTs) were associated with putative genes. As botanical varieties have different morphological traits and belong to different subspecies in A. hypogaea, these markers might be associated with genes involved in the expression of morphological traits. The results also suggested that SSRs (also called microsatellites) might play a role in shaping evolution of cultivated peanut. Multiplex PCR of botanical variety-specific markers could be applied to facilitate efficient genotyping of the peanut lines.

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  • Goldstein, D.B. & C. Schlotterer, 1999. Evolution and Applications. In: Microsatellites. Oxford University Press, New York.

    Google Scholar 

  • Guo, B.Z., M. Luo & C.C. Holbrook, 2003. Nucleotide sequences of 1345 ESTs (expressed sequence tags) (accession no. CD037499 to CD 038843) from peanut (Arachis hypogaea L.) GenBank, National Center for Biotechnology Information.

  • He, G.H. & C.S. Prakash, 2001. Evaluation of genetic relationships among botanical varieties of cultivated peanut (Arachis hypogaea L.) using AFLP markers. Genet Resour Crop Evol 48: 347–352.

    Article  Google Scholar 

  • He, G.H., R.H. Meng, M. Newman, G.Q. Gao, R.N. Pittman & C.S. Prakash, 2003. Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.). BMC Plant Biol 3: 3.

    Article  PubMed  Google Scholar 

  • Henegariu, O., N.A. Heerema, S.R. Dlouhy, G.H. Vance & P.H. Vogt, 1997. Multiplex PCR: Critical parameters and step-by-step protocol. BioTechniques 23: 504–511.

    CAS  PubMed  Google Scholar 

  • Luikart, G., M.P. Biju-Duval, O. Ertugrul, Y. Zagdsuren, C. Maudet & P. Taberlet, 1999. Power of 22 microsatellite markers in fluorescent multiplexes for parentage testing in goats Capra hircus. Anim Genet 30: 431–438.

    Article  CAS  PubMed  Google Scholar 

  • Mellersh, C. & J. Sampson, 1993. Simplifying detection of microsatellite length polymorphisms. BioTechniques 15: 582–584.

    CAS  PubMed  Google Scholar 

  • Mitchell, S.E., S. Kresovich, C.A. Jester, C.J. Hernandez & A.K. Szewe-McFadden, 1997. Application of multiplex PCR and fluorescence-based, semi-automated allele sizing technology for genotyping plant genetic resources. Crop Sci 37: 617–624.

    Article  CAS  Google Scholar 

  • Narvel, J.M., W.C. Chu, W.R. Fehr, P.B. Cregan & R.C. Shoemaker, 2000. Development of multiplex sets of simple sequence repeat DNA markers covering the soybean genome. Mol Breed 6: 175–183.

    Article  CAS  Google Scholar 

  • Scheef, E.A., M.D. Casler & G. Kung, 2003. Development of species-specific SCAR markers in bentgrass. Crop Sci 43: 345–349.

    Article  CAS  Google Scholar 

  • Stalker, H.T. & C.E. Simpson, 1995. Germplasm resources in Arachis. In H.E. Pattee & H.T. Stalker (Eds.), Advances in peanut science, Chapter 2, pp. 14–53. APRES, Inc. Stillwater, OK, USA.

    Google Scholar 

  • Tang, S., V.K. Kishore & S.J. Knapp, 2003. PCR-multiplexes for a genome-wide framework of simple sequence repeat marker loci in cultivated sunflower. Theor Appl Genet 107: 6–19.

    CAS  PubMed  Google Scholar 

  • Williams, D.E., 1989. Exploration of Amazonian Bolivia yields rare peanut landraces. Diversity 5(4): 12–13.

    Google Scholar 

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Correspondence to Guohao He.

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He, G., Meng, R., Gao, H. et al. Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.). Euphytica 142, 131–136 (2005).

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