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Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars

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Abstract

Genetic diversity is the basis for successful crop improvement and can be estimated by different methods. The objectives of this study were to estimate the genetic diversity of 30 ancestral to modern hard red winter wheat (Triticum aestivum L.) cultivars adapted to the Northern Great Plains using pedigree information, morphological traits (agronomic measurements from six environments), end-use quality traits (micro-quality assays on 50 g grain or milled flour samples for the six environments), and molecular markers (seed storage proteins separated using SDS-PAGE, 51 SSRs, and 23 SRAP DNA markers), and to determine the relationships of genetic distance estimates obtained from these methods. Relationships among diversity estimates were determined using simple (Pearson) and rank (Spearman) correlation coefficients between distance estimates and by clustering cultivars using genetic-distances for different traits. All methods found a wide range in genetic diversity. The genetic distance estimates based on pedigree had the highest values due to possible over-estimation arising from model assumptions. The genetic diversity estimates based on seed storage protein were lowest because they were the major determinants of end-use quality, which is a highly selected trait. In general, the diversity estimates from each of the methods were positively correlated at a low level with the exceptions of SRAP diversity estimates being independent of morphologic traits (simple correlation), SDS-PAGE, and SSR diversity estimates (rank correlation). However, SSR markers, thought to be among the most efficient markers for estimating genetic diversity, were most highly correlated with seed storage proteins. The procedures used to accurately estimate genetic diversity will depend largely upon the tools available to the researcher and their application to the breeding scheme.

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Abbreviations

COD:

coefficient of diversity

COP:

coefficient of parentage

GS:

genetic similarity

GD:

genetic distance

GEI:

genotype by environment interaction

HMW:

high molecular weight

LMW:

low molecular weight

SDS-PAGE:

sodium dodecyl sulfate–polyacrylamide gel electrophoresis

SSRs:

simple sequence repeats

SRAPs:

sequence-related amplified polymorphism

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Authors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA

    H. Fufa, P. S. Baenziger, B.S. Beecher & I. Dweikat

  2. USDA-ARS and Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA

    R. A. Graybosch

  3. Department of Statistics, University of Nebraska, Lincoln, NE, 68583, USA

    K. M. Eskridge

  4. USDA-ARS, E-202 Food Quality Bldg., Pullman, WA, 99164, USA

    B.S. Beecher

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  1. H. Fufa
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  2. P. S. Baenziger
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  3. B.S. Beecher
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  4. I. Dweikat
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  5. R. A. Graybosch
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  6. K. M. Eskridge
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Correspondence to H. Fufa.

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Fufa, H., Baenziger, P.S., Beecher, B. et al. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica 145, 133–146 (2005). https://doi.org/10.1007/s10681-005-0626-3

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