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Euphytica

, Volume 140, Issue 1–2, pp 107–120 | Cite as

Potential of marker-assisted selection in hemp genetic improvement

  • G. Mandolino
  • A. Carboni
Article

Summary

The development and applications of molecular markers to hemp breeding are recent, dating back only to the mid-1990s. The main achievements in this field are reviewed. The analysis of Cannabis germplasm by RAPD, AFLP and microsatellites is discussed, with its consequence for the still debated species concept in Cannabis. DNA-based markers have also been exploited in the field of forensic science, in an attempt to discriminate licit from illicit crop. The main applications of the molecular markers to the breeding, however, have been achieved with the development of markers closely linked to the male sex and to some of the most relevant chemotypes. Active research is carried out by several groups in the field of identification and characterization of the genes involved in fiber quality and quantity, and in the determination of monoecy, another very important target of hemp breeding. Besides, markers associated to new, potentially useful chemotypes are being developed, for the marker-assisted breeding of pharmaceutical Cannabis.

Key words

Cannabis breeding forensic marker-assisted selection molecular markers 

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References

  1. Alghanim, H.J. & J.R. Almirall, 2003. Development of microsatellite markers in Cannabis sativa for DNA typing and genetic relatedness analysis. Anal Bioanal Chem 376: 1225–1233.PubMedGoogle Scholar
  2. Bartsch, D., M. Lehnen, J. Clegg, M. Pohl-Orf, I. Schuphan & N. Ellstrand, 1999. Impact of gene flow from cultivated beet on genetic diversity of wild sea beet populations. Mol Ecol 8: 1733–1741.PubMedGoogle Scholar
  3. Beckmann, J.S. & M. Soller, 1983. Restriction fragment length polymorphisms in genetic improvement: Methodologies, mapping and costs. Theor Appl Genet 67: 35–43.Google Scholar
  4. Becu, D.M.S., H.D. Mastebroek & H.J.P. Marvin, 1998. Breeding for root knot nematode resistance in hemp. In: Proceedings of ‘Bast Fibrous Plants Today and Tomorrow’, 28–30 September, p. 149, St. Petersburg.Google Scholar
  5. Boudet, A.M., S. Kajita, J. Grima-Pettenati & D. Goffner, 2003. Lignin and lignocellulosics: A better control of synthesis for new and improved uses. Trends Plant Sci 8: 576–581.PubMedGoogle Scholar
  6. Brown, A.H.D. & B.S. Weir, 1983. Measuring genetic variability in plant populations. In: S.D. Tanksley, T.J. Orton, (Eds.), Isozymes in Plant Genetics and Breeding: Vol. 1A. Developments in Plant Genetics and Breedings, pp. 219–240. Elsevier, Amsterdam.Google Scholar
  7. Carboni, A., C. Paoletti, V.M.C. Moliterni, P. Ranalli & G. {Mandolino,} 2000. Molecular markers as genetic tools for hemp characterization. In: Proceedings of Bioresource Hemp, 13–18 September, Wolfsburg. On line at www.nova-institut.de/bioresource-hemp/op.htm.
  8. Carpita, N., M. Terney & M. Campbell, 2001. Molecular biology of plant cell wall: Searching the genes that define structure, architecture and dynamics. Plant Mol Biol 47: 1–5.PubMedGoogle Scholar
  9. Clark, M.S., J.S. Parker & C.A. Ainsworth, 1993. Repeated DNA and heterochromatine structure in Rumex acetosa. Heredity 70: 527–536.Google Scholar
  10. Coyle, H.M., G. Shutler, S. Abrams, J. Hanniman, S. Neylon, C. Ladd, T. Palmbach & H.C. Lee, 2003. A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis. J Forensic Sci 48: 343–347.Google Scholar
  11. de Meijer, E.P.M., M. Bagatta, A. Carboni, P. Crucitti, V.M.C. Moliterni, P. Ranalli & G. Mandolino, 2003. The inheritance of chemical phenotype in Cannabis sativa L. Genetics 163: 335–346.PubMedGoogle Scholar
  12. Di Stilio, V.S., R.V. Kesseli & D.L. Mulcahy, 1998. A pseudoautosomal random amplified polymorphic DNA marker for the sex chromosomes of Silene dioica. Genetics 149: 2057–2062.PubMedGoogle Scholar
  13. Faeti, V., G. Mandolino & P. Ranalli, 1996. Genetic diversity of Cannabis sativa germplasm based on RAPD markers. Plant Breed 115: 367–370.Google Scholar
  14. Fellermeier, M. & M.H. Zenk, 1998. Prenylation of olivetolate by a hemp transferase yields cannabigerolic acid, the precursor of tetrahydrocannabinol. FEBS Lett 427: 283–285.PubMedGoogle Scholar
  15. Fellermeier, M., W. Eisenreich, A. Bacher & M.H. Zenk, 2001. Biosynthesis of cannabinoids. Incorporation experiments with 13C-labeled glucoses. Eur J Biochem 268: 1596–1604.PubMedGoogle Scholar
  16. Flachowsky, H., E. Schumann, W.E. Weber & A. Peil, 2001. Application of AFLP for the detection of sex-specific markers in hemp. Plant Breed 120: 305–309.Google Scholar
  17. Forapani, S., A. Carboni, C. Paoletti, V.M.C. Moliterni, P. Ranalli & G. Mandolino, 2001. Comparison of hemp (Cannabis sativa L.) varieties using Random Amplified Polymorphic DNA markers. Crop Sci 41: 1682–1689.CrossRefGoogle Scholar
  18. Fournier, G. & M. Paris, 1980. Détermination de chimiotypes à partir des cannabinoïdes chez le chanvre à fibres monoïque (Cannabis sativa L.) Possibilités de sélection. Physiologie Végétale 18: 349–356.Google Scholar
  19. Fournier, G., C. Richez-Dumanois, J. Duvezin, J.-P. Mathieu & M. Paris, 1987. Identification of a new chemotype in Cannabis sativa: Cannabigerol-dominant plants, biogenetic and agronomic prospects. Planta Med 53: 277–280.PubMedGoogle Scholar
  20. Gillan, K., M.D. Cole, A. Linacre, J.W. Thorpe & N.D. Watson, 1995. Comparison of Cannabis sativa by Random Amplified of Polymorphic DNA (RAPD) and HPLC of cannabinoids: A preliminary study. Sci Justice 35: 169–177.PubMedGoogle Scholar
  21. Gilmore, S. & R. Peakall, 2003. Isolation of microsatellite markers in Cannabis sativa L. (marijuana). Mol Ecol Notes 3: 105–108.Google Scholar
  22. Gilmore, S., R. Peakall & J. Robertson, 2003. Short Tandem Repeat (STR) DNA markers are hypervariable and informative in Cannabis sativa: Implications for forensic investigations. Forensic Sci Int 131: 65–74.PubMedGoogle Scholar
  23. Hillig, K., 2004. Genetic evidence for speciation in Cannabis (Cannabaceae). Gen Res Crop Evol, in press.Google Scholar
  24. Hsieh, H.M., R.J. Hou, L.C. Tsai, C.S. Wei, S.W. Liu, L.H. Huang, Y.C. Kuo, A. Linacre & J.C.I. Lee, 2003. A highly polymorphic STR locus in Cannabis sativa. Forensic Sci Int 131: 53–58.PubMedGoogle Scholar
  25. Jagadish, V., J. Robertson & J. Gibbs, 1996. A RAPD analysis distinguished Cannabis sativa samples from different sources. Forensic Sci Int 79: 113–121.Google Scholar
  26. Jake, J., K. Kindlhofer & B. Javornik, 2001. Assessment of genetic variation and differentiation of hop genotypes by microsatellites and AFLP markers. Genome 44: 773–782.Google Scholar
  27. Kim, E.S. & P.G. Mahlberg, 2003. Secretory vescicle formation in the secretory cavity of glandular trichomes of Cannabis sativa L. (Cannabaceae). Mol Cells 15: 387–395.PubMedGoogle Scholar
  28. Kohjyouma, M., I.-J. Lee, O. Iida, K. Kurihara, K. Yamada, Y. Makino, S. Sekita & M. Satake, 2000. Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA. Biol Pharm Bull 23: 727–730.PubMedGoogle Scholar
  29. Kojoma, M., O. Iida, Y. Makino, S. Sekita, M. Satake, 2002. DNA fingerprinting of Cannabis sativa using Inter-Simple Sequence Repeat (ISSR) amplification. Planta Med 68: 60–63.PubMedGoogle Scholar
  30. Lewontin, R.C. & J.L. Hubby, 1966. A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54: 595–609.PubMedGoogle Scholar
  31. Linacre, A. & J. Thorpe, 1998. Detection and identification of cannabis by DNA. Forensic Sci Int 91: 71–76.PubMedGoogle Scholar
  32. Mandolino, G., V. Faeti, A. Carboni & P. Ranalli, 1997. A 400 bp marker tightly linked to the male phenotype in dioecious hemp. In: Proceedings of the 2nd Bioresource Hemp Symposium, 27 February–2 March, pp. 195–196, Frankfurt, Germany.Google Scholar
  33. Mandolino, G., A. Carboni, S. Forapani & P. Ranalli, 1998. DNA markers associated with sex phenotype in hemp (Cannabis sativa L.). In: Proceedings of the Bast Fibrous Plants Today and Tomorrow Meeting, 28–30 September, pp. 197–201, St. Petersburg, Russia.Google Scholar
  34. Mandolino, G., A. Carboni, S. Forapani, V. Faeti, P. Ranalli, 1999. Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.). Theor Appl Genet 98: 86–92.Google Scholar
  35. Mandolino, G., A. Carboni, M. Bagatta, V.M.C. Moliterni & P. Ranalli, 2002. Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L. Euphytica 126: 211–218.Google Scholar
  36. Mandolino, G. & P. Ranalli, 2002. The application of molecular markers in genetics and breeding of hemp. J Ind Hemp 7: 7–23.Google Scholar
  37. Mandolino, G., M. Bagatta, A. Carboni, P. Ranalli, & E.P.M. de {Meijer,} 2003. Qualitative and quantitative aspects of the inheritance of chemical phenotype in Cannabis. J Ind Hemp 8: 51–72.Google Scholar
  38. Mohan, M., S. Nair, A. Baghwat, T.G. Krishna, M. Yano, C.R. Bhatia & T. Sasaki, 1997. Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3: 87–103.CrossRefGoogle Scholar
  39. Morgante, M. & A.M. Olivieri, 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175–182.CrossRefPubMedGoogle Scholar
  40. Morris, D., 2002. Why has the hemp revolution bypassed the United States? J Ind Hemp 7: 61–65.Google Scholar
  41. Paran, I. & R.W. Michelmore, 1993. Development of PCR-based markers linked to downy mildew resistance in lettuce. Theor Appl Genet 85: 985–993.Google Scholar
  42. Peil, A., E. Schumann, H. Flachowsky, U. Kriese, M. El Ghani, M. Riedel & W.E. Weber, 2000. AFLP markers for male plants of hemp (Cannabis sativa L.). In: Proceedings of the Bioresource Hemp, 13–18 September, Wolfsburg, Germany. On line at www.nova-institut.de/bioresource-hemp/op.htm.
  43. Peil, A., H. Flachowsky, E. Schumann & W.E. Weber, 2001. In: Proceedings of the International Conference on “Bast Fibrous Plants on the Turn of the Second and Third Millennium”, 18–22 September, pp. 1–7, Shenyang, China.Google Scholar
  44. Peil, A., H. Flachowsky, E. Schumann & W.E. Weber, 2003. Sex-linked AFLP markers indicate a pseudoautosomal region in hemp (Cannabis sativa L.). Theor Appl Genet 107: 102–109.PubMedGoogle Scholar
  45. Rick, C.M. & J. Fobes, 1974. Association of an allozyme with nematode resistance. Tomato Genet Coop Rep 24, 25.Google Scholar
  46. Sakamoto, K., K. Shimomura, Y. Komeda, H. Kamada & S. Satoh, 1995. A male-associated DNA sequence in a dioecious plant, Cannabis sativa L. Plant Cell Physiol 36: 1549–1554.PubMedGoogle Scholar
  47. Sakamoto, K., Y. Akiyama, K. Fukui, H. Kamada & S. Satoh, 1998. Characterization, genome sizes and morphology of sex chromosomes in hemp (Cannabis sativa L.). Cytologia 63: 459–464.Google Scholar
  48. Sakamoto, K., N. Ohmido, K. Fukui, H. Kamada & S. Satoh, 2000. Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa. Plant Mol Biol 44: 723–732.PubMedGoogle Scholar
  49. Shao, H., S.-J. Song & R.C. Clarke, 2003. Female-associated DNA polymorphisms of hemp (Cannabis sativa L.). J Ind Hemp 8: 5–9.Google Scholar
  50. Siniscalco Gigliano, G., P. Caputo & S. Cozzolino, 1997. Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest. Sci Justice 37: 171–174.CrossRefPubMedGoogle Scholar
  51. Siniscalco Gigliano, G. & A. Di Finizio, 1997. The Cannabis sativa L. fingerprint as a tool in forensic investigations. Bull Narcotics 1: www.unodc.org/unodc/en/bulletin/bulletin_1997-01-01_1_page007.html.
  52. Small, E. & H.D. Beckstead, 1973. Common cannabinoid phenotypes in 350 stocks of Cannabis. Lloydia 36: 144–165.PubMedGoogle Scholar
  53. S.D., N.D. Young, A.H. Paterson & M.W. Bonierbale, 1989. RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7: 264.Google Scholar
  54. Taura, F., S. Morimoto & Y. Shoyama, 1995. First direct evidence for the mechanism of delta 1-tetrahydrocannabinolic acid biosynthesis. J Am Chem Soc 38: 9766–9767.Google Scholar
  55. Taura, F., S. Morimoto & Y. Shoyama, 1996. Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L. J Biol Chem 271: 17411–17416.PubMedGoogle Scholar
  56. Toth, G., Z. Gáspári & J. Jurka, 2000. Microsatellites in different eukaryotic genomes: Survey and analysis. Genome Res 10: 967–981.CrossRefPubMedGoogle Scholar
  57. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.PubMedGoogle Scholar
  58. Wilkinson, M. & A. Linacre, 2000. The detection and persistence of Cannabis sativa DNA on skin. Sci Justice 40: 11–14.PubMedGoogle Scholar
  59. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531– 6535.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Istituto Sperimentale per le Colture IndustrialiBolognaItaly

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