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Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture

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Abstract

Using three varieties of Brassica rapa, cv. Hauarad (accession 708), cv. Maoshan-3 (714) and cv. Youbai (715), as the maternal plants and one variety of B. oleracea cv. Jingfeng-1 (6012) as the paternal plant, crosses were made to produce interspecific hybrids through ovary culture techniques. A better response of seed formation was observed when ovaries were cultured in vitro at 9–12 days after pollination on the basal MS and B5 media supplemented with 6-benzylaminopurine (BA) and naphthylacetic acid (NAA). The best response was observed for cross 714×6012 with the rate of seeds per ovary reaching 43.0%. Seeds for cross 715×6012 showed the best germination response (66.7%) on the regeneration medium (MS+1.0 mg l−1 BA+0.05 mg l−1 NAA). In all three cross combinations, good response in terms of root number and length of plants was observed on the root induction medium (MS+1.0 mg l−1 BA+0.1 mg l−1 NAA). A better response was observed for the regenerated plants cultured for 14 days than for 7 days. The ovary-derived plants with well-developed root system were hardened for 8 days and their survival rate reached over 80%. Cytological studies showed that the chromosome number of all plants tested was 19 (the sum of both parents), indicating that these regenerated plants were all true hybrids of B. rapa (n = 10) × B. oleracea (n = 9). The regenerated plants were doubled with colchicine treatment, and the best response in the crosses 708×6012, 714×6012 and 715×6012 was observed when treated with 170 mg l−1 colchicine for up to 30 h and their doubling frequency reached 52, 56 and 62%, respectively.

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References

  • Agnihotri, A., M.S. Lakshmikumaran, S. Prakash & V. Jagannathan, 1991. Embryo rescue of B. napus × Raphanobrassica hybrids. Cruciferae Newsletter 14–15: 92–93.

    Google Scholar 

  • Ayotte, R., P.M. Harney & M.V. Seuza, 1987. Transfer of triazine resistance from Brassica napus to B. oleracea L. I. Production of F1 hybrids through embryo rescue. Euphytica 36: 615–624.

    Google Scholar 

  • Ayotte, R., P.M. Harney & M.V. Seuza, 1989. The transfer of triazine resistance from Brassica napus L. to B. oleracea L. IV. Second and third backcross to B. oleracea and recovery of an 18-chroosome, triazine resistance BC3. Euphytica 40: 15–19.

    Google Scholar 

  • Bajaj, Y.P.S., S.K. Mahajan & K.S. Labana, 1986. Interspecific hybridization of Brassica napus and B. juncea through ovary, ovule and embryo culture. Euphytica 35: 103–109.

    Google Scholar 

  • Charne, D.G., P. Snider & L.S. Kott, 1987. Colchicine-treating rapeseed haploids. In: Crucifer Improvement Cooperative Proceedings, Crucifer Genetics Workshop IV, University of Wisconsin, Madison, USA.

    Google Scholar 

  • Chen, B.Y., W.K. Heneen & R. Jonsson, 1988. Resynthesis of Brassica napus L. through interspecific hybridization between Brassica alboglabra Bailey and B. campestris L. with special emphasis on seed colour. Plant Breeding 101: 52–59.

    Google Scholar 

  • Chen, Z.Z., S. Snyder, Z.G. Fan & W.H. Loh, 1994. Efficient production of doubled haploid plants through chromosome doubling of isolated microspores in Brassica napus. Plant Breeding 113: 217–221.

    Google Scholar 

  • Chen, S.Z., J.M. Yin, Z.L. Tang & J.N. Li, 2000. A preliminary study on interspecific hybridization between Brassica napus and B. oleracea var.aceaphala. J Southwest Agric Univ 22: 208-210.

    Google Scholar 

  • Chen, Y. & A. Wojciechowski, 2000. Obtained interspecific hybrid of B. napus and B. oleracea var. italica by embryo and ovule in vitro culture. J Huazhong Agric Univ 19: 274–278.

    Google Scholar 

  • Gamborg, O.L., R.A. Miller & K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158.

    CAS  PubMed  Google Scholar 

  • Gu, H.H., P. Hagberg & W.J. Zhou, 2004a. Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.). Plant Growth Regul 42: 137–143.

    Google Scholar 

  • Gu, H.H., D.Q. Zhang & W.J. Zhou, 2004b. Effects of medium renovation and colchicine treatment on embryogenesis of isolated microspores in Brassica rapa ssp.chinensis. Acta Agron Sinica 30: 78–81.

    Google Scholar 

  • Gu, H.H., W.J. Zhou & P. Hagberg, 2003. High frequency spontaneous production of doubled haploid plants from microspore culture in Brassica rapa ssp.chinensis. Euphytica 134: 239–245.

    Google Scholar 

  • Heath, D.W. & E.D. Earle, 1996. Resynthesis of rapeseed (Brassica napus L.): A comparison of sexual versus somatic hybridization. Plant Breed 115: 395–401.

    Google Scholar 

  • Hansen, N.J.P. & S.B. Andersen, 1996. In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin and APM in Brassica napus microspore culture. Euphytica 88: 159–164.

    Google Scholar 

  • Ji, D.P., 1990. Experiment of Genetics, pp. 3–7. China Agriculture Press, Beijing, China.

    Google Scholar 

  • Jourdan, P.S., E.D. Earle & M.A. Mutschler, 1989. Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris. Theor Appl Genet 78: 445–455.

    Google Scholar 

  • Liu, H.L, 1984. Genetics and Breeding of Oilseed Rape. Shanghai Science and Technology Press, Shanghai, China.

    Google Scholar 

  • McNaughten, I.M., 1963. The scope and problems involved in synthesizing new amphidiploid and autotetrapoid fodder brassicas in the group of B. napus L., B. campestris L. and B. oleracea L. Scottish Plant Breeding Station Record 48–68.

  • Mohapatra, D. & Y.P.S. Bajaj, 1988. Hybridization in Brassica juncea × Brassica campestris through ovary culture. Euphytica 37: 83–88.

    Google Scholar 

  • Mollers, C., M.C.M. Iqbal & G. Robbelen, 1994. Efficient production of doubled haploid Brassica napus plants by colchicine treatment of microspores. Euphytica 75: 95–104.

    Google Scholar 

  • Momotaz, A., M. Kato & F. Kakihara, 1998. Production of intergeneric hybridization between Brassica and Sinapis species by means of embryo rescue techniques. Euphytica 103: 123-130.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.

    CAS  Google Scholar 

  • Nishi, S., 1980. Differentiation of Brassica crops in Asia and the breeding of ‘hakuran’, a newly synthesized leafy vegetable. In: S.Tsunoda, K. Hinata & C. Gomez-Campo(Eds.), Brassica Crops and Wild Allies: Biology and Breeding, pp. 133–150. Japan Scientific Societies Press, Tokyo.

    Google Scholar 

  • Olsson, G. & S. Ellerstron, 1980. Polyploidy breeding in Europe. In: S.Tsunoda, K. Hinata & C. Gomez-Campo(Eds.), Brassica Crops and Wild Allies, pp. 167–190. Japan Science Society Press, Tokyo, Japan.

    Google Scholar 

  • Quazi, M.H., 1988. Interspecific hybrids between Brassica napus L. and B. oleracea L. developed by embryo culture. Theor Appl Genet 75: 309–318.

    Google Scholar 

  • Ozminkowski, R.H., Jr. & P.S. Jourdan, 1993. Comparison of somatic and sexual interspecific hybridization for the development of new Brassica vegetable crops. In: J. Janick & J.E.Simon (Eds.), New Crops, pp. 565–569. Wiley, New York.

    Google Scholar 

  • Swanson, E.B., M.P. Coumans & D.J. Brown, 1988. The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep 7: 83–87.

    Google Scholar 

  • Zhang, G.Q., W.J. Zhou, H.H. Gu, W.J. Song & E.J.J. Momoh, 2003. Plant regeneration from the hybridization of Brassica napus and B. juncea through embryo culture. J Agron Crop Sci 189: 347–350.

    Google Scholar 

  • Zhang, G.Q., W.J. Zhou, X.L. Yao & Z.J. Zhang, 2001a. Studies on distant hybridization in Brassica plants. J Shanxin Agric Sci 29: 25–30.

    Google Scholar 

  • Zhang, X.W., M.Q. Gao, Y.X. Yuan, J.F. Geng, Y.C. Wen, S.F. Zhang & G.Y. Li, 2001b. Studies on artificially synthesized B. napus L. J Henan Agric Sci 2: 7–10.

    Google Scholar 

  • W.J.Zhou, 2001. Oilseed rape. In: G.P. Zhang & W.J.Zhou (Eds.), Crop Production, pp. 153–178. Zhejiang University Press, Hangzhou, China.

    Google Scholar 

  • Zhou, W.J., P. Hagberg & G.X. Tang, 2002a. Increasing embryogenesis and doubling efficiency by immediate colchicine treatment of isolated microspores in spring Brassica napus. Euphytica 128: 27–34.

    Google Scholar 

  • Zhou, W.J., B.Z. Mao, G.X. Tang & P. Hagberg, 2002b. Studies on the determination of ploidy level of microspore-derived plants in Brassica napus. Scientia Agric Sinica 35: 724–727.

    Google Scholar 

  • Zhou, W.J., G.X. Tang & P. Hagberg, 2002c. Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus. Plant Growth Regul 37: 185–192.

    Google Scholar 

  • Zhou, W.J., G.X. Tang, G.Q. Zhang & P. Hagberg, 2002d. Studies on efficient production of doubled haploid plants by colchincine treatment in microspore culture of Brassica napus. Scientia Agric Sinica 35: 410–414.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    G. Q. Zhang, G. X. Tang, W. J. Song & W. J. Zhou

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  1. G. Q. Zhang
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  2. G. X. Tang
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  3. W. J. Song
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  4. W. J. Zhou
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Correspondence to W. J. Zhou.

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Zhang, G.Q., Tang, G.X., Song, W.J. et al. Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture. Euphytica 140, 181–187 (2004). https://doi.org/10.1007/s10681-004-3034-1

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  • DOI: https://doi.org/10.1007/s10681-004-3034-1

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