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QTL analysis of seed dormancy in rice (Oryza sativa L.)

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Abstract

Effective cumulative temperature (ECT) after heading would be a more reasonable parameter for seed sampling of pre-harvest sprouting/seed dormancy (SD) tests in segregating populations than the days after flowering. SD is an important agronomic trait associated with grain yielding, eating quality and seed quality. To identify genomic regions affecting SD at different grain-filling temperatures, and to further examine the association between SD and ECT during grain-filling, 127 double haploid (DH) lines derived from a cross between ZYQ8 (indica)/JX17 (japonica) by anther culture were analyzed. The quantitative trait loci (QTLs) and their digenic epistasis for SD were identified using a molecular linkage map of this population. A total of four putative QTLs for SD (qSD-3, qSD-5, 6 and 11) were detected on chromosomes 3, 5, 6 and 11, together explaining 41.4% of the phenotypic variation. Nine pairs of digenic epistatic loci were associated with SD on all but chromosome 9, and their contributions to phenotypic variation varied from 2.87%–8.73%. The SD QTL on chromosome 3 was identical to the QTLs found in other mapping populations with different genetic backgrounds, which could be a desirable candidate for gene cloning and marker-assisted selection in rice breeding.

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Authors and Affiliations

  1. College of Life Science, Zhejiang University, Hangzhou, 310029, P.R. China

    Longbiao Guo, Ping Wu & Qian Qian

  2. State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou, 310006, P.R. China

    Longbiao Guo & Dali Zeng

  3. Institute of Genetics, China Academy of Sciences, Beijing, 100101, P.R. China

    Lihuang Zhu

  4. Department of Plant Breeding, Cornell University, Ithaca, New York, 14853-1901, U.S.A.

    Yunbi Xu

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  1. Longbiao Guo
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  2. Lihuang Zhu
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  6. Qian Qian
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Correspondence to Qian Qian.

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Guo, L., Zhu, L., Xu, Y. et al. QTL analysis of seed dormancy in rice (Oryza sativa L.). Euphytica 140, 155–162 (2004). https://doi.org/10.1007/s10681-004-2293-1

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  • DOI: https://doi.org/10.1007/s10681-004-2293-1

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