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Double-frequency impedance measurement method of determination of body hydration

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Abstract

The aim of the present study is to estimate the changes in cell hydration in different conditions using impedance measurements. Experiments were performed on adult and young male albino rats. Tissue hydration level was estimated as a difference between tissue wet and dry weight. Ouabain concentrations from 10−11 to 10−4 M, physiological solution (PS), mannit and distillated water (DW) were used. The impedance measurements were taken with “Biophys-Expert-1”. The increase in water content in the brain cortex (through the injection) raises the active conductivity after intraperitoneally injections of PS, mannit and DW. The more pronounced increase was observed in case of DW injection. The values of reactive conductivity were higher when mannit injection was made. In case of different ouabain concentrations, the active and reactive components were higher in young animals than in adult ones. Considering obtained results, the impedance measurement can be provided as a method for the estimation of tissue hydration level.

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Acknowledgments

We express our gratitude to Dr. A. Heqimyan for scientific and methodological assistance and to A. Abrahamyan for secretarial work.

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Authors and Affiliations

  1. UNESCO Chair-Life Sciences International Postgraduate Educational Center, 31 Acharyan St., 0040, Yerevan, Armenia

    Anush Deghoyan, Robert Simonyan & Sinerik Ayrapetyan

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  1. Anush Deghoyan
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  2. Robert Simonyan
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  3. Sinerik Ayrapetyan
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Correspondence to Sinerik Ayrapetyan.

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Deghoyan, A., Simonyan, R. & Ayrapetyan, S. Double-frequency impedance measurement method of determination of body hydration. Environmentalist 32, 222–227 (2012). https://doi.org/10.1007/s10669-011-9377-y

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