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Decontamination of anthracene-polluted soil through white rot fungus-induced biodegradation

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Abstract

This study investigated the effect of inoculation of white rot fungus, Pleurotus ostreatus, incubation time, and levels of contamination on anthracene degradation in contaminated soil over a 90-day period. Control samples were set up to compare rates of degradation at different levels of anthracene contamination in soil. Using HPLC, extracts from soil were analyzed on intervals of 30 days to determine the level of anthracene remaining in soil. After 90 days of incubation, it was observed that time, level of contamination, and fungal treatment affected the rate of degradation of all levels of anthracene contamination, inoculated soil showed more degradation of anthracene (76–89%) compared to control soil (33–51%). It was also observed that concentration of the anthracene increased at different sampling dates in some of the soil samples. In this study, such increase in anthracene concentration was attributed to errors of extraction, sorption of the anthracene to soil particles, and possible repolymerization of anthracene in soil organic matter. The release of ligninolytic enzymes such as lignin peroxidase, laccase, and manganese peroxidase by Pleurotus ostreatus was associated with the anthracene degradation observed. A general observation indicates that an interaction between time, level of contamination, and soil treatment is vital in the degradation of high levels of anthracene contamination by the white rot fungus.

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Authors and Affiliations

  1. Department of Civil and Environmental Engineering, University of North Carolina, Charlotte, NC, USA

    Humphrey O. Zebulun & Hilary Inyang

  2. School of Agriculture & Environmental Sciences, North Carolina A & T University, Greensboro, NC, USA

    Omoanghe S. Isikhuemhen

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  1. Humphrey O. Zebulun
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  2. Omoanghe S. Isikhuemhen
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  3. Hilary Inyang
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Correspondence to Humphrey O. Zebulun.

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Zebulun, H.O., Isikhuemhen, O.S. & Inyang, H. Decontamination of anthracene-polluted soil through white rot fungus-induced biodegradation. Environmentalist 31, 11–19 (2011). https://doi.org/10.1007/s10669-010-9284-7

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