Abstract
This research was carried out to evaluate the influence of static magnetic field on the rate of apoptosis in bone marrow stem cells (BMSCs) of rats. Extracted cells were suspended in αMEM as a culture media for 48 h. After that, cells were exposed to 15 mT static magnetic field (SMF) for 5 h, incessantly with or without FeCl2. The rate of apoptosis was then assessed via flow cytometery. The results showed that either treatment with FeCl2 or exposure to SMF enhanced the rate of apoptotic cells. Moreover, cells that were treated simultaneously with FeCl2 and SMF have higher rate of apoptosis. An increase in apoptosis by 26.5% was induced by SMF alone and an increase in apoptosis by 28.2% was induced by a combination of FeCl2 and SMF, compared to their corresponding controls. The results recommended that the effects of SMF on apoptosis may be related to increment of the number of free radicals in the cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alvaro LB, Marta AS (2000) Flow cytometry in the study of cell death. Mem Inst Oswaldo Cruz, Rio de Janeiro 95:429–433
Blumenthal WC, Ricci J (1997) Effects of low-intensity AC and/or DC electromagnetic fields on cell attachment and induction of apoptosis. Bioelectromagnetics 18:264–272. doi:10.1002/(SICI)1521-186X(1997)18:3<264::AID-BEM10>3.0.CO;2-P
Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795. doi:10.1007/s000180050041
Dini L, Abbro L (2005) Bioeffects of moderate-intensity static magnetic fields on cell cultures. Micron 36:195–207. doi:10.1016/j.micron.2004.12.009
Guo M, Hay BA (1999) Cell proliferation and apoptosis. Curr Opin Cell Biol 11(6):745–752. doi:10.1016/S0955-0674(99)00046-0
Hawley TS, Hawley RG (2004) Flow cytometry protocols, 2nd edn. Humana Press, Totowa, pp 345–354
Hulbert L, Metcalfe JC (1998) Biological responses to electromagnetic fields. FASEB J 12:395–420
Jajte J, Grzegorczyk J (2002) Effect of 7 mT static magnetic field and iron ions on rat lymphocytes: apoptosis, necrosis and free radical processes. Bioelectrochemistry 57:107–111. doi:10.1016/S1567-5394(02)00059-2
Jajte J, Grzegorczyk J, Zmyslony M, Rajkowska E, Kowalska MS, Kowalski ML (2001) Influence of a 7 mT static magnetic field and irons on apoptosis and necrosis in rat blood lymphocytes. J Occup Health 43:379. doi:10.1539/joh.43.379
Kula B, Sobczak A, Kuska R (2002) Effects of electromagnetic field on free-radical processes in steelworkers. Part I: magnetic field influence on the antioxidant activity in red blood cells and plasma. J Occup Health 44:226. doi:10.1539/joh.44.226
Lacy-Hubert A, Metcalfe JC, Hesketh R (1998) Biological responses to electromagnetic fields. FASEB J 12:395
Mohtat N, Cozens FL, Hancock-Chen T (1998) Magnetic field effects on the behavior of radicals in protein and DNA environments. Photochem Photobiol 67:111–118. doi:10.1111/j.1751-1097.1998.tb05173.x
Pardal R, Clarke MF, Morrison SJ (2003) Applying the principles of stem cell biology to cancer. Natl Rev 3:1
Repacholi MH, Greenebaum B (1999) Interaction of static and extremely low frequency electric and magnetic fields with living systems: health effects and research needs. Bioelectromagnetics 20:133. doi:10.1002/(SICI)1521-186X(1999)20:3<133::AID-BEM1>3.0.CO;2-O
Reya T, Morrison SJ, Clarke MF, Weissman IL (2001) Stem cells, cancer and cancer stem cells. Nature 414:105–111. doi:10.1038/35102167
Roy C, Repacholi M (2005) Electromagnetic field and health, a WHO perspective, World Health Organization, Geneva, Switzerland. Available from: http://www.worldaluminium.org/news/montreal/roy.htm
Sobczak A, Kula B, Dancii A (2002) Effects of electromagnetic field on free-radical processes in steelworkers. Part II: magnetic field influence on vitamin A, E and selenium concentrations in plasma. J Occup Health 44:230. doi:10.1539/joh.44.230
Ulloa-Montoya F, Verfaillie CM, Hu WS (2005) Culture systems for pluripotent stem cells. J Biosci Bioeng 100(1):12–27. doi:10.1263/jbb.100.12
Yoshikawa T, Tanigawa M, Tanigawa T, Imai A, Hongo H, Kondo M (2000) Enhancement of nitric oxide generation by low frequency electromagnetic field. Pathophysiology 7:131. doi:10.1016/S0928-4680(00)00040-7
Zmyslony M, Palus J, Jajte J, Dziubaltowska E, Rajkowska E (2000) DNA damage in rat lymphocytes treated in vitro with iron cations and exposed to 7 mT magnetic fields (static or 50 HZ). Mutat Res 453:89. doi:10.1016/S0027-5107(00)00094-4
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tavasoli, Z., Abdolmaleki, P., Mowla, S.J. et al. Investigation of the effects of static magnetic field on apoptosis in bone marrow stem cells of rat. Environmentalist 29, 220–224 (2009). https://doi.org/10.1007/s10669-008-9210-4
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10669-008-9210-4