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Protective influence of Vitamins against Petroleum-induced free radical toxicity in Rabit

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Abstract

The effect of exposure to crude oil contaminated diet on the blood antioxidant defence system, lipid peroxidation, lipid profile as well as possible protective roles of vitamins E and C were studied in rabbits. Oxidative stress induction by crude oil was indicated by significantly (P < 0.05) increased lipid peroxidation and a non-significant (P < 0.05) decrease in superoxide dismutase and catalase activities. A similar pattern was also detected in the lipid profile: total cholesterol and LDL-cholesterol insignificantly (P < 0.05) increased while HDL-cholesterol and triglyceride significantly decreased relative to rabbits fed normal diet. The reciprocal relationship between HDL-cholesterol and LDL-cholesterol in addition to compromised antioxidant enzymes could predispose exposed animals to coronary heart disease. However, pre-treatment of the diet with vitamins C and E exhibited a protective role on the toxic effect of crude oil on lipid profile, lipid peroxidation as well as antioxidant enzymes. The order of protection was vitamin E + C > vitamin E > vitamin C. These observations seemed to suggest that the protective role of vitamins C and E is synergistic. The protective role of the vitamins is probably time-dependent as significant (P < 0.05) restoration of lipid profile as well as antioxidant enzymes activities to control values was effected after four weeks of exposure. It is therefore suggested that toxic effect of crude oil may be reduced by dietary supplementation of vitamins C and E.

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References

  • Achuba, F.A. and Osakwe, S.A.: 2003, ‘Petroleum Induced Free Radical Toxicity in African Catfish (Clarias gariepinus)’, Fish. Physiol. Biochem., 29(2), 97–103.

    Article  CAS  Google Scholar 

  • Amadi, A., Dickson, A.A., and Maate: 1993, ‘Remediation of Oil Polluted Soils: 1 Effect of Organic and Inorganic Nutrient Supplemention the Performance of Maize (Zea Mays)’, Water, Air Soil Pollut 66, 59–76.

    Article  CAS  Google Scholar 

  • Ben-Dand, M.: 2000, ‘Responses of River Others to Oil Contamination: A Captive Study’, River otter Alliance Fall 2000 news.

  • Beutter, E.: 1982, ‘Catalase, in E. Beutter (ed.) Red Cell Metabolism, a Manual of Biochemical Methods’, Grune and Stratton, New York, pp. 105–106.

    Google Scholar 

  • Berry, E.M.: 1992, ‘The Effect of Nutrient on Lipoprotein Susceptibility to Oxidantion’, Current Opinion Lipidol 2, 5–11.

    Article  Google Scholar 

  • Beyer, R.E.: 1994, ‘The Role of Ascorbate in Antioxidant Protection of Biomolecules: Interaction with Vitamin E and Coenzyme Q’, J Bioenery Biomemb 26, 349–358.

    Article  CAS  Google Scholar 

  • Breimer, L.H.: 1990, ‘Moleculer Mechanisms of Oxygen Radical Caranogenesis and Mutagenesis: The Role of DNA Base Damage, Mol Carcinogenesis’ 3, 188–197.

    CAS  Google Scholar 

  • Bronk, S.F., and Gores, G.F.: 1991, ‘Acidosis Protects Against Lethal Injury of Liver Simusoidal Endothelial Cell’, Hepathology 14, 150–157.

    CAS  Google Scholar 

  • Burstein, M., Scholnick, H.R., and Morfin, R.: 1970, ‘Method for the Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions’, J. lipid. Res. 11, 583–595.

    CAS  Google Scholar 

  • Buttner, G.R. and Burns, C.P.: 1996, ‘Vitamin E Slows the Rate of Free Radical Mediated Lipid Peroxidation in Cells’, Arch. Biochem. Biophy. 334(2), 261–267.

    Article  Google Scholar 

  • Chen, H. and Tappel, A.L.: 1995, ‘Protection by Vitamin E, Selecniun, Trolox C, Ascorbic Acid, Palmitate, Acetylcysteien, Coenzyme Q., Coenzyme Qio, Betra-carotene, Canthavantine, and (+) Catechin Against Oxidative Damage to Rat Blood and Tissues in Vivo’, Free Radic. Biol. Med. 18, 949–953.

    Article  CAS  Google Scholar 

  • Crapo, J.D., McCord, J.M., and Fridovic, I.: 1978, ‘Preparation and Assay of Superioxide Dismutase’, Meth. Encymol. 5, 382–393.

    Article  Google Scholar 

  • Farris, M.W.: 1991, ‘Cadmium Toxicity: Unique Cytoprotective Properties of Alpha Tocopheryl Succinate in Hepatocytes’, Toxicology 69, 63–37.

    Article  Google Scholar 

  • Gaziano, J.M. and Hennekens, C.H.: 1992 ‘Vitamin Antioxidants and Cardiovascular Disease’, Curent Opinion Lipidol 3, 291–294.

    Article  CAS  Google Scholar 

  • Glew, R.H.: 1997, ‘Lipid Metabolism 11: Pathways of Metabolisms of Special Lipids’, in T.M. Devlin (ed.) Textbook of Biochemistry with Clinical Correlations 4th edition. Wiley-Liss Inc New York, pp. 415–417.

    Google Scholar 

  • Jesseup, D.A. and Leightion, F.A.: 1996, ‘Oil Pollution and Petroleum Toxicity to Wildlife’, in (rairbroehter A Locke LN and Hoff A (Eds.) Non Infectious Disease of Wifelife. 2 ed. Iowa State University Press, Ames, Iowa, pp. 141–156.

    Google Scholar 

  • Halliwell, B.: 1994, ‘Free Radicals, Antioxidants and Human Disease Curiosity, Cause or Consequence?’ Lancet 334, 721–724.

    Article  Google Scholar 

  • Harman, D.: 1992, ‘Role of Free Radicals in Aging and Disease’, Ann New York Acad Sci 673, 126–141.

    CAS  Google Scholar 

  • Khoja, S.M. and Marzouki, Z.H.M.: 1994, ‘Effect of Vitamins C and E Intake on Plasma Lipid Concentrations in Rats’, Ann Soudi Med 14(5), 371–374.

    CAS  Google Scholar 

  • Lass, A. and Sohal, R.S.: 2000, Effect of Coezyme Q1o and Alpha-tocopherol Content of Mitochondria on Production of Superoxide Anion Radicals ‘FASEB J’ 14, 87–94.

    CAS  Google Scholar 

  • McCord, J.M. and Fridovich, I.: 1969, ‘Superoxide Dismutase and Enzymic Function for Erythrocupeine (Hemocupreine)’, J. Biol. Chem. 244, 6049–6055.

    CAS  Google Scholar 

  • Mckee, T. and Mckee, R.I.: 1999, Biochemistry 2nd edn., McGraw Hill, New York, pp. 135–238.

    Google Scholar 

  • Misra, H.P. and Fridovic, I.: 1972, ‘The Role of Superoxide Ion in the Auto-oxidation of Epinephrine and a Simple Assay for Superoxide Dismutase’, J. Biol. Chem. 247, 3170–3175.

    CAS  Google Scholar 

  • Ohkawa, H., Okishi, N., and Yogi, K.: 1979, ‘Assay for Lipid Peroxides in Animal Tissues by Thioborbituric Acid Reaction’, Anal. Biochem. 95, 351–358.

    Article  CAS  Google Scholar 

  • Onwurah, I.N.E.: 1999, ‘Lipid Peroxidation and Protein Oxidation in Azotobacter Vinelandii Exposed to Mercury, Silver, Crude Oil and Fenton Reagent’, J. Tox Subs 18(4), 167–176.

    Article  CAS  Google Scholar 

  • Ognjanovic, B.I., Pavlovic, S.Z., Maletic, S.D., Zikic, R.V., Stajn, A.S., Radojicic, R.M., Saicic, Z.S., and Petrovic, V.M.: 2003, ‘Protective Influence of Vitamin E on Antioxidant Defence System in the Blood of Rats Treated with Cadmium’, Physiol Res 52, 563–570.

    CAS  Google Scholar 

  • Patockova, J., Marhol, P., Tumova E, Krsiak, M., Rokyta, R., Stipek, S., Crkovska, J., and Andel, M.: 2003, ‘Oxidative Stress in the Brain Tissue of Laboratory Mice with Acute Post Insulin Hypoglycemia’, Physiol. Res. 52, 131–135.

    CAS  Google Scholar 

  • Pigeolet, E., Corbisler, P., Houbion, A., Lamber, D., and Michiels, C.: 1990, ‘Glutathione Peroxidases, Superoxide Dismutase and Catalase Inactivation by Peroxides and Oxygen Derived Free Radicals’, Mech. Ageing Dev. 51, 283–297.

    Article  CAS  Google Scholar 

  • Romero, F.J., Bosch-Morell, F., Romero, M.J., Jareno, E.J., and Marine, N.: 1998, ‘Lipid Peroxidation Products and Antioxidant in Human Disease’, Environ Health Perspect 106(Suppl)5, 1390–1393.

    Google Scholar 

  • Sato, K., Niki, E., and Shimasaki, H.: 1990, ‘Free Radical-Mediated Chain Oxidation of Low Density Lipoprotein and its Synergistic Inhibition by Vitamins E and C’, Arch. Biochem. Biophys. 279, 402–405.

    Article  CAS  Google Scholar 

  • Shertzer, H.G., Bannenberger, G.K., Zhu, H., Liu, R.M., and Moildeus, P.: 1994, ‘The Role of Thiol in Mitchondrial Susceptibility to Iron and Tert-butyl Hydroperoxide Mediated Toxicity in Cultured Mouse Hepatocytes’, Chem. Res. Toxicol. 7, 358–366.

    Article  CAS  Google Scholar 

  • Shika, S.C.: 1996, ‘Oxidative Stress and Role of Antioxidants in Normal and Abnormal Sperm Function’, Frontiers Biosience 1, 78–80.

    Google Scholar 

  • Siedel, J., Schlumberger, H., Klose, S., Ziegenhorn, J., and Wahlefeld, A.W.: 1981. ‘Improved Reagent for the Enzymatic Determination of Serum Cholesterol’, J. Clin. Chem. Clin. Biochem. 19, 838–847.

    Google Scholar 

  • Sies, H.: 1991, ‘Oxidative Stress: Introeduction’, in Oxidative Stress: Oxidant and Antioxidants. Academic press, San Diego. pp. 15–22.

    Google Scholar 

  • Souza, M.F., Tome, A.R., and Rao, V.S.: 1999, ‘Inhibition by the Bioflavonoid Ternation on Aflatoxin B1-idncued Lipid Peroxidation in Rat Liver’, J. Pharm. Pharmacol. 51, 125–129.

    Article  CAS  Google Scholar 

  • Tanaka, K., Hashimoto, T., Tokumara, S., Iguchi, H., and Kojo, S.: 1997, ‘Interactions Between Vitamin C and Vitamin E are Observed in Tissues of Inherently Scorbutic Rats’, J. Nutr. 127, 2060–2064.

    CAS  Google Scholar 

  • Trush, M.A. and Kensler, T.W.: 1991, ‘An Overview of the Relationship Between Oxidative Stress and Chemical Carcinogenesis’ Free Radical Bio Med 10, 201–209.

    Article  CAS  Google Scholar 

  • Tsuchihashi, M.: 1923, Zur Kenntnis der Blutkatalase Biochem Z 140, 65–74.

    Google Scholar 

  • Val, A.L. and Almeida-Val, V.F.: 1999, ‘Effect of Crude Oil on Respiratory Aspect of Some Fish Species of the Amazon’, in A.L. Val and V. M. F. Almeida-Val (eds.), Biology of Tropical Fish, Manaus Brasil. pp. 227–291.

    Google Scholar 

  • Verma, R.J. and Nair, A.: 2001, ‘Ameliorative Effect of Vitamin E on Aflatoxin Induced Lipid Peroxidation in the Testis of Mice Asian’, J Androl 3, 217–221.

    CAS  Google Scholar 

  • Wahlefeld, A.W.: 1974, ‘Triglycerides Determination after Enzymatic Hydrolysis’. in methods of enzymatic analysis in 2nd ed. H.V. Bergemeyer (ed.) Verlag Chemi/Academic Press, New York, pp. 1831–1935.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Delta State University, P. M. B. 1, Abraka, Nigeria

    Fidelis I. Achuba

  2. Department of Chemistry, Delta State University, P.M.B. 1, Abraka, Nigeria

    Edga O. Otuya

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  1. Fidelis I. Achuba
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  2. Edga O. Otuya
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Correspondence to Fidelis I. Achuba.

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Achuba, F.I., Otuya, E.O. Protective influence of Vitamins against Petroleum-induced free radical toxicity in Rabit. Environmentalist 26, 295–300 (2006). https://doi.org/10.1007/s10669-006-0158-y

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