Abstract
The phenomenon of simultaneous nitrification and denitrification (SND) occurring inside thick biofilm in aerated reactors can be expressed in terms of simple mathematical equations based on Fick’s theory of diffusion. The fundamental concept behind the model is the formation of a diffusional gradient of substrates and dissolved oxygen within the biofilm, which creates stratified environments favoring the occurrence of two completely different processes. A simplified mathematical model is developed to analyze nitrogen removal via SND in moving bed bioreactors (MBBR), which considers the effects of biomass loss due to hydraulic shear, abrasion due to particle-to-particle collision, and endogenous decay. To estimate substrate removal in the biofilm, Monod growth kinetics is followed, and relevant boundary conditions are applied to obtain a solution using the second order differential equation. A computer program in conventional FORTRAN language is developed to predict effluent concentrations, implying that the same could be developed in other advanced languages. Experimental validation of the model confirms that analytically obtained data remain within a maximum deviation of 10%, and the total biofilm thickness is indicative of the formation of anoxic zones that support denitrification.
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References
Pynaert, K., Smets, B.F., Wyffels, S., Beheydt, D., Siciliano, S.D., & Verstraete, W. (2003). Characterization of an autotrophic nitrogen-removing biofilm from a highly loaded lab-scale rotating biological contactor. Applied and environmental microbiology, 69, 3626–3635. https://doi.org/10.1128/AEM.69.6.3626-3635.2003
Madigan, M. T., & Martinko, J. M. (2006). Microorganisms and microbiology. Brock biology of microorganisms.11th ed. Upper Saddle River, New Jersey (NJ): Pearson Prentice Hall, 1–20.
Lawrence, A. W., & McCarty, P. L. (1970). Unified basis for biological treatment design and operation. Journal of the Sanitary Engineering Division, 96,757–778. https://doi.org/10.1061/JSEDAI.0001126
Strand, S. E., & McDonnell, A. J. (1985). Mathematical analysis of oxygen and nitrate consumption in deep microbial films. Water Research, 19, 345–352. https://doi.org/10.1016/0043-1354(85)90095-8
Strand, S. E., McDonnell, A. J., & Unz, R. F. (1985). Concurrent denitrification and oxygen uptake in microbial films .Water Research, 19, 335–344. https://doi.org/10.1016/0043-1354(85)90094-6
Zeng, R. J., Lemaire, R., Yuan, Z., & Keller, J. (2003). Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor. Biotechnology and bioengineering, 84, 170–178. https://doi.org/10.1002/bit.10744
Jimenez, J., Dursun, D., Dold, P., Bratby, J., Keller, J., & Parker, D. (2010). Simultaneous nitrification-denitrification to meet low effluent nitrogen limits: Modeling, performance and reliability. Proceedings of the Water Environment Federation, 2010, 2404–2421.
Pochana, K., & Keller, J. (1999). Study of factors affecting simultaneous nitrification and denitrification (SND). Water Science and Technology, 39, 61–68. https://doi.org/10.1016/S0273-1223(99)00123-7
Bhattacharya, R., & Mazumder, D. (2021). Evaluation of nitrification kinetics for treating ammonium nitrogen enriched wastewater in moving bed hybrid bioreactor. Journal of Environmental Chemical Engineering, 9, 104589.https://doi.org/10.1016/j.jece.2020.104589
Kappeler, J., & Gujer, W. (1994). Development of a mathematical model for aerobic bulking. Water Research, 28, 303–310. https://doi.org/10.1016/0043-1354(94)90268-2
Gonzalez-Gil, G., Seghezzo, L., Lettinga, G., & Kleerebezem, R. (2001). Kinetics and mass-transfer phenomena in anaerobic granular sludge. Biotechnology and Bioengineering, 73, 125–134. https://doi.org/10.1002/bit.1044
Tartakovsky, B., & Guiot, S. R. (2004). Biofilm Modelling. Fundamentals of Cell Immobilisation Biotechnology (pp. 531–545). Springer.
RaoBhamidimarri, S. M., & See, T. T. (1992). Shear loss characteristics of an aerobic biofilm. Water Science and Technology, 26, 595–600. https://doi.org/10.2166/wst.1992.0439
Henze, M., Gujer, W., Mino, T., & van Loosdrecht, M. C. (2000). Activated sludge models ASM1, ASM2, ASM2d and ASM3. IWA publishing.
Pochana, K., Keller, J., & Lant, P. (1999). Model development for simultaneous nitrification and denitrification. Water Science and Technology, 39, 235–243. https://doi.org/10.1016/S0273-1223(98)00789-6
Daigger, G. T., & Littleton, H. X. (2014). Simultaneous biological nutrient removal: A state-of-the-art review. Water Environment Research, 86, 245–257. https://doi.org/10.2175/106143013X13736496908555
Daigger, G. T., Adams, C. D., & Steller, H. K. (2007). Diffusion of oxygen through activated sludge flocs: Experimental measurement, modeling, and implications for simultaneous nitrification and denitrification. Water environment research, 79, 375–387.https://doi.org/10.2175/106143006X111835
Layer, M., Villodres, M. G., Hernandez, A., Reynaert, E., Morgenroth, E., & Derlon, N. (2020). Limited simultaneous nitrification-denitrification (SND) in aerobic granular sludge systems treating municipal wastewater: Mechanisms and practical implications. Water research, 7, 100048. https://doi.org/10.1016/j.wroa.2020.100048
Halling-Sørensen, B., & Nielsen, S. N. (1996). A model of nitrogen removal from waste water in a fixed bed reactor using simultaneous nitrification and denitrification (SND). Ecological modelling, 87, 131–141. https://doi.org/10.1016/0304-3800(95)00025-9
Sarioglu, M., Insel, G., Artan, N., & Orhon, D. (2009). Model evaluation of simultaneous nitrification and denitrification in a membrane bioreactor operated without an anoxic reactor. Journal of Membrane Science, 337, 17–27. https://doi.org/10.1016/j.memsci.2009.03.015
He, S. B., Xue, G., & Wang, B. Z. (2009). Factors affecting simultaneous nitrification and de-nitrification (SND) and its kinetics model in membrane bioreactor. Journal of hazardous materials, 168, 704–710. https://doi.org/10.1016/j.jhazmat.2009.02.099
Seifi, M., & Fazaelipoor, M. H. (2012). Modeling simultaneous nitrification and denitrification (SND) in a fluidized bed biofilm reactor. Applied Mathematical Modelling, 36, 5603–5613. https://doi.org/10.1016/j.apm.2012.01.004
Insel, G., Hocaoğlu, S. M., Cokgor, E. U., & Orhon, D. (2011). Modelling the effect of biomass induced oxygen transfer limitations on the nitrogen removal performance of membrane bioreactor. Journal of membrane science, 368, 54–63. https://doi.org/10.1016/j.memsci.2010.11.003
Sarioglu, M., Insel, G., Artan, N., & Orhon, D. (2008). Modelling of long-term simultaneous nitrification and denitrification (SNDN) performance of a pilot scale membrane bioreactor. Water Science and Technology, 57, 1825–1833. https://doi.org/10.2166/wst.2008.121
Orhon, D., & Artan, N. (1994). Modeling of activated sludge systems. Technomic Publ. Co.
Baek, S. H., & Kim, H. J. (2013). Mathematical model for simultaneous nitrification and denitrification (SND) in membrane bioreactor (MBR) under low dissolved oxygen (DO) concentrations. Biotechnology and bioprocess engineering, 18, 104–110. https://doi.org/10.1007/s12257-011-0419-6
Zinatizadeh, A. A. L., & Ghaytooli, E. (2015). Simultaneous nitrogen and carbon removal from wastewater at different operating conditions in a moving bed biofilm reactor (MBBR): Process modeling and optimization. Journal of the Taiwan Institute of Chemical Engineers, 53, 98–111. https://doi.org/10.1016/j.jtice.2015.02.034
Williamson, K., & McCarty, P. L. (1976). A model of substrate utilization by bacterial films. Journal (Water Pollution Control Federation), 9–24.
Metcalf, E. E., & Eddy, H. (2003). Wastewater engineer treatment disposal, reuse (4th ed.). McGraw Hill Publishers.
Rittmann, B. E., & McCarty, P. L. (1980). Model of steady-state-biofilm kinetics. Biotechnology and bioengineering, 22, 2343–2357. https://doi.org/10.1002/bit.260221110
Sarkar, S., & Mazumder, D. (2017). Development of a simplified biofilm model. Water Science and Technology, 7, 1799–1806. https://doi.org/10.1007/s13201-015-0353-4
Trulear, M. G., & Characklis, W. G. (1982). Dynamics of biofilm processes. Journal (Water Pollution Control Federation), 1288–1301.
Rittman, B. E. (1982). The effect of shear stress on biofilm loss rate. Biotechnology and Bioengineering, 24, 501–506.
Goswami, S., & Mazumder, D. (2019). Modelling and process design of Moving Bed Bioreactor (MBBR) for wastewater treatment—A review. Journal of the Indian Chemical Society, 96, 215–229.
Spielman, L. A. (1978). Hydrodynamic aspects of flocculation. The scientific basis of flocculation. 63–88. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9938-1_4
Gjaltema, A., Van Der Marel, N., Van Loosdrecht, M. C. M., & Heijnen J. J. (1997). Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics. Biotechnology and Bioengineering, 55, 880–889. https://doi.org/10.1002/(SICI)1097-0290(19970920)55:63.0.CO;2-C
Beverloo, W. A., & Tramper, J. (1994). Intensity of microcarrier collisions in turbulent flow. Bioprocess Engineering, 11, 177–184. https://doi.org/10.1007/BF00369627
Zhu, S., & Chen, S. (2002). The impact of temperature on nitrification rate in fixed film biofilters. Aquacultural Engineering, 26, 221–237. https://doi.org/10.1016/S0144-8609(02)00022-5
Eaton, A. D., Clesceri, L. S., Rice, E. W., Greenberg, A. E., & Franson, M. A. H. A. (2005). APHA: standard methods for the examination of water and wastewater. Centennial Edition., APHA, AWWA, WEF, Washington, DC.
Herbert, D., Phipps, P. J., & Strange, R. E. (1971). Chapter III Chemical analysis of microbial cells. In Methods in microbiology, 5, 209–344.Academic press.
Lin, Y. H., & Gu, Y. J. (2020). Denitrification kinetics of nitrate by a heterotrophic culture in batch and fixed-biofilm reactors. Processes, 8, 547.https://doi.org/10.3390/pr8050547
Bhattacharya, R., & Mazumder, D. (2021). Simultaneous nitrification and denitrification in moving bed bioreactor and other biological systems. Bioprocess and Biosystems Engineering, 44, 635–652. https://doi.org/10.1007/s00449-020-02475-6
San Diego-McGlone, M. L., Smith, S. V., & Nicolas, V. F. (2000). Stoichiometric interpretations of C: N: P ratios in organic waste materials. Marine Pollution Bulletin, 40, 325–330. https://doi.org/10.1016/S0025-326X(99)00222-2
Liu T., He X., Jia G., Xu J., Quan X., & You S. (2020). Simultaneous nitrification and denitrification process using novel surface-modified suspended carriers for the treatment of real domestic wastewater. Chemosphere, 247, 125831. https://doi.org/10.1016/j.chemosphere.2020.125831
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Both authors have contributed towards the study’s conception and design. Manuscript preparation and experimental data collection were performed by Roumi Bhattacharya under the supervision of Debabrata Mazumder. Data analysis was done by both authors. The first draft of the manuscript was written by Roumi Bhattacharya. Both authors read and approved the final manuscript.
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Bhattacharya, R., Mazumder, D. Development of a Simplistic Mathematical Model for Simultaneous Nitrification and Denitrification in Moving Bed Bioreactor. Environ Model Assess 28, 635–650 (2023). https://doi.org/10.1007/s10666-023-09874-5
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DOI: https://doi.org/10.1007/s10666-023-09874-5