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Assessment of pollution with biomarkers in fish culture

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Abstract

Biotransformation enzymes are stimulated or inhibited depending on the exposure to xenobiotics and widely used as a biomarker. In this study, EROD activity, GST activity, reduced GSH amount, amount of lipid peroxidation product MDA as oxidative stress parameter, and ACHE inhibition as a neuromuscular parameter were measured in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss) produced in aquaculture of Kemer Dam and in concrete ponds located in Fethiye. Samples were collected in January and July from fish farming in net cages in Kemer Dam and concrete cages in Fethiye in order to evaluate seasonal changes. Also, physicochemical properties of water samples collected from trout farms were measured and evaluated together with five biomarkers. Significant ACHE inhibition was observed in both stations in parallel with the increase in ammonium nitrogen in the July period (p ≤ 0.05). In parallel with the increase in turbidity in the Kemer Dam, a significant increase in GSH, MDA level, and GST activity was detected (p ≤ 0.05). As a result of the assessment of all data, significant increases were detected in EROD induction, GST activity, MDA level, and ACHE inhibition in concrete cages in Fethiye when compared to Kemer Dam (p ≤ 0,05). It was observed that pollution was at a lower level in July compared to January, and in Kemer Dam compared to Fethiye.

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Acknowledgements

We would like to thank head of biochemistry department Prof. Dr. Funda KIRAL, faculty members Prof. Dr. Pınar Alkım ULUTAŞ and Dr. Serap ÜNÜBOL AYPAK, head of pharmacology and toxicology department Prof. Dr. Ferda AKAR, research assistants Gamze TOSUN, Gamze Servi EKREN, and central laboratory officer Necati GÜNAY at Adnan Menderes University.

Funding

This research was funded by scientific research project funding (BAP) of Adnan Menderes University.

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  1. Faculty of Veterinary Medicine, Department of Biochemistry, Adnan Menderes University, Aydın, Turkey

    Mehmet Ersin Kartal & Ayşegül Bildik

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  1. Mehmet Ersin Kartal
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Correspondence to Mehmet Ersin Kartal.

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Kartal, M.E., Bildik, A. Assessment of pollution with biomarkers in fish culture. Environ Monit Assess 194, 111 (2022). https://doi.org/10.1007/s10661-022-09774-3

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