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Toxic effects of shale gas fracturing flowback fluid on microbial communities in polluted soil

Environmental Monitoring and Assessment volume 193, Article number: 786 (2021) Cite this article

Abstract

A large amount of shale gas fracturing flowback fluid (FFBF) from the process of shale gas exploitation causes obvious ecological harm to health of soil and water. However, biological hazard of soil microbial populations by fracturing flowback fluid remains rarely reported. In this study, the microbiological compositions were assessed via analyzing diversity of microbial populations. The results showed significant differences between polluted soil by fracturing flowback fluid and unpolluted soil in different pH and temperature conditions. And then, the microbe-index of biological integrity (M-IBI) was used to evaluate the toxicity of the fracturing flowback fluid based on analysis of microbial integrity. The results showed that polluted soil lacks key microbial species known to be beneficial to soil health, including denitrifying bacteria and cellulose-decomposing bacteria, and 35 °C is a critical value for estimating poor and sub-healthy level of damage to microbial integrity by fracturing flowback fluid. Our results provide a valuable reference for the evaluation of soil damage by fracturing flowback fluid.

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Data availability

The data or materials used in this article are part of the ongoing research work of the first author, hence not to be shared until its completion.

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Acknowledgements

We appreciate the cooperation of Xi Lin, Yuan Luo, and WanLi Pu from Yangtze Normal University to our research activities.

Funding

The authors would like to thank Chongqing Natural Science Foundation project (Grant Nos. cstc2019jcyj-msxm0749), Chongqing technology Innovation and Application Demonstration Project (Grant Nos. cstc2018jszx-zdyfxmX0011), Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province (ZJKLAT2019-001), Chongqing Science Committee, Key laboratory of Degraded and Unused Land Consolidation Engineering, and the Ministry of Natural and Resources (Grant No. SXDJ2019) for funding this study.

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Authors and Affiliations

  1. Chongqing Environmental Protection Engineering Technology Center for Shale Gas Development, Fuling, 408000, People’s Republic of China

    Xudong Mei, Fanhai Zeng & FengLin Xu

  2. Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, 266 Fangzheng Avenue, Shuitu High-tech Park, Beibei, Chongqing, 400714, People’s Republic of China

    HaiFeng Su

  3. Key Laboratory of Degraded and Unused Land Consolidation Engineering, the Ministry of Natural and Resources, XiAn, ShanXi province, 710075, People’s Republic of China

    HaiFeng Su

  4. Zhejiang A&F University, No.666 Wusu Street, Lin’an District, Hangzhou, Zhejiang, 311300, People’s Republic of China

    HaiFeng Su

Authors
  1. Xudong Mei
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  2. Fanhai Zeng
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Contributions

HFS participated in the conception, design, data collection, and analysis and drafted the manuscript. XDM and FHZ assisted the laboratory work, results’ interpretation, and manuscript revision. FLX participated in the result discussion and manuscript revision. All authors read and approved the final manuscript.

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Correspondence to HaiFeng Su.

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Mei, X., Zeng, F., Xu, F. et al. Toxic effects of shale gas fracturing flowback fluid on microbial communities in polluted soil. Environ Monit Assess 193, 786 (2021). https://doi.org/10.1007/s10661-021-09544-7

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  • DOI: https://doi.org/10.1007/s10661-021-09544-7

Keywords

  • Microbe-index of biological integrity
  • Permeability
  • Microorganism evaluation
  • Soil microorganism
  • Toxic factor