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Development of an in vivo acute bioassay using the marine medaka Oryzias melastigma

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Abstract

To determine whether the marine medaka Oryzias melastigma is a suitable model organism for in vivo acute toxicity bioassay in seawater, we first determined whether there were differences in the concentrations of chemicals that were toxic to marine medaka (O. melastigma) and freshwater medaka (O. latipes). We performed in vivo acute toxicity bioassay with 3-chloroaniline, triclosan, 3,4-dichloroaniline, fenitrothion, and pyriproxyfen on larvae of both species. Although the concentrations of 3-chloroaniline and fenitrothion that were lethal to the larvae were identical for both species, the toxic concentrations of triclosan, 3,4-dichloroaniline, and pyriproxyfen were lower for O. melastigma than for O. latipes. We then used an in vivo acute toxicity bioassay to monitor the quality of coastal seawater in Akita, Japan. No lethal effects were observed in the harbor and canal in 2019. O. melastigma could be used to monitor the quality of seawater with salinities in the range 2–25. Our findings suggest that O. melastigma can be used as the test fish for in vivo acute toxicity bioassay intended for water quality monitoring.

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Funding

This study was supported partly by the Salt Science Research Foundation, Japan (grant nos. 1915 and 2021) and a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Grant-in-Aid for Scientific Research [B] grant no. 19H04294) to Y.H.

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Authors and Affiliations

  1. Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Nakano Shimoshinjo, Akita, 010-0195, Japan

    Yoshifumi Horie & Chiho Takahashi

  2. Research Center for Inland Sea (KURCIS), Kobe University, Fukae Minami Kobe, Hyogo, 658-0022, Higashinada, Japan

    Yoshifumi Horie

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  1. Yoshifumi Horie
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  2. Chiho Takahashi
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Contributions

Yoshifumi Horie, all experiment except sampling for in vivo acute bioassays; Chiho Takahashi, sampling for in vivo acute bioassays. All authors listed on the current study contributed to the experimental design or data analysis.

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Correspondence to Yoshifumi Horie.

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The fish which was used in the present study were handled according to guidelines of Akita Prefectural University.

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This research did not involve human subjects, so clinical trial registration is not applicable.

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Horie, Y., Takahashi, C. Development of an in vivo acute bioassay using the marine medaka Oryzias melastigma. Environ Monit Assess 193, 725 (2021). https://doi.org/10.1007/s10661-021-09527-8

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