Ecotoxicological evaluation of two anti-dandruff hair shampoos using Lemna minor

Abstract

Hair shampoos, a mixture of various organic and organic compounds, are commonly used personnel care products. Since shampoos are used in almost every household and beauty shop, their ingredients are common components of domestic and municipal wastewater. However, studies on the effect of shampoos to aquatic plants can hardly be found in literature. Therefore, the present study was conducted to investigate the phytotoxic effects of two commonly used anti-dandruff shampoos (named here AD 1 and AD 2) using Lemna minor as a biotest organism. For toxicity assessment, frond number, fresh and dry biomass, and light-harvesting pigments (chlorophyll a, b and total carotenoids) of Lemna were used as end points. Five different concentrations (0.001, 0.01, 0.1, 1, and 5%) of each shampoo were tested in comparison to the control. At lower concentrations of shampoos, some minor and non-significant stimulatory effects were observed in some parameters, but at concentrations above 0.01% both the shampoos significantly inhibited almost all parameters in Lemna. The EC50 values obtained for frond number were 0.034 and 0.11% for AD 1 and AD 2, respectively. The fresh biomass gave EC50 values of 0.07 and 0.066% for AD 1 and AD 2, respectively. Based on the preset study, it can be speculated that shampoo contamination at higher concentrations in water bodies can be a threat to aquatic organisms. This study can be used as a baseline to further investigate shampoo toxicity using other species and to explore the mechanism of shampoo toxicity in aquatic plants.

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References

  1. Aizdaicher, N. A., & Markina, Z. V. (2006). Toxic effects of detergents on the alga Plagioselmis prolonga (Cryptophyta). Russian Journal of Marine Biology, 32, 45–49.

    CAS  Article  Google Scholar 

  2. Aleavia. (2012). Are the products in your home dangerous? URL: Special Health Report http://aleavia.com/uploads/newsletteraleavia3reduced.pdf. Accessed January 15, 2015.

    Google Scholar 

  3. Azizullah, A., Richter, P., & Häder, D.-P. (2011). Toxicity assessment of a common laundry detergent using the freshwater flagellate Euglena gracilis. Chemosphere, 84, 1392–1400.

    CAS  Article  Google Scholar 

  4. Azizullah, A., Richter, P., Ullah, W., Ali, I., & Häder, D.-P. (2013). Ecotoxicity evaluation of a liquid detergent using the automatic biotest ECOTOX. Ecotoxicology, 22, 1043–1052.

    CAS  Article  Google Scholar 

  5. Bhatti, A. M., & Nasu, S. (2010). Domestic water demand forecasting and management under changing socio-economic scenario. Society for Social Management Systems, Japan, SMS-10-183.

  6. Chawla, G., Viswanathan, P. N., & Devi, S. (1987). Biochemical studies on the toxicity of linear alkylbenzene sulphonate to Scenedesmus quadricauda in culture. Environmental and Experimental Botany, 27, 311–319.

    CAS  Article  Google Scholar 

  7. Danilov, R., & Ekelund, N. (2000). Applicability of growth rate, cell shape, and motility of Euglena gracilis as physiological parameters for bioassessment at lower concentrations of toxic substances: An experimental approach. Environmental Toxicology, 16, 78–83.

    Article  Google Scholar 

  8. Danilov, R. A., & Ekelund, N. G. A. (2001). Effects of Cu2+, Ni2+, Pb2+, Zn2+ and pentachlorophenol on photosynthesis and motility in Chlamydomonas reinhardtii in short-term exposure experiments. BMC Ecology, 1, 1.

    CAS  Article  Google Scholar 

  9. Dvorak, V., Caldova, J., & Trnkova, L. (2012). Differential sensitivity of the Lemnaceae species to chromium and zinc. Proceedings of ECOpole, 6(2), 505–510.

    CAS  Google Scholar 

  10. Finkel, R., Cubeddu, L. X., & Clark, M. A. (2009). Pharmacology (4th ed.). Baltimore: Lippincott Williams & Wilkins.

    Google Scholar 

  11. Hou, W., Chen, X., Song, G., Wang, Q., & Chang, C. C. (2007). Effects of copper and cadmium on heavy metal polluted water body restoration by duckweed (Lemna minor). Plant Physiology and Biochemistry, 45, 62–69.

    CAS  Article  Google Scholar 

  12. Jennings, J. R. (1979). The effect of cadmium and lead on the growth of two species of marine phytoplankton with particular reference to the development of tolerance. Journal of Plankton Research, 1, 121–136.

    CAS  Article  Google Scholar 

  13. Kagalwala, A. Y., & Kavitha, K. (2012). Effects of surfactant (sodium lauryl sulphate) on Hydrilla verticillata. International Journal of Life Sciences and Biotechnology and Pharma Research, 1(2), 129–138.

    Google Scholar 

  14. Keppeler, E. C. (2009). Toxicity of sodium chloride and methyl parathion on the macrophyte Lemna minor (Linnaeus, 1753) with respect to frond number and chlorophyll. Biotemas, 22(3), 27–33.

    Google Scholar 

  15. Khan, J. A. (2011). Marketing plan 1. http://www.scribd.com/doc/72244711/Marketing-Plan-1#scribd. Accessed January 15, 2015.

  16. Leung, S., Gironella, A., Trigo, C., Bhushan, A., Daniels, C. K., & Lai, J. C. K. (2007). Cationic surfactants and other factors that affect enzymatic activities and transport. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 221(2), 153–160.

    CAS  Article  Google Scholar 

  17. Margot, J., Rossi, L., Barry, D. A., & Holliger, C. (2015). A review of the fate of micropollutants in wastewater treatment plants. Wiley Interdisciplinary Reviews: Water, 2, 457–487.

    CAS  Article  Google Scholar 

  18. Markina, Z. V. (2010). Effects of sodium dodecyl sulfate on the growth dynamics and physiological state of the microalga Dunaliella salina (Chlorophyta). Russian Journal of Marine Biology, 36, 191–194.

    CAS  Article  Google Scholar 

  19. Markina, Z. V., & Aizdaicher, N. A. (2007). Influence of laundry detergents on the abundance dynamics and physiological state of the benthic microalga Attheya ussurensis (Bacillariophyta) in laboratory culture. Russian Journal of Marine Biology, 33, 391–398.

    Article  Google Scholar 

  20. Mohan, B. S., & Hosetti, B. B. (1997). Potential phytotoxicity of lead and cadmium to Lemna minor grown in sewage stabilization ponds. Environmental Pollution, 98, 233–238.

    CAS  Article  Google Scholar 

  21. Mohan, B. S., & Hosetti, B. B. (1999). Aquatic plants for toxicity assessment. Environmental Research, 81, 259–274.

    CAS  Article  Google Scholar 

  22. Moya, I., Silvestri, M., Vallon, O., Cinque, G., & Bassi, R. (2001). Time-resolved fluorescence analysis of the photosystem II antenna proteins in detergent micelles and liposomes. Biochemistry, 40, 12552–12561.

    CAS  Article  Google Scholar 

  23. Naeem, F., Ahmed, F., Kanwal, M., Murad, W., & Azizullah, A. (2015). Phytotoxicity evaluation of some commonly used shampoos using Brassica napus L. Environmental Science and Pollution Research, 22, 15164–15170. https://doi.org/10.1007/s11356-015-5054-x.

    CAS  Article  Google Scholar 

  24. Naumann, B., Eberius, M., & Appenroth, K. J. (2007). Growth rate based dose–response relationships and EC-values of ten heavy metals using the duckweed growth inhibition test (ISO 20079) with Lemna minor L. clone St. Journal of Plant Physiology, 164, 1656–1664.

    CAS  Article  Google Scholar 

  25. Nyberg, H., & Koskimies-Soininen, K. (1984). The phospholipid fatty acids of Porphyridium purpureum cultured in the presence of triton X-100 and sodium desoxycholate. Phytochemistry, 23, 2489–2493.

    CAS  Article  Google Scholar 

  26. Ogbuagu, D. H., & Iwuchukwu, E. I. (2014). Evaluation of the toxicity of three hair shampoos on the catfish (Clarias gariepinus) fingerlings. Applied Ecology and Environmental Sciences, 2, 86–89.

    Article  Google Scholar 

  27. Okamura, H., Watanabe, T., Aoyama, I., & Hasobe, M. (2002). Toxicity evaluation of new antifouling compounds using suspension-cultured fish cells. Chemosphere, 46, 945–951.

    CAS  Article  Google Scholar 

  28. Ozengin, N., & Elmaci, A. (2007). Performance of duckweed (Lemna minor L.) on different types of wastewater treatment. Journal of Environmental Biology, 28(2), 307–314.

    CAS  Google Scholar 

  29. Pandey, P., & Gopal, B. (2010). Effects of detergents on the growth of two aquatic plants (Azolla pinnata and Hydrilla verticillata). Environment & We: An International Journal of Science and Technology, 5, 107–114.

    Google Scholar 

  30. Prasad, M. N. V., Malec, P., Waloszek, A., Bojko, M., & Strzaka, K. (2001). Physiological responses of Lemna trisulca L. (duckweed) to cadmium and copper bioaccumulation. Plant Science, 161, 881–889.

    CAS  Article  Google Scholar 

  31. Radić, S., Stipaničev, D., Cvjetko, P., Mikelić, I. L., Rajčić, M. M., Širac, S., et al. (2010). Ecotoxicological assessment of industrial effluent using duckweed (Lemna minor L.) as a test organism. Ecotoxicology, 19, 216–222.

    Article  Google Scholar 

  32. Reeder, N. L., Kaplan, J., Xu, J., Youngquist, R. S., Wallace, J., Hu, P., & Saunders, C. W. (2011). Zinc pyrithione inhibits yeast growth through copper influx and inactivation of iron–sulfur proteins. Antimicrobial Agents and Chemotherapy, 55, 5753–5760.

    CAS  Article  Google Scholar 

  33. Richter, E., Wick, A., Ternes, T. A., & Coors, A. (2013). Ecotoxicity of climbazole, a fungicide contained in antidandruff shampoo. Environmental Toxicology and Chemistry, 32, 2816–2825.

    CAS  Article  Google Scholar 

  34. Sánchez-Bayo, F., & Goka, K. (2006). Ecological effects of the insecticide imidacloprid and a pollutant from antidandruff shampoo in experimental rice fields. Environmental Toxicology and Chemistry, 25(6), 1677–1687.

    Article  Google Scholar 

  35. Sanchez-Fortun, S., Marva, F., D’ors, A., & Costas, E. (2008). Inhibition of growth and photosynthesis of selected green microalgae as tools to evaluate toxicity of dodecylethyldimethyl-ammonium bromide. Ecotoxicology, 17, 229–234.

    CAS  Article  Google Scholar 

  36. Shakir, S. K., Kanwal, M., Murad, W., ur Rehman, Z., ur Rehman, S., Daud, M. K., & Azizullah, A. (2016). Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum). Ecotoxicology, 25(2), 329–341.

    CAS  Article  Google Scholar 

  37. Statista (2018). The statistical portal. https://www.statista.com/statistics/276924/us-households-usage-of-shampoo/. Accessed March 14, 2018.

  38. Stebbing, A. R. D. (1982). Hormesis—the stimulation of growth by low levels of inhibitors. Science of the Total Environment, 22, 213–234.

    CAS  Article  Google Scholar 

  39. Turley, P. A., Fenn, R. J., & Ritter, J. C. (2000). Pyrithiones as antifoulants: Environmental chemistry and preliminary risk assessment. Bio-fouling, 15, 175–182.

    CAS  Google Scholar 

  40. Wang, W. (1986). Toxicity tests of aquatic pollutants by using common duckweed. Environmental Pollution, Series B, Chemical and Physical, 11, 1–14.

    CAS  Article  Google Scholar 

  41. Wang, W. (1990). Literature review on duckweed toxicity testing. Environmental Research, 52, 7–22.

    CAS  Article  Google Scholar 

  42. Warne, M. S. J., & Schifko, A. D. (1999). Toxicity of laundry detergent components to a freshwater cladoceran and their contribution to detergent toxicity. Ecotoxicology and Environmental Safety, 44, 196–206.

    CAS  Article  Google Scholar 

  43. Yamane, A. N., Okada, M., & Sudo, R. (1984). The growth inhibition of planktonic algae due to surfactants used in washing agents. Water Research, 18, 1101–1105.

    CAS  Article  Google Scholar 

  44. Yuan, L., Loqué, D., Kojima, S., Rauch, S., Ishiyama, K., et al. (2007). The organization of high affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters. Plant Cell, 19, 2636–2652.

    CAS  Article  Google Scholar 

  45. Zaltauskaite, J., Sujetoviene, G., Cypaite, A., & Auzbikaviciute, A. (2014). Lemna minor as a tool for wastewater toxicity assessment and pollutants removal agent. In Environmental Engineering. Proceedings of the International Conference on Environmental Engineering. ICEE (Vol. 9, p. 1). Vilnius Gediminas Technical University, Department of Construction Economics & Property.

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Acknowledgments

We are thankful to all staff at the Department of Botany, KUST for their support. We also acknowledge KUST for financial support.

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Correspondence to Azizullah Azizullah.

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Azizullah, A., Shakir, S.K., Shoaib, S. et al. Ecotoxicological evaluation of two anti-dandruff hair shampoos using Lemna minor. Environ Monit Assess 190, 268 (2018). https://doi.org/10.1007/s10661-018-6636-0

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Keywords

  • Anti-dandruff
  • Shampoo
  • Ecotoxicology
  • Lemna
  • Aquatic environment