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Seasonal dynamics of freshwater pathogens as measured by microarray at Lake Sapanca, a drinking water source in the north-eastern part of Turkey

  • Reyhan Akçaalan
  • Meric Albay
  • Latife Koker
  • Julia Baudart
  • Delphine Guillebault
  • Sabine Fischer
  • Wilfried Weigel
  • Linda K. MedlinEmail author
Article

Abstract

Monitoring drinking water quality is an important public health issue. Two objectives from the 4 years, six nations, EU Project μAqua were to develop hierarchically specific probes to detect and quantify pathogens in drinking water using a PCR-free microarray platform and to design a standardised water sampling program from different sources in Europe to obtain sufficient material for downstream analysis. Our phylochip contains barcodes (probes) that specifically identify freshwater pathogens that are human health risks in a taxonomic hierarchical fashion such that if species is present, the entire taxonomic hierarchy (genus, family, order, phylum, kingdom) leading to it must also be present, which avoids false positives. Molecular tools are more rapid, accurate and reliable than traditional methods, which means faster mitigation strategies with less harm to humans and the community. We present microarray results for the presence of freshwater pathogens from a Turkish lake used drinking water and inferred cyanobacterial cell equivalents from samples concentrated from 40 into 1 L in 45 min using hollow fibre filters. In two companion studies from the same samples, cyanobacterial toxins were analysed using chemical methods and those dates with highest toxin values also had highest cell equivalents as inferred from this microarray study.

Keywords

Phylochips Microarray Freshwater pathogens Cyanobacteria Molecular barcodes 

Notes

Funding information

This work was supported by the EU μAQUA project (FP7-KBBE-2010-4, 265409).

Supplementary material

10661_2017_6314_MOESM1_ESM.doc (411 kb)
ESM 1 (DOC 411 kb)
10661_2017_6314_MOESM2_ESM.pdf (367 kb)
ESM 2 (PDF 367 kb)

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Reyhan Akçaalan
    • 1
  • Meric Albay
    • 1
  • Latife Koker
    • 1
  • Julia Baudart
    • 2
  • Delphine Guillebault
    • 3
  • Sabine Fischer
    • 4
  • Wilfried Weigel
    • 4
  • Linda K. Medlin
    • 5
    Email author
  1. 1.Faculty of Aquatic SciencesIstanbul UniversityIstanbulTurkey
  2. 2.Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire OcéanologiqueBanyuls/MerFrance
  3. 3.Microbia EnvironnementBanyuls sur MerFrance
  4. 4.Scienion AG VolmerstrBerlinGermany
  5. 5.Marine Biological Association of the UKPlymouthUK

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