The goal of the present study is to assess the impact of the experimental conditions for extraction procedures (time of extraction, thermal treatment and type of extraction media) as applied to several baby and infant products checked for their possible ecotoxicological response when tested by various ecotoxicity tests (Microtox®, Ostracodtoxkit F™ and Xenoscreen YES/YAS™). The systems under consideration are multidimensional by nature and, therefore, the appropriate assessment approach was intelligent data analysis (chemometrics). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were selected as reliable data mining methods for the interpretation of the ecotoxicity data. We show that the different experimental conditions have a significant impact on the ecotoxicity levels observed, especially those measured by Microtox® and Ostracodtoxkit F™ tests. The time of contact proves to be a very significant factor for all extraction media and ecotoxicity test procedures. The present study is a pioneering effort to offer a specific expert approach for analysing links between the type of test measurement methodology and imposed experimental conditions to mimic real-life circumstances in the use of baby and infant products.
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Chang, S. C., Wang, Y. F., You, S. J., Kuo, Y. M., Tsai, C. H., Wang, L. C., & Hsu, P. Y. (2012). Toxicity evaluation of fly ash by Microtox®. Areosol Air Quality Research, 13, 1002–1008.
Czech, B., Jośko, I., & Oleszczuk, P. (2014). Ecotoxicological evaluation of selected pharmaceuticals to Vibrio fischeri and Daphnia magna before and after photooxidation process. Ecotoxicology and Environmental Safety, 104, 247–253.
Deljanin, I., Antanasijević, D., Bjelajac, A., Urošević, M. A., Nikolić, M., Perić-Grujić, A., & Ristić, M. (2016). Chemometrics in biomonitoring: distribution and correlation of trace elements in tree leaves. Science of the Total Environment, 545–546, 361–371.
DIN 53160-1:2010-10 (2010). Determination of the colourfastness of articles for common use - Part 1: Test with artificial saliva. https://www.beuth.de/en/standard/din-53160-1/133532232. Accessed 23 May 2017.
DIN 53160-2:2010-10 (2010). Determination of the colourfastness of articles for common use - Part 1: Test with artificial sweat. https://www.beuth.de/en/standard/din-53160-2/133532317. Accessed 23 May 2017.
Dubiella-Jackowska, A., Astel, A., Polkowska, Ż., Staszek, W., Kudłak, B., & Namieśnik, J. (2010). Atmospheric and surface water pollution interpretation in the Gdańsk Beltway impact range by the use of multivariate analysis. Clean – Soil, Air, Water, 38, 865–876.
Faa, G., Ekstrom, J., Castagnola, M., Gibo, Y., Ottonello, G., & Fanos, V. (2012). A developmental approach to drug-induced liver injury in newborns and children. Current Medicinal Chemistry, 19, 4581–5491.
Guney, M., & Zagury, G. J. (2014). Children’s exposure to harmful elements in toys and low-cost jewelry: characterizing risks and developing a comprehensive approach. Journal of Hazardous Materials, 271, 321–330.
Hernández-Fernández, F. J., Bayo, J., Pérez de los Ríos, A., Vicente, M. A., Bernal, F. J., & Quesada-Medina, J. (2015). Discovering less toxic ionic liquids by using the Microtox® toxicity test. Ecotoxicology and Environmental Safety, 116, 29–33.
Ionas, A. C., Ulevicus, J., Gómez, A. B., Brandsma, S. H., Leonards, P. E. G., van de Bor, M., & Covaci, A. (2016). Children’s exposure to polybrominated diphenyl ethers (PBDEs) through mouthing toys. Environment International, 87, 101–107.
Korfali, S. J., Sebra, R., Jurdi, M., & Taleb, R. J. (2013). Assessment of toxic metals and phthalates in children’s toys and clays. Archives of Environmental Contamination and Toxicology, 65, 368–381.
Kudłak, B., Wolska, L., & Namieśnik, J. (2011). Determination of EC50 toxicity data of selected heavy metals toward Heterocypris incongruens. Environmental Monitoring and Assessment, 174, 509–516.
Kudłak, B., Owczarek, K., & Namieśnik, J. (2015a). A review of selected issues related to the toxicity of ionic liquids and deep eutectic solvents. Environmental Science and Pollution Research, 22, 11975–11992.
Kudłak, B., Szczepańska, N., Owczarek, K., Mazerska, Z., & Namieśnik, J. (2015b). Revision of biological methods for determination of EDC presence and their endocrine potential. Critical Reviews in Analytical Chemistry, 45, 191–200.
Li, X., Ying, G. G., Su, H. C., Yang, H. B., & Wang, L. (2010). Simultaneous determination and assessment of 4-nonylphenol, bisphenol A and triclosan in tap water, bottled water and baby bottlers. Environment International, 36, 557–562.
López-Doval, J. C., Meirelles, S. T., Cardoso-Silva, S., Moschini-Carlos, V., & Pompêo, M. (2016). Ecological and toxicological responses in a multistressor scenario: are monitoring programs showing the stressors or just showing stress? A case study in Brazil. Science of the Total Environment, 540, 466–476.
Massart, D. L., & Kaufmann, L. (1983). The analytical data interpretation by the use of cluster analysis. Amsterdam: Elsevier.
Mercan, S., Ellez, S. Z., Türkmen, Z., Yayla, M., & Cengiz, S. (2015). Quantitative lead determination in coating paint on children’s outwear by LA-ICP-MS: a practical calibration strategy for solid samples. Talanta, 132, 222–227.
Özer, E. T., & Güçer, S. (2012). Determination of di(2-ethylhexyl) phthalate migration from toys into artificial sweat by gas chromatography mass spectrometry after activated carbon enrichment. Polymer Testing, 31, 474–480.
Peré-Trepat, E., Olivella, L., Ginebreda, A., Caixach, J., & Tauler, R. (2006). Chemometrics modelling of organic contaminants in fish and sediment river samples. Science of the Total Environment, 371, 223–237.
Platikanov, S., Martín, J., & Tauler, R. (2012). Linear and non-linear chemometric modeling of THM formation in Barcelona’s water treatment plant. Science of the Total Environment, 432, 365–374.
Rossetto, A. L., Melegari, S. P., Ouriques, L. C., & Matias, W. G. (2014). Comparative evaluation of acute and chronic toxicities of CuO nanoparticles and bulk using Daphnia magna and Vibrio fischeri. Science of the Total Environment, 490, 807–814.
Szczepańska, N., Namieśnik, J., & Kudłak, B. (2016). Assessment of toxic and endocrine potential of substances migrating from selected toys and baby products. Environmental Science and Pollution Research, 23, 24890–24900.
Thomas, K. V., Langford, K., Petersen, K., Smith, A. J., & Tollefsen, K. E. (2009). Effect-directed identification of naphthenic acids as important in vitro xeno-estrogens and anti-androgens in North Sea offshore produced water discharges. Environmental Science and Technology, 43, 8066–8071.
Vanderginste, B., Massart, D. L., Buydens, L., De Jong, S., Lewi, P., & Smeyers-Verbeke, J. (1998). Handbook of chemometrics and qualimetrics. Amsterdam: Elsevier.
Ventura, S. P. M., Marques, C. S., Rosatella, A. A., Afonso, C. A. M., Gonc-alves, F., & Coutinho, J. A. P. (2012). Toxicity assessment of various ionic liquid families towards Vibrio fischeri marine bacteria. Ecotoxicology and Environmental Safety, 76, 162–168.
Wieczerzak, M., Kudłak, B., & Namieśnik, J. (2016). Bioassays as one of the green chemistry tools for assessing environmental quality: a review. Environment International, 94, 341–361.
This work has been co-financed by the Science National Centre, Poland grant no. 2015/17/N/ST4/03835.
The support of H2020 programme of the European Union (project Materials Networking) is gratefully acknowledged by two of the authors (M. Nedyalkova and V. Simeonov).
• Artificial sweat and saliva were used to prepare samples for toxicological studies.
• Microtox® toxicity is reversely correlated with endocrine potential values.
• The antagonistic endocrine potential of products for infants was confirmed.
• Multivariate statistical techniques were used to analyse such elaborate dynamic systems.
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Szczepańska, N., Kudłak, B., Nedyalkova, M. et al. Application of chemometric techniques in studies of toxicity of selected commercially available products for infants and children. Environ Monit Assess 189, 309 (2017). https://doi.org/10.1007/s10661-017-6007-2
- Multivariate statistics
- Endocrine potential
- Artificial human fluids
- Products for infants