Skip to main content

Advertisement

SpringerLink
Log in

Environmental monitoring and assessment of antibacterial metabolite producing actinobacteria screened from marine sediments in south coastal regions of Karnataka, India

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Assessment of the therapeutic potential of secondary metabolite producing microorganisms from the marine coastal areas imparts scope and application in the field of environmental monitoring. The present study aims to screen metabolites with antibacterial potential from actionbacteria associated with marine sediments collected from south coastal regions of Karnataka, India. The actinobacteria were isolated and characterized from marine sediments by standard protocol. The metabolites were extracted, and antibacterial potential was analyzed against eight hospital associated bacteria. The selected metabolites were partially characterized by proximate analysis, SDS-PAGE, and FTIR-spectroscopy. The antibiogram of the test clinical isolates revealed that they were emerged as multidrug-resistant strains (P ≤ 0.05). Among six actinobacteria (IS1-1S6) screened, 100 μl−1 metabolite from IS1 showed significant antibacterial activities against all the clinical isolates except Pseudomonas aeruginosa. IS2 demonstrated antimicrobial potential towards Proteus mirabilis, Streptococcus pyogenes, and Escherichia coli. The metabolite from IS3 showed activity against Strep. pyogenes and E. coli. The metabolites from IS4, IS5, and IS6 exhibited antimicrobial activities against Ps. aeruginosa (P ≤ 0.05). The two metabolites that depicted highest antibacterial activities against the test strains were suggested to be antimicrobial peptides with low molecular weight. These isolates were characterized and designated as Streptomyces sp. strain mangaluru01 and Streptomyces sp. mangaloreK01 by 16S ribosomal DNA (rDNA) sequencing. This study suggests that south coastal regions of Karnataka, India, are one of the richest sources of antibacterial metabolites producing actinobacteria and monitoring of these regions for therapeutic intervention plays profound role in healthcare management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abd-Elnaby, H., Abo-Elala, G., Abdel-Raouf, U., Abd-elwahab, A., & Hamed, M. (2016). Antibacterial and anticancer activity of marine Streptomyces parvus; optimization and application. Biotechnology & Biotechnological Equipment, 30, 180–191.

    Article  CAS  Google Scholar 

  • Aryantha, N. P., Adinda, A., & Kusmaningat, S. (2002). Occurrence of triterpenoids and polysaccharides on Ganoderma tropicum with Ganoderma lucidum as reference. Austin Mycology, 20, 123–129.

    Google Scholar 

  • Bauer, A. W., Perry, D. M., & Kirby, W. M. M. (1959). Single disc antibiotic sensitivity testing of Staphylococci. An analysis of technique and results. AMA Archives of Internal Medicine, 104, 208–216.

    CAS  Google Scholar 

  • Clinical and Laboratory Standards Institute (2015). M100-S25. Performance standards for antimicrobial susceptibility testing: 25th informational supplement. Clinical and Laboratory Standards Institute, Wayne.

  • Firdouse, S., & Alam, P. (2011). Phytochemical investigation of extract of Amorphophallus campanulatus tubers. International Journal of Phytomedicine, 3, 32–35.

    Google Scholar 

  • Ghanem, N. B., Sabry, S. A., El-Sherif, Z. M., & Abu El-Ela, G. A. (2000). Isolation and enumeration of marine actinomycetes from seawater and sediments in Alexandria. Journal of General & Applied Microbiology, 46, 105–111.

    Article  CAS  Google Scholar 

  • Golinska, P., Wypij, M., Agarkar, G., Rathod, D., Dahm, H., & Rai, M. (2015). Endophytic actinobacteria of medicinal plants: diversity and bioactivity. Antonie Van Leeuwenhoek, 108, 267–289.

    Article  Google Scholar 

  • Guo, X., Liu, N., Li, X., Ding, Y., Shang, F., Gao, Y., Ruan, J., & Huang, Y. (2015). Red soils harbor diverse culturable actinomycetes that are promising sources of novel secondary metabolites. Applied and Environmental Microbiology, 81, 3086–3103.

    Article  CAS  Google Scholar 

  • Hamedi, J., Imanparast, S., & Mohammadipanah, F. (2015). Molecular, chemical and biological screening of soil actinomycete isolates in seeking bioactive peptide metabolites. Iranian Journal of Microbiology, 7, 23–30.

    Google Scholar 

  • Jeyanthi, V., & Velusamy, P. (2016). Anti-methicillin resistant Staphylococcus aureus compound isolation from halophilic Bacillus amyloliquefaciens MHB1 and determination of its mode of action using electron microscope and flow cytometry analysis. Indian Journal of Microbiology, 56, 148–157.

    Article  CAS  Google Scholar 

  • Kruskala, W. H., & Wallisa, W. A. (1952). Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association, 47, 583–621.

    Article  Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  • Mohseni, M., Norouzi, H., Hamedi, J., & Roohi, A. (2013). Screening of antibacterial producing actinomycetes from sediments of the Caspian sea. International Journal of Molecular and Cellular Medicine, 2, 64–71.

    Google Scholar 

  • Murray, P. R., Baron, J. H., Pfaller, M. A., Jorgensen, J. H., Yolken, R. H. (2003). Manual of clinical microbiology, 8th Ed. American Society for Microbiology, Washington D.C.

  • Rajan, B. M., & Kannabiran, K. (2014). Extraction and identification of antibacterial secondary metabolites from marine Streptomyces sp. VITBRK2. International Journal of Molecular and Cellular Medicine, 3, 130–137.

    CAS  Google Scholar 

  • Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.

    CAS  Google Scholar 

  • Silverstein, R. M., Bassler, G. C., & Morrill, T. C. (1981). Spectrometric identification of organic compounds (4th ed.). New York: John Wiley and Sons.

    Google Scholar 

  • Singh, D., Singh, P., Gupta, A., Solanki, S., Sharma, E., & Nema, R. (2012). Qualitative estimation of the presence of bioactive compound in Centella asiatica: an important medicinal plant. International Journal of Life Sciences and Medical Research, 2, 5–7.

    Article  Google Scholar 

  • Subramani, R., & Aalsberg, W. (2012). Marine actinomycetes: an ongoing source of novel bioactive metabolites. Microbiological Research, 167, 571–580.

    Article  CAS  Google Scholar 

  • Suthindhiran, K., & Kannibiran, K. (2010). Diversity and exploration of bioactive marine actinomycetes in the Bay of Bengal of Puducherry coast of India. Indian Journal of Microbiology, 50, 76–82.

    Article  CAS  Google Scholar 

  • Tanwar, J., Das, S., Fatima, S., & Hameed, S. (2014). Multidrug resistance: an emerging crisis. Interdisciplinary Perspectives on Infectious Diseases, 2014, 541340. doi:10.1155/2014/541340.

    Article  Google Scholar 

  • Trabelsi, I., Oves, D., Manteca, A., Genilloud, O., Altalhi, A., & Nour, M. (2016). Antimicrobial activities of some actinomycetes isolated from different rhizospheric soils in Tunisia. Current Microbiology, 73, 220–227.

    Article  CAS  Google Scholar 

  • Venter, H., Henningsen, M. L., & Begg, S. L. (2017). Antimicrobial resistance in healthcare, agriculture and the environment: the biochemistry behind the headlines. Essays in Biochemistry, 61, 1–10. doi:10.1042/EBC20160053.

    Article  Google Scholar 

  • Zainal, N., Ser, H. L., Yin, W. F., Tee, K. K., Lee, L. H., & Chan, K. G. (2016). Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest. Antonie Van Leeuwenhoek, 109, 467–474.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. R & D Centre, Department of Biotechnology, Dayananda Sagar College of Engineering, Bangalore, Karnataka, 560078, India

    Sinosh Skariyachan, Shruthi Garka, Sushmitha Puttaswamy, Shobitha Shanbhogue, Raksha Devaraju & Rajeswari Narayanappa

Authors
  1. Sinosh Skariyachan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shruthi Garka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sushmitha Puttaswamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shobitha Shanbhogue
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Raksha Devaraju
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rajeswari Narayanappa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sinosh Skariyachan.

Electronic supplementary material

Table S1

(DOC 32 kb)

Table S2

(DOC 32 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Skariyachan, S., Garka, S., Puttaswamy, S. et al. Environmental monitoring and assessment of antibacterial metabolite producing actinobacteria screened from marine sediments in south coastal regions of Karnataka, India. Environ Monit Assess 189, 283 (2017). https://doi.org/10.1007/s10661-017-5999-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10661-017-5999-y

Keywords

Search

Navigation