Antibiotic-resistant E. coli in surface water and groundwater in dairy operations in Northern California
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Generic Escherichia coli was isolated from surface water and groundwater samples from two dairies in Northern California and tested for susceptibility to antibiotics. Surface samples were collected from flush water, lagoon water, and manure solids, and groundwater samples were collected from monitoring wells. Although E. coli was ubiquitous in surface samples with concentrations ranging from several hundred thousand to over a million colony-forming units per 100 mL of surface water or per gram of surface solids, groundwater under the influence of these high surface microbial loadings had substantially fewer bacteria (3- to 7-log10 reduction). Among 80 isolates of E. coli tested, 34 (42.5 %) were resistant to one or more antibiotics and 22 (27.5 %) were multi-antibiotic resistant (resistant to ≥3 antibiotics), with resistance to tetracycline, cefoxitin, amoxicillin/clavulanic acid, and ampicillin being the most common. E. coli isolates from the calf hutch area exhibited the highest levels of multi-antibiotic resistance, much higher than isolates in surface soil solids from heifer and cow pens, flush alleys, manure storage lagoons, and irrigated fields. Among E. coli isolates from four groundwater samples, only one sample exhibited resistance to ceftriaxone, chloramphenicol, and tetracycline, indicating the potential of groundwater contamination with antibiotic-resistant bacteria from dairy operations.
KeywordsDairy Water E. coli Antibiotic resistance
The authors are grateful to Kathy Glenn, Veterinary Medicine Teaching and Research Center, University of California, Davis, for the technical assistance with the MIC method. The authors thank Drs. Bruce Hoar and Christine Kreuder Johnson, School of Veterinary Medicine, University of California, Davis, for the consultations on regulations of antibiotics in dairies. We gratefully acknowledge funding for this work by the California State Water Resources Control Board under agreement no. 04-184-555-3.
- Chee-Sanford, J. C., Aminov, R. I., Krapac, I. J., Garrigues-Jeanjean, N., & Mackie, R. I. (2001). Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities. Applied and Environmental Microbiology, 67(4), 1494–1502.CrossRefGoogle Scholar
- Dimri, G. P., Rudd, K. E., Morgan, M. K., Bayat, H., & Ames, G. F. (1992). Physical mapping of repetitive extragenic palindromic sequences in Escherichia coli and phylogenetic distribution among Escherichia coli strains and other enteric bacteria. Journal of Bacteriology, 174(14), 4583–4593.Google Scholar
- Dodson, K., & LeJeune, J. (2005). Escherichia coli O157:H7, Campylobacter jejuni, and Salmonella prevalence in cull dairy cows marketed in northeastern Ohio. Journal of Food Protection, 68(5), 927–931.Google Scholar
- Hoar, B. R., Atwill, E. R., Elmi, C., Utterbach, W. W., & Edmondson, A. (1999). Comparison of fecal samples collected per rectum and off the ground for estimation of environmental contamination attributable to beef cattle. American Journal of Veterinary Research, 60(11), 1352–1356.Google Scholar
- Kenny, J., Barber, N., Hutson, S., Linsey, K., Lovelace, J., & Maupin, M. (2009). Estimated use of water in the United States in 2005. U.S. Geological Survey Circular 1344. 52 p.Google Scholar
- Koike, S., Krapac, I. G., Oliver, H. D., Yannarell, A. C., Chee-Sanford, J. C., Aminov, R. I., et al. (2007). Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period. Applied and Environmental Microbiology, 73(15), 4813–4823.CrossRefGoogle Scholar
- Morris, B. L., Lawrence, A. R. L., Chilton, P. J. C., Adams, B., Calow, R. C., & Klinck, B. A. (2003). Groundwater and its susceptibility to degradation: A global assessment of the problem and options for management (Early Warning and Assessment Report Series, RS. 03-3). Nairobi: United Nations Environment Programme.Google Scholar
- Pradhan, A. K., Van Kessel, J. S., Karns, J. S., Wolfgang, D. R., Hovingh, E., Nelen, K. A., et al. (2009). Dynamics of endemic infectious diseases of animal and human importance on three dairy herds in the northeastern United States. Journal of Dairy Science, 92(4), 1811–1825.CrossRefGoogle Scholar
- Shere, J. A., Bartlett, K. J., & Kaspar, C. W. (1998). Longitudinal study of Escherichia coli O157:H7 dissemination on four dairy farms in Wisconsin. Applied and Environmental Microbiology, 64(4), 1390–1399.Google Scholar
- Stanton, A. L., Kelton, D. F., Leblanc, S. J., Millman, S. T., Wormuth, J., Dingwell, R. T., & Leslie, K. E. (2010). The effect of treatment with long-acting antibiotic at postweaning movement on respiratory disease and on growth in commercial dairy calves. Journal of Dairy Science, 93(2), 574–581.Google Scholar
- Stanton, A. L., Kelton, D. F., LeBlanc, S. J., Wormuth, J., & Leslie, K. E. (2012). The effect of respiratory disease and a preventative antibiotic treatment on growth, survival, age at first calving, and milk production of dairy heifers. Journal of Dairy Science, 95(9), 4950–4960.CrossRefGoogle Scholar
- USDA APHIS Antibiotic Use on U.S. Dairy Operations, 2002 and 2007. Retrieved from http://www.aphis.usda.gov/animal_health/nahms/dairy/downloads/dairy07/Dairy07_is_AntibioticUse.pdf.
- Watanabe, N., Harter, T., & Bergamaschi, B. A. (2008). Environmental occurrence and shallow ground water detection of the antibiotic monensin from dairy farms. Journal of Environmental Quality, 37(5), S78–S85.Google Scholar