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Habitat monitoring and genotoxicity in Ucides cordatus (Crustacea: Ucididae), as tools to manage a mangrove reserve in southeastern Brazil

Environmental Monitoring and Assessment volume 185, pages 8273–8285 (2013)Cite this article

Abstract

In Brazil, the state of São Paulo contains both preserved areas (Juréia-Itatins Ecological Station) and extremely impacted ones (Cubatão Municipality). This study evaluated the concentrations of five metals (Cu, Cd, Cr, Pb, and Hg) in two mangroves with different levels of anthropogenic impact and the apparent genotoxicity to Ucides cordatus. Water and sediment samples were obtained, and metal concentrations were determined with an atomic absorption spectrophotometer. The genotoxic impact was quantified based on the number of micronucleated cells per 1,000 analyzed (MN‰), using hemolymph slides stained with Giemsa. Metal concentrations in water were below the detection limit, except for lead, although no significant difference was observed between the areas (P > 0.05). Sediment from Cubatão had higher concentrations of Cd, Pb, Cr, and Cu than sediment from Juréia-Itatins (P < 0.05), but no significant differences in metal concentrations were detected among depth strata of the sediment (P > 0.05). Crabs from Cubatão had a 2.6 times higher mean frequency of micronucleated cells (5.2 ± 1.8 MN‰) than those from Juréia-Itatins (2.0 ± 1.0 MN‰; P < 0.0001). The more-polluted conditions found in the mangrove sediments of Cubatão were reflected in the micronucleus assay, demonstrating their genotoxic effect; however, genetic damage should be attributed to a synergistic effect with other kinds of pollutants previously recorded in different environments of Cubatão. U. cordatus proved to be an excellent bioindicator of mangrove pollution. This study established, for the first time, the normal frequency of MN‰ in a population of this species within an ecological station.

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Acknowledgments

To the “Fundação de Amparo à Pesquisa do Estado de São Paulo” (FAPESP) for awarding financial aid to MAAP during Project Uçá II and III (# 2002/05614-2 and # 2009/14725-1, respectively) and an undergraduate scholarship to TRT (# 2006/05976-2); to biologist Karina R. S. Banci and Mr. Dorico R. Lima, for their help during field expeditions in the mangroves; and to Dr. Janet Reid (JWR Associates) who revised the English. MAAP and RAT are grateful to CNPq for the research fellowships provided.

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Authors and Affiliations

  1. Laboratório de Biologia de Crustáceos-Praça Infante D. Henrique, UNESP-Univ Estadual Paulista, Campus Experimental do Litoral Paulista (CLP), s/n, Parque Bitaru, 11330-900, São Vicente, São Paulo, Brazil

    M. A. A. Pinheiro, L. F. A. Duarte & T. R. Toledo

  2. Centro Acadêmico de Vitória-Rua do Alto do Reservatório, UFPE-Universidade Federal de Pernambuco, s/n, Bela Vista, 55608-680, Vitória de Santo Antão, Pernambuco, Brazil

    M. L. Adam

  3. Centro de Ciências Biológicas, Departamento de Zoologia, Laboratório de Genômica Evolutiva e Ambiental-Rua Prof. Nelson Chaves, UFPE-Universidade Federal de Pernambuco, s/n, Cidade Universitária, Recife, Pernambuco, Brazil

    R. A. Torres

  4. Research Group on Crustacean Biology (CRUSTA), São Paulo, Brazil

    M. A. A. Pinheiro, L. F. A. Duarte, T. R. Toledo, M. L. Adam & R. A. Torres

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  1. M. A. A. Pinheiro
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  2. L. F. A. Duarte
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  3. T. R. Toledo
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  4. M. L. Adam
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Correspondence to M. A. A. Pinheiro.

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Pinheiro, M.A.A., Duarte, L.F.A., Toledo, T.R. et al. Habitat monitoring and genotoxicity in Ucides cordatus (Crustacea: Ucididae), as tools to manage a mangrove reserve in southeastern Brazil. Environ Monit Assess 185, 8273–8285 (2013). https://doi.org/10.1007/s10661-013-3172-9

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  • DOI: https://doi.org/10.1007/s10661-013-3172-9

Keywords

  • Contamination
  • Ecological station
  • Estuary
  • Mangrove
  • Metal
  • Micronucleus