Use of three bivalve species for biomonitoring a polluted estuarine environment
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Estuaries are marine areas at great contamination risk due to their hydrodynamic features. PAH are wide and ubiquitous contaminants with a high presence in these marine environments. Chemical analysis of sediments can provide information, although it does not give a direct measure of the toxicological effect of such contaminants in the biota. Samples of Venerupis pullastra, Cerastoderma edule, and Mytilus galloprovincialis were collected from two locations in Corcubión estuary (Norhwest of Spain). The level of PAH in sediment and biota, and its possible origin were assessed. A moderate level of contamination was observed with a predominance of PAH of a pyrogenic origin. Genotoxic damage, measured as single-strand DNA breaks with the comet assay, was evaluated in gill tissue and in hemolymph. The values of DNA damage obtained showed a higher sensitivity of clams and cockles to the pollution load level. These differences among species make us suggest the use of some other species coupled with mussels as an optimal tool for biomonitoring estuarine environments.
KeywordsEstuary Comet assay Bivalve Polycyclic aromatic hydrocarbons Marine pollution
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- Baumard, P., Budzinski, H., Garrigues, P., Narbone, J. F., Burgeot, T., Michel, X., et al. (1999). Polycylic Aromatic Hydrocarbon (PAH) burden of mussels (Mytilus sp.) in different marine environments in relation with sediment PAH contamination, and bioavailability. Marine Environmental Research, 47, 415–439.CrossRefGoogle Scholar
- Bernstein, C., & Bernstein, H. (1991). Ageing, sex and DNA repair. New York: Academic Press.Google Scholar
- Chase, M. E., Jones, S. H., Hennigar, P., Sowles, J., Harding, G. C. H., Freeman, K., et al. (2001). Gulfwatch: Monitoring spatial and temporal patterns of trace metal and organic contaminants in the gulf of Maine (1991–1997) with the blue mussel, Mytilus edulis L. Marine Pollution Bulletin, 42, 490–504.CrossRefGoogle Scholar
- Colombo, J., Cappelletti, N., Lasci, J., Migoya, M., Speranza, E., & Skorupka, C. (2006). Sources, vertical fluxes, and equivalent toxicity of aromatic hydrocarbons in coastal sediments of the río de la Plata estuary, Argentina. Environental and Science Technology, 40, 734–740.CrossRefGoogle Scholar
- Coughlan, B. M., Hartl, M. G. J., O’Reilly, S. J., Sheehan, D., Morthersill, C., van Pelt, F., et al. (2002). Detecting genotoxicity using the comet assay following chronic exposure of manila clam Tapes semidecussatus to polluted estuarine sediments. Marine Pollution Bulletin, 44, 1359–1365.CrossRefGoogle Scholar
- Downs, C. A., Shigenaka, G., Fauth, J. E., Robinson, C. E., & Huang, A. (2002). Cellular physiological assessment of bivalves after chronic exposure to spilled Exxon Valdez crude oil using a novel molecular diagnostic biotechnology. Environmental Science and Technology, 36, 2987–2993.CrossRefGoogle Scholar
- Farmer, P. B. (2003). Molecular epidemiology studies of carcinogenic environmental pollutants. Effects of Polycyclic Aromatic Hydrocarbons (PAHs) in environmental pollution on exogenous and oxidative DNA damages. Mutation Research-Reviews in Mutation Research, 544, 397–402.Google Scholar
- Khadim, M. (1990). Methodologies for monitoring the genetic effects of mutagens and carcinogens accumulated in the body of marine mussels. Reviews in Aquatic Science, 2, 83–107.Google Scholar
- Large, A. T., Shaw, J. P., Peters, L. D., McIntosh, A. D., Webster, L., Mally, A., et al. (2002). Different levels of mussel (Mytilus edulis) DNA strand breaks following chronic field and acute laboratory exposure to polycyclic aromatic hydrocarbons. Marine Environmental Research, 54, 493–497.CrossRefGoogle Scholar
- Lemiere, S., Cossu-Leguille, C., Bispo, A., Jourdain, M.-J., Lanhers, M.-C., Burnel, D., et al. (2005). DNA damage measured by the single-cell electrophoresis (Comet) assay in mammals fed with mussels contaminated by the “Erika” oil-spill. Mutation Research, 581, 11–21.Google Scholar
- Livingstone, D. R. (1993). Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. Journal of Chemical Technology and Biotechnology, 57, 195–211.Google Scholar
- Monserrat, J. M., Martínez, P. E., Geracitano, L. A., Lund Amado, L., Martinez Gaspar Martins, C., Lopes Leães Pinho, G., et al. (2007). Pollution biomarkers in estuarine animals: Critical review and new perspectives. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 146, 221–34.CrossRefGoogle Scholar
- Neff, J. (1979). Polycyclic aromatic hydrocarbons in the aquatic environment: Sources, fates and biological effects. Essex: Applied Science Publishers Ltd.Google Scholar
- Pérez-Cadahía, B., Laffon, B., Pásaro, E., & Méndez, J. (2004). Evaluation of PAH bioaccumulation and DNA damage in mussels (Mytilus galloprovincialis) exposed to spilled prestige crude oil. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 138, 453–460.CrossRefGoogle Scholar
- Serafim, M. A., & Bebianno, M. J. (2001). Variation of metallothionein and metal concentrations in the digestive gland of the clam Ruditapes decussatus: Sex and seasonal effects. Environmental Toxicology and Chemistry, 20, 544–552.Google Scholar
- Thomas, R. E., Lindeberg, M., Harris, P. M., & Rice, S. D. (2007). Induction of DNA strand breaks in the mussel (Mytilus trossulus) and clam (Protothaca staminea) following chronic field exposure to polycyclic aromatic hydrocarbons from the Exxon Valdez spill. Marine Pollution Bulletin, 54, 726–732.CrossRefGoogle Scholar
- Vlahogianni, T., Dassenakis, M., Scoullos, M. J., & Valavanidis, A. (2007). Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals’ pollution in coastal areas from the Saronikos gulf of Greece. Marine Pollution Bulletin, 54, 1361–1371.CrossRefGoogle Scholar
- Wessel, N., Santos, R., Menard, D., Le Menach, K., Buchet, V., Lebayon, N., et al. (2010). Relationship between PAH biotransformation as measured by biliary metabolites and EROD activity, and genotoxicity in juveniles of sole (Solea solea). Marine Environmental Research. doi:10.1016/j.marenvres.2010.03.004.