Skip to main content

Advertisement

Log in

Determining subsampling effort for the development of a rapid bioassessment protocol using benthic macroinvertebrates in streams of Southeastern Brazil

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

The purpose of this study was to establish a subsampling procedure for benthic macroinvertebrates to aid in the development of a multimetric index to assess the biological condition of streams. Data from six streams that are considered minimally impaired were used. Subsampling was done using a device divided into 24 quadrats. The sediment from each quadrat was sorted, and all organisms were removed and identified. Richness metrics were the most affected by subsample size. Relative-abundance metrics were the most stable, proving that the sample was well distributed throughout the tray and abundance proportions were maintained. The results showed that the macroinvertebrate assemblage present in the six quadrats was similar to that present in the full sample. These analyses indicated that six quadrats, randomly designated, with a minimum of 200 collected specimens could be used in place of the full sample. In routine water management, managing time and costs are a major challenge; therefore, this type of simplification is absolutely necessary to ensure that a biomonitoring tool is useful for practical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • AQEM Consortium (2002). Manual for the application of the AQEM system. A comprehensive method to assess European streams using benthic macroinvertebrates, developed for the purpose of the Water Framework Directive.

  • Araújo, F. G., Fichberg, I., Pinto, B. C. T., & Peixoto, M. G. (2003). A preliminary index of biotic integrity for monitoring the condition of the Rio Paraíba do Sul, southeast Brazil. Environmental Management, 32, 516–526.

    Article  Google Scholar 

  • Baptista, D. F., Buss, D. F., Egler, M., Giovanelli, A., Silveira, M. P., & Nessimian, J. (2007). A multimetric index based on benthic macroinvertebrates for evaluation of Atlantic Forest streams at Rio de Janeiro State, Brazil. Hydrobiologia, 575, 83–94.

    Article  Google Scholar 

  • Barbour, M. T., & Gerritsen, J. (1996). Subsampling of benthic samples: A defense of the fixed-count method. Journal of the North American Benthological Society, 15, 386–391.

    Article  Google Scholar 

  • Barbour, M. T., Gerritsen, J., Snyder, B. D., & Stribling, J. B. (1999). Rapid bioassessment protocols for use in streams and wadeable rivers: Periphyton, benthic macroinvertebrates and fish (2nd Ed., p. 339). Washington, DC: EPA 841-B-99-002, Office of Water, US Environmental Protection Agency.

    Google Scholar 

  • Bozzeti, M., & Schulz, U. H. (2004). An index of biotic integrity based on fish assemblages for subtropical streams in southern Brazil. Hydrobiologia, 529, 133–144.

    Article  Google Scholar 

  • Buss, D. F., & Borges, E. L. (2008). Aplication of Rapid Bioassessment Protocols (RBP) for benthic macroinvertebrates in Brazil: Comparison between sampling techniques and mesh sizes. Neotropical Entomology, 37, 288–295.

    Article  Google Scholar 

  • Cao, Y., Williams, D. D., & Williams, N. E. (1998). How important are rare species in aquatic community ecology and bioassessment? Limnology and Oceanography, 43, 1403–1409.

    Article  Google Scholar 

  • Clarke, R. T., Lorenz, A., Sandin, L., Schmidt-Kloiber, A., Strackbein, J., Kneebone, N. T., et al. (2006). Effects of sampling and sub-sampling variation using the STAR-AQEM sampling protocol on the precision of macroinvertebrate metrics. Hydrobiologia, 566, 451–459.

    Google Scholar 

  • Coutermanch, D. L. (1996). Commentary on the subsampling procedures used for rapid bioassessments. Journal of the North American Benthological Society, 15, 381–385.

    Article  Google Scholar 

  • Doberstein, C. P., Karr, J. R., & Conquest, L. L. (2000). The effect of fixed-count subsampling on macroinvertebrate biomonitoring in small streams. Freshwater Biology, 44, 355–371.

    Article  Google Scholar 

  • Haase, P., Lohse, S., Pauls, S., Schindehütte, K., Sundermann, A., Rolauffs, P., et al. (2004). Assessing streams in Germany with benthic invertebrates: Development of a practical standardised protocol for macroinvertebrate sampling and sorting. Limnologica, 34, 349–365.

    Article  Google Scholar 

  • Hering, D., Moog, O., Sandin, L., & Verdonschot, P. F. M. (2004). Overview and application of the AQEM assessment system. Hydrobiologia, 516, 1–20.

    Article  Google Scholar 

  • Karr, J. R. (1991). Biological integrity: A long-neglected aspect of water resource management. Ecological Applications, 1, 66–84.

    Article  Google Scholar 

  • Karr, J. R. (1998). Rivers as sentinels: Using the biology of rivers to guide landscape management. The ecology and management of streams and rivers in the Pacific Northwest Coastal Ecoregion (pp. 502–528). New York: Springer.

    Google Scholar 

  • Karr, J. R., & Chu, E. W. (1999). Restoring life in running waters: Better biological monitoring (p. 206). Washington, DC: Island Press.

    Google Scholar 

  • King, R. S., & Richardson, C. J. (2002). Evaluating subsampling approaches and macroinvertebrate taxonomic resolution for wetland bioassessment. Journal of the North American Benthological Society, 21, 150–171.

    Article  Google Scholar 

  • Krebs, C. J. (1998). Ecological methodology (2nd Ed.). Menlo Park: Addison-Wesley.

    Google Scholar 

  • Lenat, D. R., & Barbour, M. T. (1994). Using benthic macroinvertebrate community structure for rapid, costeffective, water quality monitoring: Rapid bioassessment. In S. L. Loeb, & A. Spacie (Eds.), Biological monitoring of aquatic systems (pp. 187–215). Boca Raton: Lewis.

    Google Scholar 

  • Lorenz, A., Kirchner, L., & Hering, D. (2004). Electronic subsampling of macrobenthic samples: How many individuals are needed for a valid assessment result? Hydrobiologia, 516, 299–312.

    Article  Google Scholar 

  • Melo, A. S. (2005). Effects of taxonomic and numeric resolution on the ability to detect ecological patterns at a local scale using stream macroinvertebrates. Archiv für Hydrobiologie, 164, 309–323.

    Article  Google Scholar 

  • Moya, N., Tomanova, S., & Oberdorff, T. (2007). Initial development of a multi-metric index based on aquatic macroinvertebrates to assess streams condition in the Upper Isiboro-Sécure Basin, Bolivian Amazon. Hydrobiologia, 589, 107–116.

    Article  Google Scholar 

  • Mugnai, R., Oliveira, R. B. S., Carvalho, A. L., & Baptista, D. F. (2008). Adaptation of the Indice Biotico Esteso (IBE) for water quality assessment in rivers of Serra do Mar, Rio de Janeiro State, Brazil. Tropical Zoology, 21, 57–74.

    Google Scholar 

  • Nichols, S. J., & Norris, R. H. (2006). River condition assessment may depend on the sub-sampling method: Field live-sort versus laboratory sub-sampling of invertebrates for bioassessment. Hydrobiologia, 572, 95–213.

    Google Scholar 

  • Petkovska, V., & Urbanic, G. (2009). Effect of fixed-fraction subsampling on macroinvertebrate bioassessment of rivers. Environmental Monitoring and Assessment, (published online).

  • Pielou, E. C. (1977). Mathematical ecology (p. 385). New York: Wiley.

    Google Scholar 

  • Plafkin, J. L., Barbour, M. T., Porter, K. D., Gross, S. K., & Hughes, R. M. (1989). Rapid bioassessment protocols for use in streams and rivers: Benthic macroinvertebrates and fish. Washington, DC: EPA/440/4- 89/001, Office of Water, United States Environmental Protection Agency.

    Google Scholar 

  • Rosenberg, D. M., & Resh, V. H. (eds.). (1993). Freshwater biomonitoring and benthic macroinvertebrates. New York: Chapman and Hall.

    Google Scholar 

  • Somers, M. R., Reid, R. A., & David, S. M. (1998). Rapid biological assessments: How many individuals are enough? Journal of the North American Benthological Society, 17, 348–358.

    Article  Google Scholar 

  • Stroom, K. T., & Richards, C. (1999). Development of a biocriteria for streams of Minnesota’s Lake Superior watershed. Bulletin of the North American Benthological Society, 16, 202.

    Google Scholar 

  • Vinson, M. R., & Hawkins, C. P. (1996). Effects of sampling area and subsampling procedure on comparisons of taxa richness among streams. Journal of the North American Benthological Society, 15, 392–399.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Renata B. S. Oliveira.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oliveira, R.B.S., Mugnai, R., Castro, C.M. et al. Determining subsampling effort for the development of a rapid bioassessment protocol using benthic macroinvertebrates in streams of Southeastern Brazil. Environ Monit Assess 175, 75–85 (2011). https://doi.org/10.1007/s10661-010-1494-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10661-010-1494-4

Keywords