Abstract
For routine monitoring of pesticides, a multiresidue analysis through solid-phase extraction technique and using high-performance liquid chromatography (HPLC) in cotton seed cake (CSC) has been developed. Extraction of fortified samples was carried out with aqueous acetone under vacuum. The concentrated extract was loaded onto the solid-phase extraction units, preconditioned with acetonitrile. The extraction units were then washed with hexane and finally eluted with acetonitrile. The pesticide residues were determined using a multiresidue method by reversed-phase HPLC. The average percentage recoveries were found to range between 65.47% and 110% at spiking levels of 10 to 40 mg/kg. The method developed shows a healthy rate of recovery and can successfully be utilized for the extraction and screening of neonicotinoid residues in CSC. The detection limits for imidacloprid, acetamiprid, and thiacloprid using this method were found to be 5, 10, and 20 mg/kg, respectively.
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References
Ambrus, A., & Their, H. P. (1986). Application of multiresidue procedures in pesticides residues analysis. Journal of Pure and Applied Chemistry, 58, 1035–1062. doi:10.1351/pac198658071035.
Carretero, A. S., Cruces-Blanco, C., Duran, S. P., & Fernandez, A. G. (2003). Determination of Imidacloprid and its metabolite 6-chloronicotinic acid in greenhouse air by application of mivellar electrokinetic capillary chromatography with solid phase extraction. Journal of Chromatography. A, 1003, 189–195. doi:10.1016/S0021-9673(03)00835-5.
Di Muccio, A., Fidente, P., Barbini, D. A., Dommarco, R., Seccia, S., & Morrica, P. (2006). Application of solid phase extraction and liquid chromatography-mass spectrometry to the determination of Neonicotinoids pesticide residue in vegetables and fruits. Journal of Chromatography. A, 1108, 1–6. doi:10.1016/j.chroma.2005.12.111.
Font, G., Manes, J., Molto, J. C., & Pico, Y. (1993). Solid phase extraction in multiresidue pesticide analysis of water. Journal of Chromatography. A, 642, 135–161. doi:10.1016/0021-9673(93)80083-K.
Hirotaka, O., Mashiro, O., Kazuhiko, A., Yoko, K., & Shinjiro, H. (2002). Determination of neonicotinoid pesticide residue in vegetables and fruits with solid phase extraction and liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 51, 2501–2505.
Ishii, Y., Kobori, I., Araki, Y., Kurogochi, S., Iwayat, J. K., & Kagabug, S. (1994). HPLC determination of the new insecticide imidacloprid and its behavior in rice and cucumber. Journal of Agricultural and Food Chemistry, 42, 2917–2921. doi:10.1021/jf00048a051.
Kampioti, A. A., Da Cunha, A. C. B., De Alda, M. L., & Barcelo, D. (2005). Fully automated multianalyte determination of different classes of pesticide, at picogram per litre levels in water, by on line solid phase extraction- liquid chromatography-electrospray-tandom mass spectrometry. Analytical and Bioanalytical Chemistry, 382, 1815–1825. doi:10.1007/s00216-005-3332-z.
Pico, Y., Fernandez, M., Ruiz, M. J., & Guillermina, F. (2006). Current trends in solid phase based extraction techniques for the determination of pesticide in food and environment. Journal of Biochemistry, 02021, 1–16.
Stajnbaher, D., & Zupancic-Kralj, L. (2003). Multiresidue method for determination of 90 pesticides in fresh fruits and vegetables using solid-phase extraction and gas chromatography-mass spectrometry. Journal of Chromatography. A, 1015, 185–198. doi:10.1016/S0021-9673(03)01211-1.
Valéria, G., Azamela, M., Trebse, P., Erenc, G., & Franko, M. (2007). Determination of selected neonicotinoid insecticides by liquid chromatography with thermal lens spectrometric detection. Journal of Environmental Chemistry Letters, 5, 1610–3661.
Watanable, E., Baba, K., & Eun, H. (2007). Simultaneous determination of neonicotinoid insecticides in agricultural samples by solid-phase extraction clean up and liquid chromatography equipped with diode-array detection. Journal of Agricultural and Food Chemistry, 55, 3798–3804. doi:10.1021/jf063140m.
Yamamoto, I., & Yabuta, G. (1995). Molecular mechanism for selective toxicity of nicotinoids and neonicotinoids. Journal of Pesticide Science, 20, 33–40.
Ying, G., & Kookana Rai, S. (2004). Simultaneous determination of imidacloprid, thiacloprid, and thiamethoxam in soil and water by high-performance liquid chromatography with diode-array. Journal of Environmental Science and Health, 39, 737–746.
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Mohan, C., Kumar, Y., Madan, J. et al. Multiresidue analysis of neonicotinoids by solid-phase extraction technique using high-performance liquid chromatography. Environ Monit Assess 165, 573–576 (2010). https://doi.org/10.1007/s10661-009-0968-8
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DOI: https://doi.org/10.1007/s10661-009-0968-8