Abstract
The present study showed a possibility to use phenotypic and proteomic responses in rice plants as an in vivo biomarker to detect higher concentrations of ambient ozone (O3). The investigation was done on two cultivars of Indian rice using open top chambers ventilated with charcoal filtered air, ambient air, ambient air with 10 ppb O3 exposure and ambient air with 20 ppb O3 exposure at a rural site of Varanasi, India. Results showed that the magnitude of O3 induced specific type of foliar injury directly depends on the duration and concentration of O3 exposure. Even the internal protein profile of injured and normal leaf demonstrated a differential expression, which directly indicates towards the molecular basis of plant’s response against O3.
Similar content being viewed by others
References
Agrawal, G. K., Rakwal, R., Yonekura, M., Kubo, A., & Saji, H. (2002). Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings. Proteomics, 2, 947–959. doi:10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.CO;2-J.
Agrawal, M., Singh, B., Rajput, M., Marshall, F., & Bell, J. N. B. (2003). Effect of air pollution on peri-urban agriculture: A case study. Environmental Pollution, 126, 323–329. doi:10.1016/S0269-7491(03)00245-8.
Bell, J. N. B., & Ashmore, M. R. (1986). Design and construction of open top chambers and methods of filteration (equipment and cost). In Proceedings of II European open top chambers workshop. September 1906. Freiburg: CEC.
Biswas, D. K., Xu, H., Li, Y. G., Liu, M. Z., Chen, Y. H., Sun, J. Z., et al. (2008). Assessing the genetic relatedness of higher ozone sensitivity of modern wheat to its wild and cultivated progenitors/relatives. Journal of Experimental Botany, 59, 1–13. doi:10.1093/jxb/ern022.
Feng, Y. W., Komatsu, S., Furukawa, T., Koshiba, T., & Kohno, Y. (2008). Proteome analysis of proteins responsive to ambient and elevated ozone in rice seedlings. Agriculture Ecosystems & Environment, 125, 255–265. doi:10.1016/j.agee.2008.01.018.
Fiscus, E. L., Booker, F. L., & Burkey, K. O. (2005). Crop responses to ozone: Uptake, modes of action, carbon assimilation and partitioning. Plant, Cell & Environment, 28, 997–1011. doi:10.1111/j.1365-3040.2005.01349.x.
Gombert, S., Asta, J., & Seaward, M. R. D. (2006). Lichens and tobacco plants as complementary biomonitors of air pollution in the Grenoble area (Isere, Southeast France). Ecological Indicators, 6, 429–443. doi:10.1016/j.ecolind.2005.06.001.
Ishii, S., Marshall, F. M., & Bell, J. N. B. (2004). Physiological and morphological responses of locally grown Malaysian Rice Cultivars (Oryza sativa L.) to different ozone concentrations. Water, Air, and Soil Pollution, 155, 205–221. doi:10.1023/B:WATE.0000026528.86641.5b.
Jain, S. L., Arya, B. C., Kumar, A., Ghude, S. D., & Kulkarni, P. S. (2005). Observational study of surface ozone at New Delhi, India. International Journal of Remote Sensing, 26, 3515–3524. doi:10.1080/01431160500076616.
Klumpp, A., Ansel, W., Klumpp, G., Belluzzo, N., Calatayud, V., & Chaplin, N. (2002). EuroBionet: A Pan-European biomonitoring network for urban air quality assessment. Environmental Science and Pollution Research, 9, 199–203. doi:10.1007/BF02987489.
Laffray, X., Rose, C., & Garrec, J. P. (2007). Estimation of ozone concentration in a Valley of the Alps mountains based on Bel-W3 tobacco leaf injury. Water, Air, and Soil Pollution, 186, 29–42. doi:10.1007/s11270-007-9460-7.
Lowry, O. H., Rosenbourgh, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with folin phenol reagent. The Journal of Biological Chemistry, 193(1), 265–275.
Maggs, R., & Ashmore, M. R. (1998). Growth and yield responses of Pakistan rice (Oryza sativa L.) cultivars to O3 and NO2. Environmental Pollution, 103, 159–170. doi:10.1016/S0269-7491(98)00129-8.
Manning, W. J. (1997). The use of plants as bioindicators of ozone. In A. Bytnerowicz, M. J. Arbaugh, & S. L. Schilling (Eds.), Proceedings of international symposium on air pollution and climate change effects on forest ecosystems, 1996, Riverside, California. General Technical Report PSW-GTR-166 (pp. 19–26). Albany: Pacific Southwest Research Station, Forest Service, US Department of Agriculture.
Mauzerall, D. L., & Wang, X. (2001). Protecting agricultural crops from the effects of tropospheric ozone exposure: Reconciling science and standard setting in the United States, Europe and Asia. Annual Review of Energy and the Environment I, 26, 237–268.
Nali, C., Balducci, E., Frati, L., Paoli, L., Loppi, S., & Lorenzini, G. (2007). Integrated biomonitoring of air quality with plants and lichens: A case study on ambient ozone from central Italy. Chemosphere, 67(11), 2169–2176. doi:10.1016/j.chemosphere.2006.12.036.
Nali, C., Francini, A., & Lorenzini, G. (2006). Biological monitoring of ozone: The twenty-years Italian experience. Journal of Environmental Monitoring, 8, 25–32. doi:10.1039/b510303g.
Pleijel, H., Rerglen Eriksen, A., Danielsson, H., Bondesson, N., & Selldén, G. (2006). Differential ozone sensitivity in an old and a modern Swedish wheat cultivars—grain yield and quality, leaf chlorophyll and stomatal conductance. Environmental and Experimental Botany, 56, 63–71. doi:10.1016/j.envexpbot.2005.01.004.
Rai, R., Agrawal, M., & Agrawal, S. B. (2007). Assessment of yield losses in tropical wheat using open top chambers. Atmospheric Environment, 41, 9543–9554. doi:10.1016/j.atmosenv.2007.08.038.
Rao, M. V., Hale, B. A., & Ormrod, D. P. (1995). Amelioration of ozone-induced oxidative damage in wheat plants grown under high carbon dioxide. Plant Physiology, 109, 421–432.
Tiwari, S., & Agrawal, M. (2006). Evaluation of ambient air pollution impact on carrot plants at a suburban site using open top chamber. Environmental Monitoring and Assessment, 119, 15–30. doi:10.1007/s10661-005-9001-z.
Tiwari, S., Agrawal, M., & Manning, W. J. (2005). Assessing the impact of ambient ozone on growth and productivity of two cultivars of wheat in India using three rates of application of ethylenediurea (EDU). Environmental Pollution, 138, 153–163. doi:10.1016/j.envpol.2005.02.008.
Tiwari, S., Rai, R., & Agrawal, M. (2008). Annual and seasonal variations in tropospheric ozone concentrations around Varanasi. International Journal of Remote Sensing, 29(15–16), 4499–4514.
Vingarzan, R. (2004). A review of surface ozone background levels and trends. Atmospheric Environment, 38, 3431–3442. doi:10.1016/j.atmosenv.2004.03.030.
Wahid, A. (2006). Influence of atmospheric pollutants on agriculture in developing countries: A case study with three new wheat varieties in Pakistan. The Science of the Total Environment, 371, 304–313. doi:10.1016/j.scitotenv.2006.06.017.
Wang, X., Manning, W., Feng, Z., & Zhu, Y. (2007). Ground-level ozone in China: Distribution and effect on crop yields. Environmental Pollution, 147, 394–400. doi:10.1016/j.envpol.2006.05.006.
Yonekura, T., Shimada, T., Miwa, M., Arzate, A., & Ogawa, K. (2005). Impacts of tropospheric ozone on growth and yield of rice. Journal of Agricultural Meteorology, 60, 1045–1048.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sarkar, A., Agrawal, S.B. Identification of ozone stress in Indian rice through foliar injury and differential protein profile. Environ Monit Assess 161, 205–215 (2010). https://doi.org/10.1007/s10661-008-0738-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10661-008-0738-z