Abstract
Fifty seven isolates of thermotolerant E. coli were recovered from 188 drinking water sources, 45 (78.9%) were typable of which 15 (26.3%) were pathogenic serotypes. Pathogenic serogroup obtained were 04 (Uropathogenic E. coli, UPEC), 025 (Enterotoxigenic E. coli, ETEC), 086 (Enteropathogenic E. coli, EPEC), 0103 (Shiga-toxin producing E. coli, STEC), 0157 (Shiga-toxin producing E. coli, STEC), 08 (Enterotoxigenic E. coli, ETEC) and 0113 (Shiga-toxin producing E. coli, STEC). All the pathogenic serotypes showed resistance to bacitracin and multiple heavy metal ions. Resistance to streptomycin and co-trimazole was detected in two strains whereas resistance to cephaloridine, polymixin-B and ampicillin was detected in one strain each. Transfer of resistances to drugs and metallic ions was observed in 9 out of 12 strains studied. Resistances to bacitracin were transferred in all nine strains. Among heavy metals resistance to As3+ followed by Cr6+ were transferred more frequently.
Similar content being viewed by others
References
APHA. (1992). Standard methods for the examination of water and wastewater (18th ed.). Washington, D.C.: American Public Health Association.
Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Twick, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493.
Bolton, F. J., Crozier, L., & Williamson, J. K. (1996). Isolation of E. coli 0157 from raw meat products. Letters in Applied Microbial, 23, 317–321.
Bonnet, R., Souweine, B., Gauthier, G., Rich, C., Livrelli, V., Sirot, J., et al. (1998). Non-0157:H 7 Stx2-producing E. coli strain associated with sporadic cases of hemolytic–uremic syndrome in adults. Journal of Clinical Microbiology, 36, 1777–1780.
Boyce, T. G., Swerdlow, D. L., & Griffin, P. M. (1995). Current concepts: E. coli 0157:H7 and the hemotypic uremic syndrome. New England Journal of Medicine, 333, 364.
Cervantes-vega, C., Chanez, J., Cordona, N. A., de la More, P., & Velasco, J. A. (1986). Resistance to metals by Pseudomonas aeruginosa clinical isolates. Microbios, 48, 159.
Chattopadhya, D., & Basu, S. (1986). Modified scheme for identification of coliform organism in drinking water. Indian Journal of Medical Research, 83, 152.
Day, N. P., Scotland, S. M., Cheasty, T., & Rowe, B. (1983). E. coli 0157: H7 associated with human infections in the United Kingdom. Lancet, 1, 825.
Griffin, P. M. (1999). Escherichia coli 0157: H7 and other enterohaemorrhagic E. coli. In M. J. Blaser, P. D. Smith, J. I. Ravdin, H. B. Greenberg & R. L. Guerrant (Eds.), Infections of the gastrointestinal Tract I (p. 739). New York: Raven.
Kaper, J. B. (1994). Molecular pathogenesis of enteropathogenic Escherichia coli. In V. L. Miller, J. B. Kaper, D. A. Portnoy & R. R. Iceberg (Eds.), Molecular genetics of bacterial pathogenesis (p. 173). Washington: ASM.
Kita, E., Yunou, Y., Kurioka, T., Harada, H., Yoshikawa, S., Mikasa, K., et al. (2000). Pathogenic mechanism of mouse brain damage caused by oral infection with shiga toxin producing Escherichia coli 0157: H7. Infection and Immunity, 68, 1207.
Lindenthal, C., & Elinghorst, E. A. (2001). Enterotoxigenic Escherichia coli TibA glycoprotein adheres to human intestine epithelial cells. Infection and Immunity, 69, 52.
Maniatis, T., Fritsch, E. F., & Sambrook, J. (1982). Molecular cloning, a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory.
Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S. (1976). Simple agarose gel electrophoretic methods for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology, 127, 1529.
Muhldorfer, I., & Hacker, J. (1994). Genetic aspect of E. coli virulence. Microbial Pathogenesis, 16, 171.
Nataro, J. P., & Kapar, J. B. (1998). Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142.
Oswald, E., Schmidt, H., Moradito, S., Karch, H., Marches, O., & Caprioli, A. (2000). Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: Characterization of a new intimin variant. Infection and Immunity, 68, 64.
Pichel, M., Binsztein, N., & Viboud, G. (2000). Cs22, a novel human enteroxigenic Escherichia coli adhesin, is related to Cs15. Infection and Immunity, 68, 3280.
Ramteke, P. W. (1995). Comparison of standard most probable number method with three alternate tests for detection of bacteriological water quality indicators. Environmental Toxicology and Water Quality, 10, 173–178.
Ramteke, P. W., Bhattacharjee, J. W., Pathak, S. P., & Kalra, N. (1992). Evaluation of coliforms as indicators of water quality in India. Journal of Applied Bacteriology, 72, 352–356.
Tewari, S., Ramteke, P. W., & Garg, S. K. (2003). Evaluation of simple microbial tests for detection of fecal coliforms directly at 45.5°C. Environmental Monitoring and Assessment, 85, 191–198.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ramteke, P.W., Tewari, S. Serogroups of Escherichia coli from Drinking Water. Environ Monit Assess 130, 215–220 (2007). https://doi.org/10.1007/s10661-006-9390-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10661-006-9390-7