Skip to main content
SpringerLink
Log in

MOVEMENT BEHAVIOUR OF MEDAKA (Oryzias latipes) IN RESPONSE TO SUBLETHAL TREATMENTS OF DIAZINON AND CHOLINESTERASE ACTIVITY IN SEMI-NATURAL CONDITIONS

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract.

Behavioural changes of medaka (Oryzias latipes) treated with an anticholinesterase insecticide, diazinon (0.1 mg L- 1), were continuously observed for 4 days in semi-natural conditions. Although variations occurred in individual specimens, the movement tracks appeared differently with typical short-range movement with irregular turns and shaking after the treatments. Eight movement patterns frequently observed before and after the treatments were selected, and the variables characterising the movement patterns were compared quantitatively. The variables were clearly differentiated when the movement patterns were correspondingly matched before and after the treatments (e.g., vertical movements, horizontal movements, etc). Meander and stop duration were highly different among the selected movement patterns. Additionally, different degree of toxic response behaviours could also be detected by quantitative characterisation of the variables. Response behaviour was confirmed with toxicological experiments that show the decrease in the acetylcholine esterase activity in the head and body of specimens. Quantitative investigations on the variables of the movement tracks suggested the usefulness of response behaviour as a monitoring tool for environmental assessment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abgrall, P.,Rangeley, R. W.,Burridge, L. E. and Lawton, P.: 2000, ‘Sublethal effects of azamethiphos on shelter use by juvenile lobsters (Homarus americanus)’, Aquaculture 181, 1–10.

    Article  CAS  Google Scholar 

  • Baganz, D.,Staaks, G. and Steinberg, C.: 1998, ‘Impact of the cyanobacteria toxin, microcystin-LR on behaviour of zebrafish, Danio rerio’, Wat. Res. 32, 948–952.

    Article  CAS  Google Scholar 

  • Barabas, K.: 1998, Environmental Health Criteria 198, World Health Organization Geneva, Switzerland.

    Google Scholar 

  • Blübaum-gronau, E.,Hoffmann, M.,Spieser, O. H. and Krebs, F.: 1994, ‘The ‘Koblenz behavioural fish test’, an automated biotest based on the video processing system BehaviQuant® for monitoring of water quality’, Schrifenreihe des Vereins für Boden-, Wasser- und Lufthygiene 93, 87–117.

    Google Scholar 

  • Burridge, L. E.,Haya, K.,Waddy, S. L. and Wade, J.: 2000, ‘The lethality of anti-sea lice formulations Salmosan® (Azamethiphos) and Excis® (Cypermethrin) to stage IV and adult lobsters (Homarus americanus) during repeated short-term exposures’, Aquaculture 182, 27–35.

    Article  CAS  Google Scholar 

  • Collins, R. D.,Gargesh, R. N.,Maltby, A. D.,Roggero, R. J.,Tourtellot, M. K. and Bell, W. J.: 1994, ‘Innate control of local search behaviour in the house fly, Musca domestica’, Physiol. Entomol. 19, 165–172.

    Article  Google Scholar 

  • Drai, A.,Benjamini, Y. and Golani, I.: 2000, ‘Statistical discrimination of natural modes of motion in rat exploratory behaviour’,J. Neurosci. Meth. 96, 119–131.

    Article  CAS  Google Scholar 

  • Dutta, H.,Marcelino, J. and Richmonds, Ch.: 1992, ‘Brain acetylcholinesterase activity and optomotor behaviour in bluegills, Lepomis macrochirus exposed to different concentrations of diazinon’, Arch. Intern. Physiol. Biochim. Biophys. 100, 331–334.

    Article  CAS  Google Scholar 

  • Ellman, G. L.,Courtney, K. D.,Andres, V. Jr. and Featherstone, R. M.: 1961, ‘A new and rapid colorimetric determination of acetylcholinesterase activity’, Biochem. Pharmacol. 7, 88.

    Article  CAS  Google Scholar 

  • Ferslew, K. E.,Hagardorn, A. N. and McCormick, W. F.: 1992, ‘Poisoning from oral ingestion of carbofuran (Furadan 4F), a cholinesterase-inhibiting carbamate insecticide, and its effects on cholinesterase activity in various biological fluids’, J. Forensic. Sci. 37, 337–344.

    CAS  Google Scholar 

  • Galgani, F. and Bocquene, G.: 1990, ‘In vitro inhibition of acetylcholinesterase from four marine species by organophosphates and carbamates’, Bull. Environ. Contam. Toxicicol. 45, 243–249.

    Article  CAS  Google Scholar 

  • Gray, M. A.,Teather, K. L. and Metcalfe, C. D.: 1999, ‘Reproductive success and behaviour of Japanese Medaka (Oryzias latipes) exposed to 4-tert-octylphenol’, Environ. Toxicol. Chem. 18, 2587–2594.

    Article  CAS  Google Scholar 

  • Gupta, R. C.: 1994, ‘Carbofuran toxicity’, J. Toxicol. Environ. Health 43, 383–418.

    Article  CAS  Google Scholar 

  • Ibrahim, W. L. F.,Furu, P.,Ibrahim, A. M. and Christensen, N. Ø.: 1992, ‘Effect of the organophosphorous insecticide, chlorpyrifos (Dursban), on growth, fecundity and mortality of Biomphalaria alexandrina and on the production of Schistosoma mansoni cercariae in the snail’, J. Helminthol. 66, 79–88.

    Article  CAS  Google Scholar 

  • Kim, J. S.,Koh, S. C.,Lee, S. K. and Chon, T. S.: 1999, ‘Regulation of acetylcholine esterase and neurotransmitters in Oryzias latipes by diazinon’, Kor. J. Environ. Toxicol. 14, 81–85.

    Google Scholar 

  • Kozolvskaya, V. I. and Mayer, F. L. Jr.: 1984, ‘Brain acetylcholinesterase and backbone collagen in fish intoxicated with organophosphate pesticides’, Great Lake Res. 10, 261–266.

    Article  Google Scholar 

  • Kwak, I. S.,Chon, T. S.,Kang, H. M.,Chung, N. I.,Kim, J. S.,Koh, S. C.,Lee, S. K. and Kim,Y. S.: 2002, ‘Pattern recognition of the movement tracks of medaka (Oryzias latipes) in response to sub-lethal treatments of an insecticide by using artificial neural networks’, Environ. Pollut. 120, 671–681.

    CAS  Google Scholar 

  • Lee, S. E. and Lee, S. K.: 1996, ‘Biological sensors for detecting aquatic toxicity of hazardous chemicals using respiratory and movement responses of carp, Cyprinus capio’, J. Korean Soc. Water Qual. 12, 119–126.

    Google Scholar 

  • Lemly, A. D. and Smith, R. J.: 1986, ‘A behavioural assay for assessing effects of pollutants of fish chemoreception’, Ecotoxicol. Environ. Saf. 11, 210–218.

    Article  CAS  Google Scholar 

  • Lorenz, R.,Spieser, O. H. and Steinberg, C.: 1995, ‘New ways to ecotoxicology: Quantitative recording of behaviour of fish as toxicity endpoint’, Acta Hydrochim. Hydrobiol. 23, 197–201.

    Article  CAS  Google Scholar 

  • Lowry, O. H.,Rosebrough, N. J.,Farr, A. L. and Randall, R. J.: 1951, ‘Protein measurement with the Folin phenol reagent’, J. Biol. Chem. 193, 265.

    CAS  PubMed  Google Scholar 

  • Michels, E.,Leynen, M.,Cousyn, C.,De Meester, L. and Ollievier, F.: 1999, ‘Phototactic behaviour of Daphnia as a tool in the continuous monitoring of water quality: Experiments with a positively phototacticDaphnia magna clone’, Water Res. 33, 401–408.

    Article  CAS  Google Scholar 

  • Michels, E.,Semsari, S., Bin, C. and De Meester, L.: 2000, ‘Effects of sublethal doses caumium on the phototactic behaviour of Daphnia magna’, Ecotoxicol. Environ. Saf. 47, 261–265.

    Article  CAS  Google Scholar 

  • Moore, A. and Waring, C. P.: 1996, ‘Sublethal effects of the pesticide diazinon on olfactory function in mature male Atlantic salmon parr’, J. Fish Biol. 48, 758–775.

    Article  Google Scholar 

  • Oshima, Y.,Kang, I. K.,Kobayashi, M.,Nakayama, K.,Imada, N. and Honjo., Tsuneo.: 2003, ‘Suppression of sexual behaviour in male Japanese medaka (Oryzias latipes) exposed to 17β-estradiol’, Chemosphere 50, 429–436.

    Article  Google Scholar 

  • Roast, S. D.,Widdows, J. and Jones, M. B.: 2000, ‘Disruption of swimming in the hyperbenthic mysid Neomysis integer (Peracarida: Mysidacea) by the organophosphate pesticide chlorpyrifos’, Aquat. Toxicol. 47, 227–241.

    Article  CAS  Google Scholar 

  • Schal, C.,Tobin, T. R.,Surber, J. L.,Vogel, G.,Tourtellot, M. K.,Leban, R. A.,Sizemore, R. and Bell, W. J.: 1983, ‘Search strategy of sex pheromone-stimulated male German cockroaches’, J. Insect Physiol. 29, 575–579.

    Article  Google Scholar 

  • Smith, G. J. (ed.): 1993, ‘Toxicology and Pesticide Use in Relation to Wildlife’, in Organophosphorus and Carbamate Compounds. CRC Press, Boca Raton, FL.

    Google Scholar 

  • Staaks, G.: 1996, ‘Experimental studies on temperature preference behaviour of juvenile cyprinids’, Limnologica 26, 165–177.

    Google Scholar 

  • Staaks, G.,Kirschbaum, F. and Willot, P.: 1999, ‘Experimental studies on thermal behaviour and diurnal activity rhythms of juvenile European sturgeon (Acipenser sturio)’, J. Appl. Ichthyol. 15, 243–247.

    Article  Google Scholar 

  • St-Amand, L.,Gagnon, R.,Packard, T. T. and Savenkoff, C.: 1999, ‘Effects of inorganic mercury on the respiration and the swimming activity of shrimp larvae, Panaslus borealis’, Compar. Biochem. Physiol. Part C 112, 33–43.

    Google Scholar 

  • Tchernichovski, O.,Benjamini, Y. and Golani, I.: 1998, ‘The dynamics of long-term exploration in the rat. Part I. A phase-plane analysis of the relationship between location and velocity’, Biol. Cybern. 78, 423–432.

    Article  CAS  Google Scholar 

  • Tchernichovski, O. and Benjamini, Y.: 1998, ‘The dynamics of long-term exploration in the rat. Part II. An analytical model of the kinematic structure of rat exploratory behaviour’, Biol. Cybern. 78, 433–440.

    Article  CAS  Google Scholar 

  • Yamamoto, T.: 1967, ‘Medaka’, in Methods in Developmental Biology. T.Y. Crowell Co., New York, pp. 101–110.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biological Sciences, Pusan National University, Pusan, Korea

    TAE-SOO CHON, NAMIL CHUNG & INN-SIL KWAK

  2. Department of Animal Science and Biotechnology, Kyungpook National University, Taegu, Korea

    JONG-SANG KIM

  3. Department of Marine Environmental Engineering, Korea Maritime University, Pusan, Korea

    SUNG-CHEOL KOH

  4. Toxicology Research Center, Korea Research Institute of Chemical Technology, Taejon, Korea

    SUNG-KYU LEE

  5. Korean Inter-University Institute of Ocean Science, Pusan, Korea

    JOO-BAEK LEEM

  6. Division of Computer Science and Engineering, Pusan National University, Pusan, Korea

    EUI YOUNG CHA

Authors
  1. TAE-SOO CHON
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. NAMIL CHUNG
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. INN-SIL KWAK
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. JONG-SANG KIM
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. SUNG-CHEOL KOH
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. SUNG-KYU LEE
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. JOO-BAEK LEEM
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. EUI YOUNG CHA
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to TAE-SOO CHON.

Rights and permissions

Reprints and permissions

About this article

Cite this article

CHON, TS., CHUNG, N., KWAK, IS. et al. MOVEMENT BEHAVIOUR OF MEDAKA (Oryzias latipes) IN RESPONSE TO SUBLETHAL TREATMENTS OF DIAZINON AND CHOLINESTERASE ACTIVITY IN SEMI-NATURAL CONDITIONS. Environ Monit Assess 101, 1–21 (2005). https://doi.org/10.1007/s10661-005-9125-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10661-005-9125-1

Keywords:

Search

Navigation