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Evaluation Of Ambient Air Pollution Impact On Carrot Plants At A Sub Urban Site Using Open Top Chambers

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Abstract

The present experiment was done to evaluate the impact of ambient air pollution on carrot (Dacus carotavar. Pusa Kesar) plants using open top chambers (OTCs) ventilated with ambient (NFCs) or charcoal filtered air (FCs) at a suburban site of Varanasi, India. Various morphological, physiological and biochemical characteristics of the plants were studied at different growth stages. Air monitoring data clearly showed high concentrations of SO2, NO2and O3in the ambient air of study site. SO2and NO2concentrations were higher during early growth stages of carrot, whereas O3concentration was highest during later growth stages. Filtration of air has caused significant reductions in all the three pollutant concentrations in FCs as compared to NFCs.Plants growing in FCs showed significantly higher photosynthetic rate, stomatal conductance, water use efficiency and variable fluorescence as compared to plants growing in NFCs. Protein content also showed a similar pattern, however, lipid peroxidation, ascorbic acid content and peroxidase activity were higher in plants growing in NFCs as compared to FCs. Shoot length, number of leaves per plant, leaf area and root and shoot weight increased significantly upon filtration of ambient air. Total nitrogen decreased significantly in root, but increased significantly in shoot of plants grown in NFCs. Total P, Mg, Ca and K contents decreased significantly in plants grown in NFCs as compared to FCs. The individual pollutant concentrations were below threshold for plant injury, but the combined effect of all the three seems to act synergistically in causing greater adverse impact on dry weight and physiology of carrot plants. The study clearly indicates that air pollutants are high enough in the ambient air to cause significant unfavorable impact on carrot plants. The work further supports the usefulness of OTCs for assessing air pollution damage under field conditions in developing countries.

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References

  • Adams, R. M., Glter, J. D. and McCarl, B. A.: 1988, ‘The NCLAN economic assessment: Approach and findings and implications’, in: W. W. Heck, O. C. Taylor and D. T. Tingey (eds), Assessment of crop loss from air pollutants. Elsevier, London, pp. 473–504.

    Google Scholar 

  • Agrawal, M. and Agrawal, S. B.: 1990, ‘Effects of ozone exposure on enzymes and metabolites of nitrogen metabolism’, Sci. Hort. 43, 169–177.

    Article  CAS  Google Scholar 

  • Agrawal, M., Singh, B., Rajput, M., Marshall, F. and Bell, J. N. B.: 2003, ‘Effects of air pollution on peri urban agriculture: A case study’, Environ Pollut. 126, 323–329.

    Article  CAS  Google Scholar 

  • Allen, S. E., Grimshaw, H. M., Parkinson, J. A. and Quarmby, C.: 1974, ‘Chemical Analysis of Ecological Materials’, Blackwell Scientific Publications, Osney Mead, Oxford, UK.

    Google Scholar 

  • Atkinson, C. J., Robe, S. V. and Winner, W. E.: 1988, ‘The relationship between changes in photosynthesis and growth for radish plants fumigated with SO2 and NO2’, New Phytol 110, 173–184.

    Article  CAS  Google Scholar 

  • Bell, J. N. B. and Ashmore, M. R.: 1986, ‘Design and construction of open top chambers and methods of filtration (equipments and cost)’, in: Proceedings of II European Open Top Chambers Workshop, September 1986, Freiburg, CEC Brussels.

  • Black, V. J., Ormond, D. P. and Unsworth, M. H.: 1982, ‘Effects of low concentration of O3 singly and in combination with SO2 on net photosynthesis rates of Vicia faba L’, J. Exp. Bot. 33, 1302–1311.

    CAS  Google Scholar 

  • Bonte, J., Cantuel, J., Galanp, S. and Longuet, P.: 1988, ‘Effects of ambient O3 on yield and physiological parameters of bean (Phaseolus vulgaris, cultivar Lit)’ in: J. Bonte and P. Mathy (eds), The European Community Project on Open Top Chambers. Results on Agricultural Crops for 1987–1988, Air Pollution Report Series no. 19, Commission of the European Communities, Brussels, Belgium, pp. 26–42.

  • Bray, H. G. and Thorpe, W. Y.: 1954, ‘Analysis of phenolic compounds of interests in metabolism’, in: D. Click (ed), Methods of Biochemical Analysis, Interscience Publication Inc. New York, 1, 27–52.

  • Britton, C. and Mehley, A. C.: 1955, in: S. P. Colowick and N. O. Kalpan (eds), Methods in enzymology, Academic Press Inc. New York, 2, 764.

  • Bull, J. N. and Mansfield, T. A.: 1974, ‘Photosynthesis in leaves exposed to SO2 and NO2’, Nature 250, 443–444.

    Article  CAS  Google Scholar 

  • Calatayud, A., Ramirez, J. W., Iglesias, D. J. and Barreno, E.: 2002, ‘Effect of O3 on photosynthetic CO2 exchange, chlorophyll a fluorescence and antioxidant systems in lettuce leaves’, Physiol Plant 116, 308–316.

    Article  CAS  Google Scholar 

  • Castillo, F. J., Penel, C. L. and Greppin, H.: 1984, ‘Peroxidase release induced by O3 in Sedum album leaves: Involvement of Ca++’, Plant Physiol 74, 846–851.

    CAS  Google Scholar 

  • Darrall, N. M.: 1989, ‘The effect of air pollutants on physiological processes in plants’, Plant Cell Environ. 12, 1–30.

    Article  CAS  Google Scholar 

  • Deepak, S. S. and Agrawal, M.: 2001, ‘Influence of elevated CO2 on sensitivity of two soybean cultivars to SO2’, Environ. Exp. Bot. 46, 81–91.

    Article  CAS  Google Scholar 

  • Dekok, L. J.: 1990, ‘Sulphur metabolism in plants exposed to atmospheric sulphur’, in: H. Rennerberg, C. Brunold, L. J. Dekok and I. Stulen (eds), Sulphur Nutrition and Sulphur Assimilation in Higher Plants: Fundamental, Environmental and Agricultural Aspects, SBS Academic Publishing, The Hauge, The Netherlands, pp. 125–138.

    Google Scholar 

  • Dhindsa, R. S., Plumb- Dhindhsa, P. and Thorpe T. A.: 1982, ‘Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase’, J. Exp. Bot. 32, 93–101.

    Google Scholar 

  • Duxbury, A. C. and Yentsch, C. S.: 1956, ‘Plankton pigment monographs’, J. Marine. Res. 15, 91–101.

    Google Scholar 

  • Hassan, I. A., Ashmore, M. R. and Bell J. N. B.: 1995, ‘Effect of ozone on radish and turnip under Egyptian field conditions’, Environ. Pollut. 89, 107–114.

    Article  CAS  Google Scholar 

  • Heath, R. L. and Packer, L.: 1968, ‘Photoperoxidation in isoloted chloroplasts’, Arch. Biochem. Biophys. 125, 189–198.

    Article  CAS  Google Scholar 

  • Heggestad, H. E., Heagle, A. S., Bennett. J. H. and Koch E. J.: 1980, ‘The effects of photochemical oxidants on the yield of snap beans’, Atmos. Environ. 14, 317–326.

    Article  CAS  Google Scholar 

  • Howell, R,K.: 1974, ‘Phenols, ozone and their involvement in the physiology of plant injury’, in: M. Dugger (ed), Air Pollution Related to Plant Growth, A.C.S. Symposium Series 3. Washington DC, pp. 94–105.

  • Hunt, R.: 1982, Growth curves. Edward Arnold (Publishers) Ltd. London.

    Google Scholar 

  • Huve, K., Dittrich, A., Kindermann, G. and Herber, U.: 1995, ‘Detoxification of SO2 in conifers differing in SO2 tolerance. A comparision of Picea Abies, Picea Pungens and Pinus Sylvestris’, Planta 195, 578–585.

    Google Scholar 

  • Jager, H. J., Unsworth, M., De Temmerman, L. and Mathy, P.: 1994, ‘Effects of air pollution on agricultural crops in Europe, Air Pollution Report 46. CEC, Brussels.

  • Keller, T. and Jager, H. J.: 1980, ‘Der einflux bodenburtiger sulfationen auf den schwefelgehalt SO2- begaster assimilations rgane von waldbaumarten’, Angew Botany 54, 77–89.

    CAS  Google Scholar 

  • Keller, T. and Schwager, H.: 1977, ‘Air pollution and ascorbic acid’, European J. Pathol. 7, 338–350.

    CAS  Google Scholar 

  • Kelly, J. M., Taylor, G. E., Edwards, N. T., Adams, M. B., Edwards, G. S. and Friend, A. L.: 1993, ‘Growth physiology and nutrition of loblolly pine seedlings stressed by O3 and acid precipitation: A summary of the ropis-south project’, Water Air and Soil Pollut. 69, 363–391.

    Article  CAS  Google Scholar 

  • Krause, G. H., Virgo, A. and Winter, K.: 1995, ‘High susceptibility to photoinhibition of young leaves of tropical forest trees’, Planta 197, 583–591.

    Article  CAS  Google Scholar 

  • Krupa, S. V., Grunhage, L., Jager, H. J., Nosel, M., Legge, A. H. and Hanewald, K.: 1995, ‘Ambient ozone and adverse crop response: A unified view of cause and effects’, Environmental Pollution 87, 119–126.

    Article  CAS  Google Scholar 

  • Lee, E. H., Jersey, J. H., Gifford, C. and Bennet, J.: 1984, ‘Differential O3 tolerance in soybean and snapbeans: Analysis of ascorbic acid in O3 susceptible and O3 resistant cultivars by high performance liquid chromatography’, Environ. Exp. Bot. 24, 331–334.

    Article  CAS  Google Scholar 

  • Lowry, O. H., Farr, A. L., Rosenbrough and Randall, R. J.: 1951, ‘Protein measurement with folin reagent’, J. Biological. Chem. 193, 265–275.

    CAS  Google Scholar 

  • Machlachlan, S. and Zalik, S.: 1963, ‘Plastid structure, chlorophyll concentration and free amino acid composition of a chlorophyll mutant of barley’, Can. J. Bot. 41, 1053–1062.

    Article  Google Scholar 

  • McLeod, A. R., Roberts, T. M., Alexander, K. and Cribb, D. M.: 1988, ‘Effects of open air fumigation with sulphur dioxide on the growth and yield of winter barley’, New Phytol. 109, 67–78.

    Article  CAS  Google Scholar 

  • Merrymann, E. L., Spicer, C. W. and Levy, A.: 1973, ‘Evaluation of arsenate modified Jacobs Hochheiser procedure’, Environ. Sci. Tech. 7, 1056–1059.

    Article  Google Scholar 

  • Pandey, J. and Agrawal, M.: 1994, ‘Evaluation of air pollution in seasonally dry topical urban environment using three woody perennials’, New Phytol. 126, 53–61.

    Article  CAS  Google Scholar 

  • Paulden- Muller, S., Saxe, H. and Leverenz, J. W.: 1999, ‘Responses to O3 in 12 provenances of European beech (Fagus sylvestica): genotypic variations and chamber effects on photosynthesis and dry matter partitioning’, New Phytol. 144, 261–273.

    Article  Google Scholar 

  • Peters, J. L., Castillo, F. J. and Heath, R. L.: 1988, ‘Alteration of extracellular enzymes in Pinto bean leaves upon exposure to air pollutants, O3 and SO2’, Plant Physiol. 89, 159–164.

    Google Scholar 

  • Pleijel, H., Skarby, L., Wallin, G. and Sellden, G.: 1991, ‘Yield and grain quality of spring wheat (T. aestivum Drabant) exposed to different concentrations of ozone in open top chambers’, Environ. Pollut. 69, 151–168.

    Article  CAS  Google Scholar 

  • Rajput, M. and Agrawal, M.: 2004, ‘Physiological and yield responses of pea plants to ambient air pollution’, Indian J. Plant. Physiol. 9, 9–14.

    CAS  Google Scholar 

  • Ranieri, A., D’llrso, G., Nali, C., Lorenzini, G. and Soldatini, G. F.: 1996, ‘Ozone stimulates apoplastic antioxidant systems in pumpkin leaves’, Physiol. Plant 97, 381–387.

    Article  CAS  Google Scholar 

  • Reiling, K. and Davison, A. W.: 1992, ‘Effects of short O3 exposure given at different stages in the development of Plantago major L’, New phytol. 121, 643–647.

    Article  CAS  Google Scholar 

  • Rossum, J. R. and Vallaruz, P. 1961, ‘Suggested methods of turbiditimetric determination of sulphate in water’. J. Amer. Water. Work. Assoc. 53, 873.

    CAS  Google Scholar 

  • Sandhu, R. and Gupta, G.: 1989, ‘Effect of nitrogen dioxide on growth and yield of black turtle bean (Phaseolus vulgaris L.) cv. Domino’, Environ. Pollut. 59, 337–344.

    Article  CAS  Google Scholar 

  • Saxe, H.: 1991, ‘Photosynthesis and stomatal responses to polluted air, and the use of physiological and biochemical responses for early detection and diagnostic tools’, in: J. A. Callow (ed), Advances in botanical research. Academic Press, London, UK, 18, 1–128.

  • Shimazaki, K., Sakai, T., Kondo, N. and Sugahara, K.: 1980, ‘Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2-fumigated leaves of spinach’, Plant cell Physiol 21, 1193–1204.

    CAS  Google Scholar 

  • Spence, R. D., Rykeil, E. J. and Sharpe, P. J. H.: 1990, ‘Ozone alters carbon allocation in loblolly pine: assessment with carbon-11 labelling’, Environ. Pollut. 64, 93–106.

    Article  CAS  Google Scholar 

  • TERG: 1988, The Effects of Acid Deposition on the Terrestrial Environment in the United Kingdom. UK Terrestrial Effects Review Group, 1st report. HMSO, London.

  • Varshney, R. K. and Varshney, C. K.: 1984, ‘Effects of SO2 on ascorbic acid in crop plants’, Environ. Pollut. 35, 285–290.

    Article  CAS  Google Scholar 

  • Wahid, A., Maggs, R., Shamsi, S. R. A., Bell, J. N. B and Ashmore, M. R.: 1995(a), ‘Air pollution and its impacts on wheat yield in Pakistan Punjab’, Environ. Pollut. 88, 147–154.

    Article  CAS  Google Scholar 

  • Wahid, A., Maggs, R., Shamsi, S. R. A., Bell, J. N. B. and Ashmore, M. R.: 1995(b), ‘Effect of air pollution on rice yield in Pakistan Punjab’, Environ. Pollut. 90, 323–329.

    Article  CAS  Google Scholar 

  • Wellburn, A. R., Higginson, C., Robinson, D. and Walmsley, C.: 1981, ‘Biochemical explanations of more than additive levels of SO2 and NO2 upon plants’, New Phytol. 88, 223–237.

    Article  CAS  Google Scholar 

  • West, P. W. and Gaeke, G. C.: 1956, ‘Fixation of SO2 as sulphomercurate (II) and subsequent colorimetric estimation’, Anal. Chem. 28, 1816–1819.

    Article  CAS  Google Scholar 

  • Williams, J. D. H., Syers, J. K., Walker, T. W. and Rex, R. W.: 1970, ‘A comparison of methods for the determination of soil organic phosphorous’, Soil Science 110, 13–18.

    Article  CAS  Google Scholar 

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Tiwari, S., Agrawal, M. & Marshall, F.M. Evaluation Of Ambient Air Pollution Impact On Carrot Plants At A Sub Urban Site Using Open Top Chambers. Environ Monit Assess 119, 15–30 (2006). https://doi.org/10.1007/s10661-005-9001-z

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