Abstract
Compost teas are watery fermented extracts with proven suppressive effects against plant pathogens. Compost teas were prepared from six different compost sources and assayed for in vitro effects against Botrytis cinerea growth. In vivo, weekly foliar application of compost teas on tomato plants and a tomato leaflet trial with undiluted and 10 × diluted teas were conducted to assess effects on grey mould caused by B. cinerea. Results showed that all tested compost teas inhibited B. cinerea growth when unsterilized. Heat- and cold-sterilization of compost teas reduced or eliminated inhibitory effects, suggesting that the presence of microorganisms is essential for inhibitory efficacy. When used as foliar sprays, bovine manure (BOV), shrimp (SHR), and garden-leaves-straw (GLS) compost teas effectively reduced disease severity for six weeks. In leaflet trials, BOV, GLS, and garden waste (GAR) compost teas caused the greatest delay in grey mould lesion expansion. Moreover, BOV, GLS, and, in particular, SHR compost teas were equally effective in undiluted and diluted form. Microbial characterization showed varying microbial compositions across all compost teas, with notably large populations of fungi or oomycetes and Pseudomonas spp. in GLS. There was no clear relationship between the source of compost (manure, other animal by-product, solely plant-based) and efficacy against grey mould. Results also indicate that the abundance of microbial populations and subpopulations did not correlate with the inhibitory effects of the teas. The diversity of microbial populations or the presence of particular microbial species is more likely to be a determining factor of compost tea efficacy.
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References
Al-Dahmani, J. H., Abbasi, P. A., Miller, S. A., & Hoitink, H. A. J. (2003). Suppression of bacterial spot of tomato with foliar sprays of compost extracts under greenhouse and field conditions. Plant Disease, 87, 913–919. https://doi.org/10.1094/pdis.2003.87.8.913
Barghouth, Z., Khazzam, E., Ramlawi, S., Wong, A., Smith, M. L., & Avis, T. J. (2023). Microbial compost tea properties affect suppression of strawberry grey mould (Botrytis cinerea Pers.). Biocontrol Science and Technology, 33, 1–18. https://doi.org/10.1080/09583157.2022.2141688
Bautista-Rosales, P. U., Calderon-Santoyo, M., Servín-Villegas, R., Ochoa-Álvarez, N. A., & Ragazzo-Sánchez, J. A. (2013). Action mechanisms of the yeast Meyerozyma caribbica for the control of the phytopathogen Colletotrichum gloeosporioides in mangoes. Biological Control, 65, 293–301. https://doi.org/10.1016/j.biocontrol.2013.03.010
Carvajal, M., Olivares, M., Lobaina, E., Vergara, A., Velasquez, A., Jeldres, P., Meza, D., Dorta, F., Jorquera, F., & Seeger, M. (2023). Addition of Trichoderma consortia to Chilean endemic flora compost teas strongly enhances in vitro and in vivo biocontrol of phytopathogenic fungi. Journal of Applied Microbiology, 134, 1–13. https://doi.org/10.1093/jambio/lxad011
Chen, X., Wang, Y., Gao, Y., Gao, T., & Zhang, D. (2019). Inhibitory abilities of Bacillus isolates and their culture filtrates against the gray mold caused by Botrytis cinerea on postharvest fruit. Plant Pathology Journal, 35, 425–436. https://doi.org/10.5423/ppj.oa.03.2019.0064
Collinge, D. B., Jensen, D. F., Rabiey, M., Sarrocco, S., Shaw, M. W., & Shaw, R. H. (2022). Biological control of plant diseases - what has been achieved and what is the direction? Plant Pathology, 71, 1024–1047. https://doi.org/10.1111/ppa.13555
Dean, R., Van Kan, J. A. L., Pretorius, Z. A., Hammond-Kosack, K. E., Di Pietro, A., Spanu, P. D., Rudd, J. J., Dickman, M., Kahmann, R., Ellis, J., & Foster, G. D. (2012). The Top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13, 414–430. https://doi.org/10.1111/j.1364-3703.2011.00783.x
Dianez, F., Marin, F., Santos, M., Gea, F. J., Navarro, M. J., Pineiro, M., & Gonzalez, J. M. (2018). Genetic analysis and in vitro enzymatic determination of bacterial community in compost teas from different sources. Compost Science & Utilization, 26, 256–270. https://doi.org/10.1080/1065657x.2018.1496045
Dimkić, I., Berić, T., Stević, T., Pavlović, S., Šavikin, K., Fira, D., & Stanković, S. (2015). Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds. Biological Control, 87, 6–13. https://doi.org/10.1016/j.biocontrol.2015.04.011
Dionne, A., Tweddell, R. J., Antoun, H., & Avis, T. J. (2012). Effect of non-aerated compost teas on damping-off pathogens of tomato. Canadian Journal of Plant Pathology, 34, 51–57. https://doi.org/10.1080/07060661.2012.660195
Elad, Y., & Shtienberg, D. (1994). Effect of compost water extracts on gray mold (Botrytis cinerea). Crop Protection, 13, 109–114. https://doi.org/10.1016/0261-2194(94)90160-0
FAO, IFAD, UNICEF, WFP, & WHO. (2023). The State of Food Security and Nutrition in the World 2023. Urbanization, agrifood systems transformation and healthy diets across the rural–urban continuum. Rome, FAO. https://doi.org/10.4060/cc3017en
Koné, S. B., Dionne, A., Tweddell, R. J., Antoun, H., & Avis, T. J. (2010). Suppressive effect of non-aerated compost teas on foliar fungal pathogens of tomato. Biological Control, 52, 167–173. https://doi.org/10.1016/j.biocontrol.2009.10.018
Kretschmer, M., Leroch, M., Mosbach, A., Walker, A.-S., Fillinger, S., Mernke, D., Schoonbeek, H. J., Pradier, J. M., Leroux, P., De Waard, M. A., & Hahn, M. (2009). Fungicide-driven evolution and molecular basis of multidrug resistance in field populations of the grey mould fungus Botrytis cinerea. PLOS Pathogens, 5, 1000696. https://doi.org/10.1371/journal.ppat.1000696
Kurniawan, O., Wilson, K., Mohamed, R., & Avis, T. J. (2018). Bacillus and Pseudomonas spp. provide antifungal activity against gray mold and Alternaria rot on blueberry fruit. Biological Control, 126, 136–141. https://doi.org/10.1016/j.biocontrol.2018.08.001
Li, X., Wang, X., Shi, X., Wang, Q., Li, X., & Zhang, S. (2020). Compost tea-mediated induction of resistance in biocontrol of strawberry Verticillium wilt. Journal of Plant Diseases and Protection, 127, 257–268. https://doi.org/10.1007/s41348-019-00290-0
Litterick, A. M., Harrier, L., Wallace, P., Watson, C. A., & Wood, M. (2004). The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production - a review. Critical Reviews in Plant Sciences, 23, 453–479. https://doi.org/10.1080/07352680490886815
Mari, M., Martini, C., Guidarelli, M., & Neri, F. (2012). Postharvest biocontrol of Monilinia laxa, Monilinia fructicola and Monilinia fructigena on stone fruit by two Aureobasidium pullulans strains. Biological Control, 60, 132–140. https://doi.org/10.1016/j.biocontrol.2011.10.013
Marin, F., Santos, M., Dianez, F., Carretero, F., Gea, F. J., Yau, J. A., & Navarro, M. J. (2013). Characters of compost teas from different sources and their suppressive effect on fungal phytopathogens. World Journal of Microbiology & Biotechnology, 29, 1371–1382. https://doi.org/10.1007/s11274-013-1300-x
Mengesha, W. K., Powell, S. M., Evans, K. J., & Barry, K. M. (2017). Diverse microbial communities in non-aerated compost teas suppress bacterial wilt. World Journal of Microbiology & Biotechnology, 33, 49. https://doi.org/10.1007/s11274-017-2212-y
On, A., Wong, F., Ko, Q., Tweddell, R. J., Antoun, H., & Avis, T. J. (2015). Antifungal effects of compost tea microorganisms on tomato pathogens. Biological Control, 80, 63–69. https://doi.org/10.1016/j.biocontrol.2014.09.017
Onyeaka, H. N., & Nwabor, O. F. (2022). Natural bioactive compounds in food production and preservation. In H. N. Onyeaka & O. F. Nwabor (Eds.), Food Preservation and Safety of Natural Products (pp. 57–73). Academic Press. https://doi.org/10.1016/B978-0-323-85700-0.00006-X
Orozco-Mosqueda, M. D. C., Kumar, A., Fadiji, A. E., Babalola, O. O., Puopolo, G., & Santoyo, G. (2023). Agroecological management of the grey mould fungus Botrytis cinerea by plant growth-promoting bacteria. Plants, 12, 637. https://doi.org/10.3390/plants12030637
Palmer, A. K., Evans, K. J., & Metcalf, D. A. (2010). Characters of aerated compost tea from immature compost that limit colonization of bean leaflets by Botrytis cinerea. Journal of Applied Microbiology, 109, 1619–1631. https://doi.org/10.1111/j.1365-2672.2010.04794.x
Pane, C., Celano, G., Villecco, D., & Zaccardelli, M. (2012). Control of Botrytis cinerea, Alternaria alternata and Pyrenochaeta lycopersici on tomato with whey compost-tea applications. Crop Protection, 38, 80–86. https://doi.org/10.1016/j.cropro.2012.03.012
Pane, C., Celano, G., & Zaccardeli, M. (2014). Metabolic patterns of bacterial communities in aerobic compost teas associated with potential biocontrol of soilborne plant diseases. Phytopathologia Mediterranea, 53, 277–286. https://doi.org/10.14601/Phytopathol_Mediterr-13363
Parafati, L., Vitale, A., Restuccia, C., & Cirvilleri, G. (2015). Biocontrol ability and action mechanism of food-isolated yeast strains against Botrytis cinerea causing post-harvest bunch rot of table grape. Food Microbiology, 47, 85–92. https://doi.org/10.1016/j.fm.2014.11.013
Platania, C., Restuccia, C., Muccilli, S., & Cirvilleri, G. (2012). Efficacy of killer yeasts in the biological control of Penicillium digitatum on Tarocco orange fruits (Citrus sinensis). Food Microbiology, 30, 219–225. https://doi.org/10.1016/j.fm.2011.12.010
Ramlawi, S., Chiu, J. O., Cloutier, A., & Avis, T. J. (2021). Suppression of Fusarium dry rot of potato using beneficial bacterial treatments. Journal of Plant Pathology, 103, 269–281. https://doi.org/10.1007/s42161-020-00731-y
Samet, M., Karray, F., Mhiri, N., Kamoun, L., Sayadi, S., & Gargouri-Bouzid, R. (2019). Effect of phosphogypsum addition in the composting process on the physico-chemical proprieties and the microbial diversity of the resulting compost tea. Environmental Science and Pollution Research, 26, 21404–21415. https://doi.org/10.1007/s11356-019-05327-3
Sautua, F. J., Baron, C., Perez-Hernandez, O., & Carmona, M. A. (2019). First report of resistance to carbendazim and procymidone in Botrytis cinerea from strawberry, blueberry and tomato in Argentina. Crop Protection, 125, 104879. https://doi.org/10.1016/j.cropro.2019.104879
Savary, S., Willocquet, L., Pethybridge, S. J., Esker, P., McRoberts, N., & Nelson, A. (2019). The global burden of pathogens and pests on major food crops. Nature Ecology & Evolution, 3, 430–439. https://doi.org/10.1038/s41559-018-0793-y
Scheuerell, S. J., & Mahaffee, W. F. (2006). Variability associated with suppression of gray mold (Botrytis cinerea) on geranium by foliar applications of nonaerated and aerated compost teas. Plant Disease, 90, 1201–1208. https://doi.org/10.1094/pd-90-1201
Shao, W., Zhao, Y., & Ma, Z. (2021). Advances in understanding fungicide resistance in Botrytis cinerea in China. Phytopathology, 111, 455–463. https://doi.org/10.1094/phyto-07-20-0313-ia
Sharma, E., & Kapoor, R. (2017). Insights into the molecular interplay of virulence factors in Botrytis cinerea. Australasian Plant Pathology, 46, 551–561. https://doi.org/10.1007/s13313-017-0519-7
Siddiqui, Y., Naidu, Y., & Ali, A. (2015). Bio-intensive management of fungal diseases of fruits and vegetables utilizing compost and compost teas. In M. K. Meghvansi & A. Varma (Eds.), Organic Amendments and Soil Suppressiveness in Plant Disease Management, (vol. 46, pp. 307–329). Springer. https://doi.org/10.1007/978-3-319-23075-7_14
Sofianos, G., Samaras, A., & Karaoglanidis, G. (2023). Multiple and multidrug resistance in Botrytis cinerea: Molecular mechanisms of MLR/MDR strains in Greece and effects of co-existence of different resistance mechanisms on fungicide sensitivity. Frontiers in Plant Science, 14, 1273193. https://doi.org/10.3389/fpls.2023.1273193
St Martin, C. C. G., Dorinvil, W., Brathwaite, R. A. I., & Ramsubhag, A. (2012). Effects and relationships of compost type, aeration and brewing time on compost tea properties, efficacy against Pythium ultimum, phytotoxicity and potential as a nutrient amendment for seedling production. Biological Agriculture & Horticulture, 28, 185–205. https://doi.org/10.1080/01448765.2012.727667
Stewart-Wade, S. M. (2020). Efficacy of organic amendments used in containerized plant production: Part 1-compost-based amendments. Scientia Horticulturae, 266, 108856. https://doi.org/10.1016/j.scienta.2019.108856
Villecco, D., Pane, C., Ronga, D., & Zaccardelli, M. (2020). Enhancing sustainability of tomato, pepper and melon nursery production systems by using compost tea spray applications. Agronomy, 10(1336), 1336. https://doi.org/10.3390/agronomy10091336
Zhao, J., Bi, Q., Wu, J., Lu, F., Han, X., & Wang, W. (2019). Occurrence and management of fungicide resistance in Botrytis cinerea on tomato from greenhouses in Hebei, China. Journal of Phytopathology, 167, 413–421. https://doi.org/10.1111/jph.12812
Acknowledgements
We thank Edward Bruggink and Michael Shaikhet for technical assistance.
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This research was supported by grant RGPIN-2020–06406 from the Natural Sciences and Engineering Council of Canada (NSERC) to TJA.
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CB and IP prepared the original draft, performed material preparation, data collection and analysis; HS performed material preparation, data collection and analysis; CB and MLS conceived some experiments; TJA conceptualized the project and methodology, performed data analysis, supervised and administered the project, acquired project funding. All authors reviewed and approved the final manuscript.
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Bakker, C., Popescu, I., Schott, H. et al. Compost teas provide reduction of grey mould (Botrytis cinerea Pers.) on tomato plants. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02860-x
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DOI: https://doi.org/10.1007/s10658-024-02860-x