Abstract
Isolation on culture media followed with macroscopic, microscopic, molecular and phylogenetic analyses and pathogenicity tests allowed us to identify Pythium capillosum to be capable of destroying dagger nematode through endozoic parasitism from ingested oomycete zoospores. Although endoparasitism of free-living nematodes is found to be common among oomycetes in nature, this is the first finding of such occurrence in Bosnia and Herzegovina soils. The obtained results could open the door to future studies for controlling nematodes in an environmentally safe and sustainable way.
This is a preview of subscription content, log in via an institution to check access.
References
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2
Bileva, T., Choleva, B., Hockland, S., Ciancio, A. (2009). Management Of Virus-Transmitting Nematodes with special emphasis on South-East Europe. Book chapter Ciancio A., Mukerji K. G. (eds.), Integrated management of fruit crops and forest nematodes, 215–242. Springer Science+Business Media B.V. 2009. https://doi.org/10.1007/978-1-4020-9858-1_9.
Book chapter Arora D. K. (2004). Fungal Biotechnology in Agricultural, Food, and Environmental Applications. In Jansson H.-B. and Lopez-Llorca L. V. (2004) Control of Nematodes by Fungi. In book: Fungal Biotechnology in Agricultural, Food, and Environmental Applications Publisher: Marcel Dekker. https://doi.org/10.1201/9780203913369.ch18.
Brown, D. J. F., & Boag, B. (1988). An examination of methods used to extract virus-vector nematodes (Nematoda: Longidoridae and Trichodoridae) from soil samples. Nematologia Mediterranea, 16, 93–99.
Czeczuga, B., & Muszyńska, E. (1999). Aquatic fungi growing on percid fish eggs (Percidae) in Poland. Polish Journal of Environmental Studies, 8(1), 31–34.
Devi, G. (2018). Utilization of nematode destroying fungi for management of plant parasitic nematodes - a review. Biosciences Biotechnology Research Asia, 15(2), 377–396.
Hendrix, F.F., & Papa K.E. (1974). Symposium on the genus Pythium. Taxonomy and genetics of Pythium Proceedings of the American Phytopathological Society, pp. 200–207
Hyde, K. D., Nilsson, R. H., Alias, S. A., Ariyawansa, H. A., Blair, J. E., Cai, L., de Cock, A. W. A. M., Dissanayake, A. J., Glockling, S. L., Goonasekara, I. D., Gorczak, M., Hahn, M., Jayawardena, R. S., van Kan, J. A. L., Laurence, M. H., Lévesque, C. A., Li, X., Liu, J. K., Maharachchikumbura, S. S. N., … Zhou, N. (2014). One stop shop: Backbones trees for important phytopathogenic genera: I. Fungal Diversity, 67, 21–125. https://doi.org/10.1007/s13225-014-0298-1
Lévesque, C. A., & de Cock, A. W. (2004). Molecular phylogeny and taxonomy of the genus Pythium. Mycological Research, 108(12), 1363–83. https://doi.org/10.1017/s0953756204001431
Orlando, V., Chitambar, J. J., Dong, K., Chizhov, V. N., Mollov, D., Bert, W., & Subbotin, S. A. (2016). Molecular and morphological characterisation of Xiphinema americanum-group species (Nematoda: Dorylaimida) from California, USA, and Other Regions, and Co-Evolution of Bacteria from the Genus Candidatus Xiphinematobacter with Nematodes. Nematology, 18(9), 1015–1043. https://doi.org/10.1163/15685411-00003012
Paul, B. (1989). A new species of Pythium with filamentous sporangia from Algeria. Transactions of the British Mycological Society, 89(2), 195–198. https://doi.org/10.1016/S0007-1536(87)80152-3
Plaats-Niterink, A. J. van der (1968). The occurrence of Pythium in the Netherlands I.Heterothallic species. Acta Botanica Neerlandica, 17, 320–329.
Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(7), 3022–3027. https://doi.org/10.1093/molbev/msab120
Timper, P. (2011). Utilization of biological control for managing plant-parasitic nematodes. In: Biological control of plant-parasitic nematodes: building coherence between microbial ecology and molecular mechanisms. Progress in biological control (Davies KG and Spiegel Y, eds.). Dordrecht, the Netherlands: Springer Science and Business Media, pp 34–45.
Tranier, M.S., Pognant-Gros, J., Quiroz, R. DeC, Gonzalez, C.A.N, Mateille, T., Roussos, S. (2014). Commercial biological control agents targeted against plant-parasitic root-knot nematodes. Brazilian Archives of Biology and Technology, 57(6): Curitiba Nov./Dec.
Tzean, S. S., & Estey, R. H. (1981). Species of Phytophthora and Pythium as Nematode-destroying Fungi. Journal of Nematology, 13(2), 160–163.
Acknowledgements
This work is partially done through collaboration on Erasmus+ Mobility project for higher education students and staff No. 2020-1-SK01-KA107-077779 and EU4FITO-BiH twining project.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Hereby we confirm and declare that in the work done and present in this paper there is no any potential conflict of interest, also in the research any human and/or animals’ participant wasn’t used and there is no any disagreement with informed consent.
Additional information
R. Milašin, M. Voruna and S. Matić equally participated in the work.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Milašin, R., Voruna, M., Matić, S. et al. Characterisation of Pythium capillosum – A new pathogen of Xiphinema pachtaicum (Nematoda: Longidoridae). Eur J Plant Pathol 168, 497–500 (2024). https://doi.org/10.1007/s10658-023-02778-w
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-023-02778-w