Abstract
Early blight caused by Alternaria solani is a serious threat to crop production. In this study, the synergistic antagonism mechanism of a biocontrol consortium (including Bacillus subtilis J3 and Pseudomonas fluorescens J8) isolated from the tomato rhizosphere against A.solani was investigated. The consortium (109 CFU/mL, J3:J8(v/v) = 2:3) efficiently inhibited A.solani growth with an inhibition rate of 94.10%, significantly higher than the single culture of J3 or J8 (P < 0.05). The co-cultivation of J3 and J8 significantly enhanced (6.51-146.81%) the expression of the functional genes related to the production of antimicrobial substances, including srfA, fenA, pvds, phlA, and hcnA. Siderophore and 2,4-diacetylphloroglucinol (2,4-DAPG) were 55.45-70.18% higher in the consortium than in single strains, significantly contributing to pathogen biocontrol. The interchange of metabolic substances between the bacteria probably improved their performance. This paper presents useful biocontrol bacteria material and provides important information on the synergistic mechanism of the bacteria, which could help to design and implement such biocontrol agents.
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The data used to support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgments
We are thankful to Dr. Yong Wang, School of Plant Protection, Northwest A&F University for providing the Alternaria solani strain for this study. We are grateful to Dr. Weizhen Fang of the Analysis & Testing center, Southwest Jiaotong University for the technical support. We are also grateful to Mr. Yu Wang and Mr. Hang Qiu for their help in the initial writing.
Contributions
JYX conceived and designed the study. LJP, NH, MHT, HQ and LB revised the manuscript. QZP and WC provide fund support and supervision. LB, QZP and WC share the corresponding authorship. All authors read and approved the manuscript.
Funding
This work was financially supported by the National Natural Science Foundation of China (Grant No. 51978576), the Key Research and Development Plan of Sichuan Province (23SYSX0128, 23ZHSF0040), the Science and Technology Project of Chengdu (2022-YF05-00909-SN, 2022-YF05-00269-SN), Science and Technology Project of Sichuan Tobacco Company of China National Tobacco Corporation (SCYC202109).
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• Pseudomonas-Bacillus co-cultivation synergistically antagonized Alternaria solani;
• Overexpression of genes related to antibiotic production in the consortium;
• Antibiotic production increased in the consortium;
• Growth promoting and disease control were obtained with the consortium.
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Jia, Y., Li, J., Niu, H. et al. Synergistic antagonism mechanism of Bacillus-Pseudomonas consortium against Alternaria solani. Eur J Plant Pathol 167, 715–726 (2023). https://doi.org/10.1007/s10658-023-02747-3
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DOI: https://doi.org/10.1007/s10658-023-02747-3