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Effect of antagonistic bacteria associated with canola on disease suppression

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Abstract

Antagon istic bacteria can be used as a safe and nature-friendly method of disease control. Bacteria associated with canola plants from western Iranian fields were isolated and their inhibitory effect against the blackleg pathogen of canola investigated in vitro. 8 isolates were selected based on antagonistic test results (dual cultures) and in vitro study of biocontrol mechanism. Next, their inhibitory ability against blackleg disease in the greenhouse was investigated. Selected isolates showed high inhibitory ability against Leptosphaeria maculans in vitro, suggesting the susceptibility of the blackleg pathogen to antifungal compounds produced by the selected strains. The selected isolates were able to produce antifungal antibiotics. Antifungal volatile compounds were observed in six selected isolates. All eight selected isolates generated hydrolytic enzymes such as cellulase, lipase, and amylase. Moreover, six isolates were able to produce protease. Isolate 642 (confirmed as Pseudomonas sp.) significantly inhibited blackleg disease under greenhouse conditions, suggesting its suitability for further investigation in the field.

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Acknowledgements

The authors would like to thank the Lorestan University for financial support and from the Bu-Ali Sina University for supporting experimental facilities.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

    Azadeh Jamalzadeh, Mostafa Darvishnia & Eydi Bazgir

  2. Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

    Gholam Khodakaramian & Doostmorad Zafari

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  1. Azadeh Jamalzadeh
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Correspondence to Mostafa Darvishnia.

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Jamalzadeh, A., Darvishnia, M., Khodakaramian, G. et al. Effect of antagonistic bacteria associated with canola on disease suppression. Eur J Plant Pathol 165, 649–663 (2023). https://doi.org/10.1007/s10658-022-02633-4

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