Abstract
Broad bean wilt virus 1 (BBWV-1) from the genus Fabavirus (family Secoviridae) infects many important crops worldwide. We have studied the subcellular alterations in leaf tissues of Nicotiana benthamiana and broad bean (Vicia faba) plants infected by BBWV-1 using transmission electron microscopy (TEM). In addition, we have studied the effect at the subcellular level of the BBWV-1 VP37 protein, which is a pathogenicity and host specificity determinant. For this, we agroinfiltrated N. benthamiana, and broad bean plants with two BBWV-1 infective clones, the wild type pBBWV1-wt and pBBWV1-G492C defective for VP37. Mesophyll cells infected with BBWV1-wt showed many subcellular alterations such as starch accumulation and plastoglobuli in chloroplasts, cytoplasmic vesiculation, plasmalemmasomes, vacuolar inclusion bodies, and presence of virus-like particles (VLPs) scattered in the cytoplasm in both N. benthamiana and broad bean. As differential effects, aggregated VLPs in vacuolar inclusion bodies and mitochondrial proliferation were observed in N. benthamiana but not in broad bean. TEM observation of pBBWV1-G492C infected tissues suggested that VP37 could be involved in the starch accumulation in both N. benthamiana and broad bean, and with the presence of aggregated VLPs in vacuolar inclusion bodies, plasmalemmasomes, and mitochondrial proliferation in N. benthamiana. Thus, subcellular alterations induced by BBWV-1 depended on the host species and the effect of VP37 expression was higher in N. benthamiana than in broad bean.
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Acknowledgments
This research was supported by the project PROY-IVIA-2013/14 n°5426 founded by the Valencian Institute of Agricultural Research. TEM analysis was conducted in the Electron Microscopy Service of Polytechnic University of Valencia.
Funding
This research work was supported by funds of the Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain (PROY-IVIA-2013/14 n°5426). C. Carpino was a recipient of predoctoral fellowships from the Italian government.
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Vicente Medina performed the TEM analysis. Caterina Carpino and Laura Elvira-Gonzalez prepared the plant material for TEM analysis. Vicente Medina and Luis Rubio advised on methods and analysis and critically revised the paper. Luis Galipienso is responsible for experimental design and paper writing and edition.
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The authors certify that we do not have any actual or potential conflict of interest. The study presented here is original and has not been published previously. All authors have reviewed the manuscript and approved its final version before submission.
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Medina, V., Carpino, C., Elvira-González, L. et al. Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants. Eur J Plant Pathol 164, 451–459 (2022). https://doi.org/10.1007/s10658-022-02564-0
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DOI: https://doi.org/10.1007/s10658-022-02564-0